Creating Versatile Metallo-Enzyme Environments for Selective C-H Activation Chemistry: Lignocellulose Deconstruction and Beyond

Project Information

enzC-Hem

Grant agreement ID: 757991

DOI 10.3030/757991

Project closed

EC signature date 13 November 2017

Start date 1 January 2018

End date 31 December 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 492 424,00

EU contribution € 1 492 424,00

Coordinated by

THE UNIVERSITY OF MANCHESTER
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (23)

Engineering an efficient and enantioselective enzyme for the Morita–Baylis–Hillman reaction

Author(s): Rebecca Crawshaw, Amy E. Crossley, Linus Johannissen, Ashleigh J. Burke, Sam Hay, Colin Levy, David Baker, Sarah L. Lovelock, Anthony P. Green
Published in: Nature Chemistry, Issue 14, 2023, Page(s) 313-320, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-021-00833-9

Insights into the H2O2-driven catalytic mechanism of fungal lytic polysaccharide monooxygenases

Author(s): Tobias M. Hedison; Erik Breslmayr; Erik Breslmayr; Muralidharan Shanmugam; Kwankao Karnpakdee; Derren J. Heyes; Anthony P. Green; Roland Ludwig; Nigel S. Scrutton; Daniel Kracher; Daniel Kracher
Published in: Hedison , T M , Breslmayr , E , Shanmugam , M , Karnpakdee , K , Heyes , D J , Green , A P , Ludwig , R , Scrutton , N S & Kracher , D 2020 , ' Insights into the H2O2 ‐driven catalytic mechanism of fungal lytic polysaccharide monooxygenases ' , The FEBS Journal . https://doi.org/10.1111/febs.15704, Issue 17424658, 2021, ISSN 1742-4658
Publisher: Wiley
DOI: 10.1111/febs.15704

The road to fully programmable protein catalysis

Author(s): Sarah L. Lovelock, Rebecca Crawshaw, Sophie Basler, Colin Levy, David Baker, Donald Hilvert, Anthony P. Green
Published in: Nature, Issue 606, 2022, Page(s) 49-58, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-022-04456-z

An efficient pyrrolysyl-tRNA synthetase for economical production of MeHis-containing enzymes

Author(s): Amy E. Hutton, Jake Foster, James E. J. Sanders, Christopher J. Taylor, Stefan A. Hoffmann, Yizhi Cai, Sarah L. Lovelock, Anthony P. Green
Published in: Faraday Discussions, Issue 252, 2024, Page(s) 295-305, ISSN 1359-6640
Publisher: Royal Society of Chemistry
DOI: 10.1039/d4fd00019f

Enzymes with noncanonical amino acids

Author(s): Jingming Zhao, Ashleigh J. Burke, Anthony P. Green
Published in: Current Opinion in Chemical Biology, Issue 55, 2020, Page(s) 136-144, ISSN 1367-5931
Publisher: Elsevier BV
DOI: 10.1016/j.cbpa.2020.01.006

Strategies for designing biocatalysts with new functions

Author(s): Elizabeth L. Bell, Amy E. Hutton, Ashleigh J. Burke, Adam O’Connell, Amber Barry, Elaine O’Reilly, Anthony P. Green
Published in: Chemical Society Reviews, Issue 53, 2024, Page(s) 2851-2862, ISSN 0306-0012
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3cs00972f

Noncanonical Amino Acids in Biocatalysis

Author(s): Zachary Birch-Price, Florence J. Hardy, Thomas M. Lister, Anna R. Kohn, Anthony P. Green
Published in: Chemical Reviews, Issue 124, 2024, Page(s) 8740-8786, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.4c00120

A Noncanonical Tryptophan Analogue Reveals an Active Site Hydrogen Bond Controlling Ferryl Reactivity in a Heme Peroxidase.

Author(s): Mary Ortmayer; Florence J. Hardy; Matthew G. Quesne; Matthew G. Quesne; Karl Fisher; Colin Levy; Derren J. Heyes; C. Richard A. Catlow; C. Richard A. Catlow; C. Richard A. Catlow; Sam P. de Visser; Stephen E. J. Rigby; Sam Hay; Anthony P. Green
Published in: 2691-3704, Issue 26913704, 2021, ISSN 2691-3704
Publisher: American Chemical Society
DOI: 10.1021/jacsau.1c00145

Mapping the Initial Stages of a Protective Pathway that Enhances Catalytic Turnover by a Lytic Polysaccharide Monooxygenase

Author(s): Jingming Zhao, Ying Zhuo, Daniel E. Diaz, Muralidharan Shanmugam, Abbey J. Telfer, Peter J. Lindley, Daniel Kracher, Takahiro Hayashi, Lisa S. Seibt, Florence J. Hardy, Oliver Manners, Tobias M. Hedison, Katherine A. Hollywood, Reynard Spiess, Kathleen M. Cain, Sofia Diaz-Moreno, Nigel S. Scrutton, Morten Tovborg, Paul H. Walton, Derren J. Heyes, Anthony P. Green
Published in: Journal of the American Chemical Society, Issue 145, 2023, Page(s) 20672-20682, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c06607

Rewiring the “Push-Pull” Catalytic Machinery of a Heme Enzyme Using an Expanded Genetic Code

Author(s): Mary Ortmayer, Karl Fisher, Jaswir Basran, Emmanuel M. Wolde-Michael, Derren J. Heyes, Colin Levy, Sarah L. Lovelock, J. L. Ross Anderson, Emma L. Raven, Sam Hay, Stephen E. J. Rigby, Anthony P. Green
Published in: ACS Catalysis, Issue 10/4, 2020, Page(s) 2735-2746, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.9b05129

Engineering enzyme activity using an expanded amino acid alphabet

Author(s): Zachary Birch-Price, Christopher J Taylor, Mary Ortmayer, Anthony P Green
Published in: Protein Engineering, Design and Selection, Issue 36, 2023, ISSN 1741-0126
Publisher: Oxford University Press
DOI: 10.1093/protein/gzac013

Engineered Metalloenzymes with Non‐Canonical Coordination Environments

Author(s): Takahiro Hayashi, Donald Hilvert, Anthony P. Green
Published in: Chemistry – A European Journal, Issue 24/46, 2018, Page(s) 11821-11830, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201800975

Design and evolution of an enzyme with a non-canonical organocatalytic mechanism

Author(s): Ashleigh J. Burke, Sarah L. Lovelock, Amina Frese, Rebecca Crawshaw, Mary Ortmayer, Mark Dunstan, Colin Levy, Anthony P. Green
Published in: Nature, Issue 570/7760, 2019, Page(s) 219-223, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-019-1262-8

A Noncanonical Proximal Heme Ligand Affords an Efficient Peroxidase in a Globin Fold

Author(s): Moritz Pott, Takahiro Hayashi, Takahiro Mori, Peer R. E. Mittl, Anthony P. Green, Donald Hilvert
Published in: Journal of the American Chemical Society, Issue 140/4, 2018, Page(s) 1535-1543, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.7b12621

Chemo-Enzymatic Synthesis of Pyrazines and Pyrroles

Author(s): Jin Xu, Anthony P. Green, Nicholas J. Turner
Published in: Angewandte Chemie International Edition, Issue 57/51, 2018, Page(s) 16760-16763, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201810555

A designed photoenzyme for enantioselective [2+2] cycloadditions

Author(s): Jonathan S. Trimble, Rebecca Crawshaw, Florence J. Hardy, Colin W. Levy, Murray J. B. Brown, Douglas E. Fuerst, Derren J. Heyes, Richard Obexer, Anthony P. Green
Published in: Nature, Issue 611, 2022, Page(s) 709-714, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-022-05335-3

Design of Heme Enzymes with a Tunable Substrate Binding Pocket Adjacent to an Open Metal Coordination Site

Author(s): Indrek Kalvet, Mary Ortmayer, Jingming Zhao, Rebecca Crawshaw, Nathan M. Ennist, Colin Levy, Anindya Roy, Anthony P. Green, David Baker
Published in: Journal of the American Chemical Society, Issue 145, 2023, Page(s) 14307-14315, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c02742

Engineering mutually orthogonal <scp>PylRS</scp>/<scp>tRNA</scp> pairs for dual encoding of functional histidine analogues

Author(s): Christopher J. Taylor, Florence J. Hardy, Ashleigh J. Burke, Riley M. Bednar, Ryan A. Mehl, Anthony P. Green, Sarah L. Lovelock
Published in: Protein Science, Issue 32, 2023, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4640

Biocatalysis

Author(s): Elizabeth L. Bell, William Finnigan, Scott P. France, Anthony P. Green, Martin A. Hayes, Lorna J. Hepworth, Sarah L. Lovelock, Haruka Niikura, Sílvia Osuna, Elvira Romero, Katherine S. Ryan, Nicholas J. Turner, Sabine L. Flitsch
Published in: Nature Reviews Methods Primers, Issue 1, 2024, ISSN 2662-8449
Publisher: Nature Publishing Group
DOI: 10.1038/s43586-021-00044-z

A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme

Author(s): Amy E. Hutton, Jake Foster, Rebecca Crawshaw, Florence J. Hardy, Linus O. Johannissen, Thomas M. Lister, Emilie F. Gérard, Zachary Birch-Price, Richard Obexer, Sam Hay, Anthony P. Green
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-46123-z

Probing Ferryl Reactivity in a Nonheme Iron Oxygenase Using an Expanded Genetic Code

Author(s): Florence J. Hardy, Matthew G. Quesne, Emilie F. Gérard, Jingming Zhao, Mary Ortmayer, Christopher J. Taylor, Hafiz S. Ali, Jeffrey W. Slater, Colin W. Levy, Derren J. Heyes, J. Martin Bollinger, Sam P. de Visser, Anthony P. Green
Published in: ACS Catalysis, Issue 14, 2024, Page(s) 11584-11590, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.4c02365

An Engineered Cytidine Deaminase for Biocatalytic Production of a Key Intermediate of the Covid-19 Antiviral Molnupiravir

Author(s): Ashleigh J. Burke; William R. Birmingham; Ying Zhuo; Thomas W. Thorpe; Bruna Zucoloto da Costa; Rebecca Crawshaw; Ian Rowles; James D. Finnigan; Carl Young; Gregory M. Holgate; Mark P. Muldowney; Simon J. Charnock; Sarah L. Lovelock; Nicholas J. Turner; Anthony P. Green
Published in: Crossref, Issue 26913704, 2022, ISSN 2691-3704
Publisher: American Chemical Society
DOI: 10.1021/jacs.1c11048

Directed evolution of an efficient and thermostable PET depolymerase

Author(s): Elizabeth L. Bell, Ross Smithson, Siobhan Kilbride, Jake Foster, Florence J. Hardy, Saranarayanan Ramachandran, Aleksander A. Tedstone, Sarah J. Haigh, Arthur A. Garforth, Philip J. R. Day, Colin Levy, Michael P. Shaver, Anthony P. Green
Published in: Nature Catalysis, Issue 5, 2022, Page(s) 673-681, ISSN 2520-1158
Publisher: Nature Publishing
DOI: 10.1038/s41929-022-00821-3

Non-peer reviewed articles (1)

A Designed Photoenzyme Promotes Enantioselective [2+2]-Cycloadditions via Triplet Energy Transfer

Author(s): Anthony Green; Jonathan Trimble; Rebecca Crawshaw; Florence Hardy; Colin Levy; Murray Brown; Douglas Fuerst; Derren Heyes; Richard Obexer
Published in: Crossref, Issue 25732293, 2022, ISSN 2573-2293
Publisher: Cambridge University Press
DOI: 10.21203/rs.3.rs-1650767/v1