We carried out a feasibility study examining both technical feasibility and business viability of the project. We made preparation for clinical study. We made a clinical trial design, determined the gold standards and outlined ethical considerations for the clinical study. We selected some potential clinics. In addition, we validated our business assumptions by structured interviews with men’s fertility specialists (andrologists) and medical laboratory service providers. Furthermore, we performed a risk assessment and suggested mitigations for the risks. We have also completed our business plan based on business model canvas, including our company strategy, commercial model, financial scale-up plan, organisational growth plan. We also made an analysis on IP protection, regulatory issues and freedom to operate. Finally, we made a detailed plan for a further grant in the EIC-Accelerator Pilot including both grant and equity component.