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Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes

Deliverables

Data management plan

Generation of a Data Management Plan to follow the Horizon 2020 Open Research Data Pilot guidelines

Updated data management plan

Update of the Data Management Plan

Intermediate dissemination and exploitation report

This document will report on the plan envisaged by the consortium for project results dissemination and exploitation

Report on exosome-specific Ab selection

This document will report on the selection of the panel of antibodies for on-chip exosome immuno- phenotyping

Final dissemination and exploitation report

This document will report on the dissemination activities held during the project and on the possibility of exploitation of results and deliverables for translation of inventions obtained during the project.

Report on clinical demonstration

This document will report on the results obtained using the newly developed instrumentation with selected clinical samples

Report on criteria for system demonstration

This report will provide the specifications on the requested performance of the systems and the criteria that will be used for system demonstration at the end of the project.

Publications

Computational nanosensing from defocus in single particle interferometric reflectance microscopy

Author(s): Yurdakul, C., M.S., Ünlü
Published in: OpticsLetters, 2020
Publisher: OpticsLetters
DOI: 10.1364/ol.409458

Separation of Extracellular Vesicles by DNA-Directed Immunocapturing Followed by Enzymatic Release

Author(s): Brambilla, D., Sola, L., Chiodi, E., Zarovni, N., Fortunato, D., Criscuoli, M., Dolo, V., Giusti, I., Murdica, V., Vago, R., Chiari, M.
Published in: ChemRxiv, 2020
Publisher: ChemRxiv
DOI: 10.26434/chemrxiv.12234683.v1

Multiplexed affinity measurements of extracellular vesicles binding kinetics

Author(s): Chiodi, E.; Daaboul, G.; Marn, A.M.; Ünlü, M.S.
Published in: ChemRxiv, 2021
Publisher: ChemRxiv
DOI: 10.26434/chemrxiv.13547459.v1

A Bi-Functional Polymeric Coating for the Co-Imobilization of Proteins and Peptides on Microarray Substrates

Author(s): Laura Sola, Dario Brambilla, Alessandro Mussida, Roberto Consonni, Francesco Damin, Marina Cretich, Alessandro Gori, Marcella Chiari
Published in: ChemRxiv, 2020
Publisher: ChemRxiv
DOI: 10.26434/chemrxiv.13135769.v1

Simultaneous Evaluation of Multiple Microarray Surface Chemistries Through Real-Time Interferometric Imaging.

Author(s): Chiodi, Elisa; Sola, Laura; Brambilla, Dario; Cretich, Marina; Marn, Allison; Ünlü, M. Selim; et al.
Published in: Chemrxiv, 2019
Publisher: Chemrxiv
DOI: 10.26434/chemrxiv.8976347.v1

Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics

Author(s): Dario Brambilla; Laura Sola; Marcella Chiari
Published in: Chemrxiv, Issue 1, 2020
Publisher: Chemrxiv
DOI: 10.26434/chemrxiv.12227255.v1

Author Correction: Label-free detection of nanoparticles using depth scanning correlation interferometric microscopy

Author(s): Ugur Aygun, Hakan Urey, Ayca Yalcin Ozkumur
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-53131-3

Small evs-associated dna as complementary biomarker to circulating tumor dna in plasma of metastatic colorectal cancer patients

Author(s): Silvia Galbiati, Francesco Damin, Dario Brambilla, Lucia Ferraro, Nadia Soriani, Anna Maria Ferretti, Valentina Burgio, Monica Ronzoni, Riccardo Vago, Laura Sola. Marcella Chiari,
Published in: Pharmaceuticals, 2021, ISSN 1424-8247
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ph14020128

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols Towards Downstream Isolation and Analysis

Author(s): Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Published in: Cells 2021, Issue 10, 2021, Page(s) 544, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells10030544

High-Throughput, High-Resolution Interferometric Light Microscopy of Biological Nanoparticles

Author(s): Celalettin Yurdakul, Oguzhan Avci, Alex Matlock, Alexander J. Devaux, Maritza V. Quintero, Ekmel Ozbay, Robert A. Davey, John H. Connor, W. Clem Karl, Lei Tian, M. Selim Ünlü
Published in: ACS Nano, Issue 14/2, 2020, Page(s) 2002-2013, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.9b08512

The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Author(s): Elisa Chiodi, Allison M. Marn, Matthew T. Geib, M. Selim Ünlü
Published in: Polymers, Issue 13/7, 2021, Page(s) 1026, ISSN 2073-4360
Publisher: MDPI
DOI: 10.3390/polym13071026

Microarray Approach Combined with ddPCR: An Useful Pipeline for the Detection and Quantification of Circulating Tumour dna Mutations

Author(s): Galbiati, Damin, Ferraro, Soriani, Burgio, Ronzoni, Gianni, Ferrari, Chiari
Published in: Cells, Issue 8/8, 2019, Page(s) 769, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells8080769

Analysis of KRAS, NRAS and BRAF mutational profile by combination of in-tube hybridization and universal tag-microarray in tumor tissue and plasma of colorectal cancer patients

Author(s): Francesco Damin, Silvia Galbiati, Nadia Soriani, Valentina Burgio, Monica Ronzoni, Maurizio Ferrari, Marcella Chiari
Published in: PLOS ONE, Issue 13/12, 2018, Page(s) e0207876, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0207876

Membrane‐binding peptides for extracellular vesicles on‐chip analysis

Author(s): Alessandro Gori, Alessandro Romanato, Greta Bergamaschi, Alessandro Strada, Paola Gagni, Roberto Frigerio, Dario Brambilla, Riccardo Vago, Silvia Galbiati, Silvia Picciolini, Marzia Bedoni, George G. Daaboul, Marcella Chiari, Marina Cretich
Published in: Journal of Extracellular Vesicles, Issue 9/1, 2020, Page(s) 1751428, ISSN 2001-3078
Publisher: Taylor&Francis
DOI: 10.1080/20013078.2020.1751428

Computational Analysis of Dengue Virus Envelope Protein (E) Reveals an Epitope with Flavivirus Immunodiagnostic Potential in Peptide Microarrays

Author(s): Greta Bergamaschi, Enrico M. A. Fassi, Alessandro Romanato, Ilda D'Annessa, Maria Teresa Odinolfi, Dario Brambilla, Francesco Damin, Marcella Chiari, Alessandro Gori, Giorgio Colombo, Marina Cretich
Published in: International Journal of Molecular Sciences, Issue 20/8, 2019, Page(s) 1921, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20081921

A Reliable, Label Free Quality Control Method for the Production of DNA Microarrays with Clinical Applications

Author(s): Elisa Chiodi, Francesco Damin,*,Laura Sola*, Lucia Ferraro, Dario Brambilla, M. Selim Ünlü and Marcella Chiari
Published in: Polymers, 2021, ISSN 2073-4360
Publisher: MDPI
DOI: 10.3390/polym13030340

Interferometric Detection and Enumeration of Viral Particles using Si-Based Microfluidics

Author(s): Ayca Yalcin Ozkumur, Fulya Ekiz Kanik, Jacob T. Trueb, Celalettin Yurdakul, M. Selim Unlu
Published in: IEEE Journal of Selected Topics in Quantum Electronics, Issue 25/1, 2019, Page(s) 1-7, ISSN 1077-260X
Publisher: Institute of Electrical and Electronics Engineers
DOI: 10.1109/jstqe.2018.2854548

High-visibility detection of exosomes by interferometric reflectance imaging

Author(s): Unlu, Selim; Yurdakul, Celalettin; Yalcin-Ozkumur, Ayca; Chiari, Marcella; Ekiz-Kanik, Fulya; et al.
Published in: Journal of Extracellular Vesicles, 2018, Page(s) 248, ISSN 2001-3078
Publisher: Taylor&Francis

Intellectual Property Rights

METHOD FOR THE ISOLATION OF INTACT EXTRACELLULAR VESICLES

Application/Publication number: 20 200476
Date: 2020-10-07

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