Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX)

Risultati finali

Completion of benchmarking protocol and flowsheets established for the case studies
Stakeholder analysis
Identification of genes predicted to encode relevant robust BVMOs and oxidases
Public website and newsletter, first press releases on the project scopes
Overall analysis of environmental benefits and techno-economics of the processes and platform
Structural elucidation of relevant of BVMOs e.g. at least 3 new enzyme structures

Structural elucidation of relevant of BVMOs eg at least 3 new enzyme structures

Project leaflet in several languages
Communication and dissemination plan

Quorum sensing technology and micro fluidics platform available
Optimized enzyme production processes for ROBOX enzymes in bioreactor including DSP
Set of P450 enzymes with improved performance for hydroxylation of aromatics, alkenes and APIs and a set of API-sensors to enable P450 improvement
First generation production strains for robust BVMOs
First generation production strains for microbial and human P450s
96-well capillary electrophoreses screening system and first flow cytometer based screening system available
Generation of a first flow cytometer based screening system for quantification of one product in the BM3 demonstration process through fluorogenic sensors
New vector and host systems for enzyme coexpression
Biocatalytic and structural exploration of P450-AX as well as generation of improved variants

Installation of advisory boards

Pubblicazioni

Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters

Autori: Marie A. F. Delgove, Maximilian J. L. J. Fürst, Marco W. Fraaije, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Pubblicato in: ChemBioChem, 2017, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700427

Production of diclofenac metabolites by applying cytochrome P450 technology

Autori: Iwona Kaluzna, Jan Brummund, and Martin Schuermann
Pubblicato in: Chimica Oggi - Chemistry Today, Numero vol. 35(6) November/December 2017, 2017, Pagina/e 55-58, ISSN 0392-839X
Editore: Teknoscienze s.r.l.

Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution

Autori: Guangyue Li, Maximilian J. L. J. Fürst, Hamid Reza Mansouri, Anna K. Ressmann, Adriana Ilie, Florian Rudroff, Marko D. Mihovilovic, Marco W. Fraaije, Manfred T. Reetz
Pubblicato in: Organic & Biomolecular Chemistry, Numero 15/46, 2017, Pagina/e 9824-9829, ISSN 1477-0520
Editore: Royal Society of Chemistry
DOI: 10.1039/C7OB02692G

Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes

Autori: Elvira Romero, J. Rubén Gómez Castellanos, Giovanni Gadda, Marco W. Fraaije, Andrea Mattevi
Pubblicato in: Chemical Reviews, Numero 118/4, 2018, Pagina/e 1742-1769, ISSN 0009-2665
Editore: American Chemical Society
DOI: 10.1021/acs.chemrev.7b00650

Polycyclic Ketone Monooxygenase (PockeMO): A Robust Biocatalyst for the Synthesis of Optically Active Sulfoxides

Autori: Gonzalo de Gonzalo, Maximilian Fürst, Marco Fraaije
Pubblicato in: Catalysts, Numero 7/12, 2017, Pagina/e 288, ISSN 2073-4344
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal7100288

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions

Autori: Friso S. Aalbers, Marco W. Fraaije
Pubblicato in: Applied Microbiology and Biotechnology, Numero 101/20, 2017, Pagina/e 7557-7565, ISSN 0175-7598
Editore: Springer Verlag
DOI: 10.1007/s00253-017-8501-4

Application of a thermostable Baeyer-Villiger monooxygenase for the synthesis of branched polyester precursors

Autori: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M.A. De Wildeman
Pubblicato in: Journal of Chemical Technology & Biotechnology, 2018, ISSN 0268-2575
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5623

Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst

Autori: Quoc-Thai Nguyen, Gonzalo de Gonzalo, Claudia Binda, Ana Rioz-Martínez, Andrea Mattevi, Marco W. Fraaije
Pubblicato in: ChemBioChem, Numero 17/14, 2016, Pagina/e 1359-1366, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600148

Overriding Traditional Electronic Effects in Biocatalytic Baeyer–Villiger Reactions by Directed Evolution

Autori: Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk, Manfred T. Reetz
Pubblicato in: Journal of the American Chemical Society, Numero 140/33, 2018, Pagina/e 10464-10472, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.8b04742

Toward Upscaled Biocatalytic Preparation of Lactone Building Blocks for Polymer Applications

Autori: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Pubblicato in: Organic Process Research & Development, Numero 22/7, 2018, Pagina/e 803-812, ISSN 1083-6160
Editore: American Chemical Society
DOI: 10.1021/acs.oprd.8b00079

High performing immobilized Baeyer-Villiger monooxygenase and glucose dehydrogenase for the synthesis of Ɛ-caprolactone derivative

Autori: Marie A.F. Delgove, Daniela Valencia, Jordi Solé, Katrien V. Bernaerts, Stefaan M.A. De Wildeman, Marina Guillén, Gregorio Álvaro
Pubblicato in: Applied Catalysis A: General, Numero 572, 2018, Pagina/e 134-141, ISSN 0926-860X
Editore: Elsevier BV
DOI: 10.1016/j.apcata.2018.12.036

A prospective life-cycle assessment (LCA) of monomer synthesis: Comparison of biocatalytic and oxidative chemistry

Autori: Marie A. F. Delgove, Achille-B. Laurent, John M. Woodley, Stefaan M. A. De Wildeman, Katrien V. Bernaerts, Yvonne van der Meer
Pubblicato in: ChemSusChem, 2019, ISSN 1864-5631
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.201900007

A hydroquinone-specific screening system for directed P450 evolution

Autori: Alexandra M. Weingartner, Daniel F. Sauer, Gaurao V. Dhoke, Mehdi D. Davari, Anna Joëlle Ruff, Ulrich Schwaneberg
Pubblicato in: Applied Microbiology and Biotechnology, Numero 102/22, 2018, Pagina/e 9657-9667, ISSN 0175-7598
Editore: Springer Verlag
DOI: 10.1007/s00253-018-9328-3

Online Measurement of Oxygen-Dependent Enzyme Reaction Kinetics

Autori: Murray P. Meissner, Mathias Nordblad, John M. Woodley
Pubblicato in: ChemBioChem, Numero 19/1, 2018, Pagina/e 106-113, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700577

Scoping Biocatalyst Performance Using Reaction Trajectory Analysis

Autori: Mathias Nordblad, Mafalda Dias Gomes, Murray P. Meissner, Hemalata Ramesh, John M. Woodley
Pubblicato in: Organic Process Research & Development, Numero 22/9, 2018, Pagina/e 1101-1114, ISSN 1083-6160
Editore: American Chemical Society
DOI: 10.1021/acs.oprd.8b00119

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol

Autori: Quoc-Thai Nguyen, Elvira Romero, Willem P. Dijkman, Suzan Pantaroto de Vasconcellos, Claudia Binda, Andrea Mattevi, Marco W. Fraaije
Pubblicato in: Biochemistry, Numero 57/43, 2018, Pagina/e 6209-6218, ISSN 0006-2960
Editore: American Chemical Society
DOI: 10.1021/acs.biochem.8b00918

Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes

Autori: Friso S. Aalbers, Marco W. Fraaije
Pubblicato in: ChemBioChem, Numero 20/1, 2019, Pagina/e 20-28, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800394

Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases

Autori: Maximilian JLJ Fürst, Filippo Fiorentini, Marco W Fraaije
Pubblicato in: Current Opinion in Structural Biology, Numero 59, 2019, Pagina/e 29-37, ISSN 0959-440X
Editore: Elsevier BV
DOI: 10.1016/j.sbi.2019.01.019

Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase-NADPH Oxidase Fusions for Continuous Oxidations

Autori: Friso S. Aalbers, Marco W. Fraaije
Pubblicato in: ChemBioChem, Numero 20/1, 2019, Pagina/e 51-56, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800421

What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis

Autori: Ángela Mourelle-Insua, Friso S. Aalbers, Iván Lavandera, Vicente Gotor-Fernández, Marco W. Fraaije
Pubblicato in: Tetrahedron, Numero 75/13, 2019, Pagina/e 1832-1839, ISSN 0040-4020
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.tet.2019.02.015

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme

Autori: Maximilian J. L. J. Fürst, Elvira Romero, J. Rúben Gómez Castellanos, Marco W. Fraaije, Andrea Mattevi
Pubblicato in: ACS Catalysis, Numero 8/12, 2018, Pagina/e 11648-11656, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.8b03793

Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope

Autori: Joanne L. Porter, Selina Sabatini, Jack Manning, Michele Tavanti, James L. Galman, Nicholas J. Turner, Sabine L. Flitsch
Pubblicato in: Enzyme and Microbial Technology, Numero 113, 2018, Pagina/e 1-8, ISSN 0141-0229
Editore: Elsevier BV
DOI: 10.1016/j.enzmictec.2018.02.005

Characterisation of CYP102A25 from Bacillus marmarensis and CYP102A26 from Pontibacillus halophilus : P450 Homologues of BM3 with Preference towards Hydroxylation of Medium-Chain Fatty Acids

Autori: Joanne L. Porter, Jack Manning, Selina Sabatini, Michele Tavanti, Nicholas J. Turner, Sabine L. Flitsch
Pubblicato in: ChemBioChem, Numero 19/5, 2018, Pagina/e 513-520, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700598

A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Autori: Michele Tavanti, Juan Mangas-Sanchez, Sarah L. Montgomery, Matthew P. Thompson, Nicholas J. Turner
Pubblicato in: Organic & Biomolecular Chemistry, Numero 15/46, 2017, Pagina/e 9790-9793, ISSN 1477-0520
Editore: Royal Society of Chemistry
DOI: 10.1039/c7ob02569f

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Autori: Astrid Weninger, Jasmin E. Fischer, Hana Raschmanová, Claudia Kniely, Thomas Vogl, Anton Glieder
Pubblicato in: Journal of Cellular Biochemistry, Numero 119/4, 2018, Pagina/e 3183-3198, ISSN 0730-2312
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jcb.26474

Co-immobilization of P450 BM3 and glucose dehydrogenase on different supports for application as a self-sufficient oxidative biocatalyst

Autori: Jordi Solé, Gloria Caminal, Martin Schürmann, Gregorio Álvaro, Marina Guillén
Pubblicato in: Journal of Chemical Technology & Biotechnology, Numero 94/1, 2019, Pagina/e 244-255, ISSN 0268-2575
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5770

One-pot Biocatalytic Double Oxidation of α-Isophorone for the Synthesis of Ketoisophorone

Autori: Michele Tavanti, Fabio Parmeggiani, Jose Rubén Gómez Castellanos, Andrea Mattevi, Nicholas John Turner
Pubblicato in: ChemCatChem, 2017, ISSN 1867-3880
Editore: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201700620

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase

Autori: Elvira Romero, J. Rubén Gómez Castellanos, Andrea Mattevi, Marco W. Fraaije
Pubblicato in: Angewandte Chemie, Numero 128/51, 2016, Pagina/e 16084-16087, ISSN 0044-8249
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201608951

Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces thermophila : A Structurally Distinct Biocatalyst for Bulky Substrates

Autori: Maximilian J. L. J. Fürst, Simone Savino, Hanna M. Dudek, J. Rúben Gómez Castellanos, Cora Gutiérrez de Souza, Stefano Rovida, Marco W. Fraaije, Andrea Mattevi
Pubblicato in: Journal of the American Chemical Society, Numero 139/2, 2017, Pagina/e 627-630, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.6b12246

Increasing the solubility range of polyesters by tuning their microstructure with comonomers

Autori: Marie A. F. Delgove, Juandré Luchies, Iris Wauters, Geert G. P. Deroover, Stefaan M. A. De Wildeman, Katrien V. Bernaerts
Pubblicato in: Polym. Chem., 2017, ISSN 1759-9954
Editore: Royal Society of Chemistry
DOI: 10.1039/C7PY00976C

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins

Autori: Max J.L.J. Fürst, Caterina Martin, Nikola Lončar, Marco W. Fraaije
Pubblicato in: Methods in Enzymology, Numero 2018 June 9, 2018, Pagina/e 151-187, ISBN 978-0-12-815148-8
Editore: Elsevier
DOI: 10.1016/bs.mie.2018.04.007

Flavoenzymes at the crossroads of biocatalysis and structural biology

Autori: Nguyen, Quoc-Thai
Pubblicato in: Numero 1, 2017
Editore: Universityof Groningen

Diritti di proprietà intellettuale

KETOREDUCTASES

Numero candidatura/pubblicazione: WO 2019012095
Data: 2017-07-14

Risultati finali

Pubblicazioni

Diritti di proprietà intellettuale

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