Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX)

Resultado final

Completion of benchmarking protocol and flowsheets established for the case studies
Stakeholder analysis
Identification of genes predicted to encode relevant robust BVMOs and oxidases
Public website and newsletter, first press releases on the project scopes
Overall analysis of environmental benefits and techno-economics of the processes and platform
Structural elucidation of relevant of BVMOs e.g. at least 3 new enzyme structures

Structural elucidation of relevant of BVMOs eg at least 3 new enzyme structures

Project leaflet in several languages
Communication and dissemination plan

Quorum sensing technology and micro fluidics platform available
Optimized enzyme production processes for ROBOX enzymes in bioreactor including DSP
Set of P450 enzymes with improved performance for hydroxylation of aromatics, alkenes and APIs and a set of API-sensors to enable P450 improvement
First generation production strains for robust BVMOs
First generation production strains for microbial and human P450s
96-well capillary electrophoreses screening system and first flow cytometer based screening system available
Generation of a first flow cytometer based screening system for quantification of one product in the BM3 demonstration process through fluorogenic sensors
New vector and host systems for enzyme coexpression
Biocatalytic and structural exploration of P450-AX as well as generation of improved variants

Installation of advisory boards

Publicaciones

Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters

Autores: Marie A. F. Delgove, Maximilian J. L. J. Fürst, Marco W. Fraaije, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Publicado en: ChemBioChem, 2017, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700427

Production of diclofenac metabolites by applying cytochrome P450 technology

Autores: Iwona Kaluzna, Jan Brummund, and Martin Schuermann
Publicado en: Chimica Oggi - Chemistry Today, Edición vol. 35(6) November/December 2017, 2017, Página(s) 55-58, ISSN 0392-839X
Editor: Teknoscienze s.r.l.

Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution

Autores: Guangyue Li, Maximilian J. L. J. Fürst, Hamid Reza Mansouri, Anna K. Ressmann, Adriana Ilie, Florian Rudroff, Marko D. Mihovilovic, Marco W. Fraaije, Manfred T. Reetz
Publicado en: Organic & Biomolecular Chemistry, Edición 15/46, 2017, Página(s) 9824-9829, ISSN 1477-0520
Editor: Royal Society of Chemistry
DOI: 10.1039/C7OB02692G

Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes

Autores: Elvira Romero, J. Rubén Gómez Castellanos, Giovanni Gadda, Marco W. Fraaije, Andrea Mattevi
Publicado en: Chemical Reviews, Edición 118/4, 2018, Página(s) 1742-1769, ISSN 0009-2665
Editor: American Chemical Society
DOI: 10.1021/acs.chemrev.7b00650

Polycyclic Ketone Monooxygenase (PockeMO): A Robust Biocatalyst for the Synthesis of Optically Active Sulfoxides

Autores: Gonzalo de Gonzalo, Maximilian Fürst, Marco Fraaije
Publicado en: Catalysts, Edición 7/12, 2017, Página(s) 288, ISSN 2073-4344
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal7100288

Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions

Autores: Friso S. Aalbers, Marco W. Fraaije
Publicado en: Applied Microbiology and Biotechnology, Edición 101/20, 2017, Página(s) 7557-7565, ISSN 0175-7598
Editor: Springer Verlag
DOI: 10.1007/s00253-017-8501-4

Application of a thermostable Baeyer-Villiger monooxygenase for the synthesis of branched polyester precursors

Autores: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M.A. De Wildeman
Publicado en: Journal of Chemical Technology & Biotechnology, 2018, ISSN 0268-2575
Editor: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5623

Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst

Autores: Quoc-Thai Nguyen, Gonzalo de Gonzalo, Claudia Binda, Ana Rioz-Martínez, Andrea Mattevi, Marco W. Fraaije
Publicado en: ChemBioChem, Edición 17/14, 2016, Página(s) 1359-1366, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600148

Overriding Traditional Electronic Effects in Biocatalytic Baeyer–Villiger Reactions by Directed Evolution

Autores: Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk, Manfred T. Reetz
Publicado en: Journal of the American Chemical Society, Edición 140/33, 2018, Página(s) 10464-10472, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.8b04742

Toward Upscaled Biocatalytic Preparation of Lactone Building Blocks for Polymer Applications

Autores: Marie A. F. Delgove, Matthew T. Elford, Katrien V. Bernaerts, Stefaan M. A. De Wildeman
Publicado en: Organic Process Research & Development, Edición 22/7, 2018, Página(s) 803-812, ISSN 1083-6160
Editor: American Chemical Society
DOI: 10.1021/acs.oprd.8b00079

High performing immobilized Baeyer-Villiger monooxygenase and glucose dehydrogenase for the synthesis of Ɛ-caprolactone derivative

Autores: Marie A.F. Delgove, Daniela Valencia, Jordi Solé, Katrien V. Bernaerts, Stefaan M.A. De Wildeman, Marina Guillén, Gregorio Álvaro
Publicado en: Applied Catalysis A: General, Edición 572, 2018, Página(s) 134-141, ISSN 0926-860X
Editor: Elsevier BV
DOI: 10.1016/j.apcata.2018.12.036

A prospective life-cycle assessment (LCA) of monomer synthesis: Comparison of biocatalytic and oxidative chemistry

Autores: Marie A. F. Delgove, Achille-B. Laurent, John M. Woodley, Stefaan M. A. De Wildeman, Katrien V. Bernaerts, Yvonne van der Meer
Publicado en: ChemSusChem, 2019, ISSN 1864-5631
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.201900007

A hydroquinone-specific screening system for directed P450 evolution

Autores: Alexandra M. Weingartner, Daniel F. Sauer, Gaurao V. Dhoke, Mehdi D. Davari, Anna Joëlle Ruff, Ulrich Schwaneberg
Publicado en: Applied Microbiology and Biotechnology, Edición 102/22, 2018, Página(s) 9657-9667, ISSN 0175-7598
Editor: Springer Verlag
DOI: 10.1007/s00253-018-9328-3

Online Measurement of Oxygen-Dependent Enzyme Reaction Kinetics

Autores: Murray P. Meissner, Mathias Nordblad, John M. Woodley
Publicado en: ChemBioChem, Edición 19/1, 2018, Página(s) 106-113, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700577

Scoping Biocatalyst Performance Using Reaction Trajectory Analysis

Autores: Mathias Nordblad, Mafalda Dias Gomes, Murray P. Meissner, Hemalata Ramesh, John M. Woodley
Publicado en: Organic Process Research & Development, Edición 22/9, 2018, Página(s) 1101-1114, ISSN 1083-6160
Editor: American Chemical Society
DOI: 10.1021/acs.oprd.8b00119

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol

Autores: Quoc-Thai Nguyen, Elvira Romero, Willem P. Dijkman, Suzan Pantaroto de Vasconcellos, Claudia Binda, Andrea Mattevi, Marco W. Fraaije
Publicado en: Biochemistry, Edición 57/43, 2018, Página(s) 6209-6218, ISSN 0006-2960
Editor: American Chemical Society
DOI: 10.1021/acs.biochem.8b00918

Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes

Autores: Friso S. Aalbers, Marco W. Fraaije
Publicado en: ChemBioChem, Edición 20/1, 2019, Página(s) 20-28, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800394

Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases

Autores: Maximilian JLJ Fürst, Filippo Fiorentini, Marco W Fraaije
Publicado en: Current Opinion in Structural Biology, Edición 59, 2019, Página(s) 29-37, ISSN 0959-440X
Editor: Elsevier BV
DOI: 10.1016/j.sbi.2019.01.019

Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase-NADPH Oxidase Fusions for Continuous Oxidations

Autores: Friso S. Aalbers, Marco W. Fraaije
Publicado en: ChemBioChem, Edición 20/1, 2019, Página(s) 51-56, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800421

What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis

Autores: Ángela Mourelle-Insua, Friso S. Aalbers, Iván Lavandera, Vicente Gotor-Fernández, Marco W. Fraaije
Publicado en: Tetrahedron, Edición 75/13, 2019, Página(s) 1832-1839, ISSN 0040-4020
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.tet.2019.02.015

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme

Autores: Maximilian J. L. J. Fürst, Elvira Romero, J. Rúben Gómez Castellanos, Marco W. Fraaije, Andrea Mattevi
Publicado en: ACS Catalysis, Edición 8/12, 2018, Página(s) 11648-11656, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.8b03793

Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope

Autores: Joanne L. Porter, Selina Sabatini, Jack Manning, Michele Tavanti, James L. Galman, Nicholas J. Turner, Sabine L. Flitsch
Publicado en: Enzyme and Microbial Technology, Edición 113, 2018, Página(s) 1-8, ISSN 0141-0229
Editor: Elsevier BV
DOI: 10.1016/j.enzmictec.2018.02.005

Characterisation of CYP102A25 from Bacillus marmarensis and CYP102A26 from Pontibacillus halophilus : P450 Homologues of BM3 with Preference towards Hydroxylation of Medium-Chain Fatty Acids

Autores: Joanne L. Porter, Jack Manning, Selina Sabatini, Michele Tavanti, Nicholas J. Turner, Sabine L. Flitsch
Publicado en: ChemBioChem, Edición 19/5, 2018, Página(s) 513-520, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700598

A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Autores: Michele Tavanti, Juan Mangas-Sanchez, Sarah L. Montgomery, Matthew P. Thompson, Nicholas J. Turner
Publicado en: Organic & Biomolecular Chemistry, Edición 15/46, 2017, Página(s) 9790-9793, ISSN 1477-0520
Editor: Royal Society of Chemistry
DOI: 10.1039/c7ob02569f

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Autores: Astrid Weninger, Jasmin E. Fischer, Hana Raschmanová, Claudia Kniely, Thomas Vogl, Anton Glieder
Publicado en: Journal of Cellular Biochemistry, Edición 119/4, 2018, Página(s) 3183-3198, ISSN 0730-2312
Editor: John Wiley & Sons Inc.
DOI: 10.1002/jcb.26474

Co-immobilization of P450 BM3 and glucose dehydrogenase on different supports for application as a self-sufficient oxidative biocatalyst

Autores: Jordi Solé, Gloria Caminal, Martin Schürmann, Gregorio Álvaro, Marina Guillén
Publicado en: Journal of Chemical Technology & Biotechnology, Edición 94/1, 2019, Página(s) 244-255, ISSN 0268-2575
Editor: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5770

One-pot Biocatalytic Double Oxidation of α-Isophorone for the Synthesis of Ketoisophorone

Autores: Michele Tavanti, Fabio Parmeggiani, Jose Rubén Gómez Castellanos, Andrea Mattevi, Nicholas John Turner
Publicado en: ChemCatChem, 2017, ISSN 1867-3880
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201700620

Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase

Autores: Elvira Romero, J. Rubén Gómez Castellanos, Andrea Mattevi, Marco W. Fraaije
Publicado en: Angewandte Chemie, Edición 128/51, 2016, Página(s) 16084-16087, ISSN 0044-8249
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201608951

Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces thermophila : A Structurally Distinct Biocatalyst for Bulky Substrates

Autores: Maximilian J. L. J. Fürst, Simone Savino, Hanna M. Dudek, J. Rúben Gómez Castellanos, Cora Gutiérrez de Souza, Stefano Rovida, Marco W. Fraaije, Andrea Mattevi
Publicado en: Journal of the American Chemical Society, Edición 139/2, 2017, Página(s) 627-630, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.6b12246

Increasing the solubility range of polyesters by tuning their microstructure with comonomers

Autores: Marie A. F. Delgove, Juandré Luchies, Iris Wauters, Geert G. P. Deroover, Stefaan M. A. De Wildeman, Katrien V. Bernaerts
Publicado en: Polym. Chem., 2017, ISSN 1759-9954
Editor: Royal Society of Chemistry
DOI: 10.1039/C7PY00976C

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins

Autores: Max J.L.J. Fürst, Caterina Martin, Nikola Lončar, Marco W. Fraaije
Publicado en: Methods in Enzymology, Edición 2018 June 9, 2018, Página(s) 151-187, ISBN 978-0-12-815148-8
Editor: Elsevier
DOI: 10.1016/bs.mie.2018.04.007

Flavoenzymes at the crossroads of biocatalysis and structural biology

Autores: Nguyen, Quoc-Thai
Publicado en: Edición 1, 2017
Editor: Universityof Groningen

Derechos de propiedad intelectual

KETOREDUCTASES

Número de solicitud/publicación: WO 2019012095
Fecha: 2017-07-14

Resultado final

Publicaciones

Derechos de propiedad intelectual

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