A microsystem that integrates the modules of extraction, metering, dilution, reagent delivery and detection has been demonstrated as a laboratory prototype. The individual micromodules have been realised in single polymer chips. To obtain a complete system these chips have to be assembled on a fluidic platform. The chip-based-lab platform developed at IMM was specially designed for this purpose. A metal plate with metal frames can house the fluidic chips in any desired combination. Interfacing chips are used to get the fluidic contact between the chips. Active flow control components have been described in previous abstracts.
These are:
- A syringe pump. This pump is connected to several inlet ports addressed by external valves to avoid a too deep pressure drop. This could be obtained if all chips would be addressed by only one single port.
- Light barriers that monitor the arrival of fluids at specific spots of the chip to indicate the right time to switch valves.
- Valves. Internal valves follow one single principle. Different versions vary only by the design of the slits in the rubber disk. All valves are actuated by electric stepper motors. Their position is monitored by common forked light barriers.
All components are controlled by an electronic compartment which was designed also in a modular way reflecting the same flexibility that was gained on the fluidic side with the chip-based-lab platform and its standardised fluidic chips.
The assembly of a complete system was realised with different aspects.
-·Automatic switching of the valves
-·Automatic monitoring of the plug position
-·Automatic operation of the extraction motor
-·Automatic operation of the centrifuge including centrifugation and the filling process
-·Automatic detection signal read out
-·Interface to a fluidic actuation system with pumps and external valves
-·Control of the progress by an electronic system
The functionality of single fluidic chips has been tested successfully. In the actual state, combinations of several chips were tested. In detail this has been
-·Extraction and centrifuge
-·Dilution and mixing
-·Dilution, mixing and distribution
-·Metering and distribution
-·Metering, distribution, ELISA and detection
-·Storage (ELISA), Metering, distribution, ELISA and detection
Since the realisation of a functional dilution chip was achieved very late at the end of the project, as well as the complete actuation, monitoring and electronic control, only first tests of the complete system were performed. Primary results showed that the system is working in principle but that there is extra effort needed to obtain a robust system for the measurement of real samples.