Risultati finali
Protocol for establishing the gut-epithelial model in STACKS [M6]. A standard protocol for the transfer of gut epithelial monolayer model from Transwell to STACKS will be provided. [R] [PU]
Manuscript on the effect of fibroblasts on gut epithelial differentiation [M24]. The role of fibroblasts within the Biosilk network to effect gut differentiation in vitro will be elucidated. [R] [PU]
Protocol for cytotoxicity assay in gut mucosa model [M30] [Report on the best method for cytotoxicity assessment] [R] [PU]
Protocol for iPSC-derived BBB model [M24] [A standard protocol for establishing a BBB model will be provided]. [R] [PU]
Manuscript on the method to co-culture gut-epithelial model with immune cells [M24]. [R] [PU]
Report on the properties of the Biosilk network [M24]. The complete properties of the Biosilk network such as tensile and cell motility will be characterised. [R] [PU]
Characterisation panel for iPSC-derived BBB model. [M24] [Report on standard range of parameters for BBB model] [R] [PU]
Report on the properties of the Biosilk nanomembrane [M24]. The complete properties of the Biosilk nanomembrane such as porosity and mass transport will be characterised. [R] [PU]
Data Management Plan [M6, M30, M48] Regular updates of DMP is uploaded to the website. [R] [PU]
Protocol for establishing the gutepithelial model in STACKS M6 A standard protocol for the transfer of gut epithelial monolayer model from Transwell to STACKS will be provided R PU
Report on diffusion properties through the Biosilk matrix [M12]. Diffusion properties of fluorochromes in the Biosilk network with and without cells will be reported. [R] [PU]Report on diffusion properties through the Biosilk matrix M12 Diffusion properties of fluorochromes in the Biosilk network with and without cells will be reported R PU
Definition of standard values for specific immune response parameters in the gut epithelial-immune cell co-culture model [M24]. A report on the measured standard values for immune markers will be available. [R] [PU]Manuscript on the effect of fibroblasts on gut epithelial differentiation [M24]. The role of fibroblasts within the Biosilk network to effect gut differentiation in vitro will be elucidated. [R] [PU]
Protocol for cytotoxicity assay in gut mucosa model [M30] [Report on the best method for cytotoxicity assessment] [R] [PU]
Protocol for iPSC-derived BBB model [M24] [A standard protocol for establishing a BBB model will be provided]. [R] [PU]
Manuscript on the method to co-culture gut-epithelial model with immune cells [M24]. [R] [PU]
Report on the properties of the Biosilk network [M24]. The complete properties of the Biosilk network such as tensile and cell motility will be characterised. [R] [PU]
Characterisation panel for iPSC-derived BBB model. [M24] [Report on standard range of parameters for BBB model] [R] [PU]
Report on the properties of the Biosilk nanomembrane [M24]. The complete properties of the Biosilk nanomembrane such as porosity and mass transport will be characterised. [R] [PU]
Data Management Plan [M6, M30, M48] Regular updates of DMP is uploaded to the website. [R] [PU]
Data Management Plan M6 M30 M48 Regular updates of DMP is uploaded to the website R PU
"Protocol for patterning ""crypt-villi"" features on Biosilk [M9]. A standard protocol for embossing or casting ""crypt-villi"" structures will be delivered. [R] [PU]"Protocol for patterning cryptvilli features on Biosilk M9 A standard protocol for embossing or casting cryptvilli structures will be delivered R PU
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