Description du projet
Un système complexe d’organe sur puce pour la recherche biomédicale
La technologie organoïde a réalisé une percée dans le domaine de la biomédecine et revêt un grand potentiel pour la pathogenèse virale et la recherche antivirale. Le projet GUTVIBRATIONS, financé par l’UE, combinera l’expertise dans la microfabrication, la technologie organoïde, la virologie, l’immunologie, et le génie biologique pour créer un système d’organe sur puce en tant que modèle modulaire à plusieurs organes de l’axe intestin-cerveau humain. Ce système sera conçu en combinant la technologie des échafaudages imprimés en 3D et des organoïdes intestinaux et cérébraux humains. Il comprendra une couche épithéliale de l’intestin, des cellules immunitaires, une barrière hémato-encéphalique et un organoïde cérébral, connectés par empilement vertical. L’échafaudage de la matrice extracellulaire (MEC) appelé Biosilk, une protéine recombinante de soie d’araignée, permettra de créer une niche microenvironnementale de type «in vivo». Le projet entend évaluer la commercialisation du système par une analyse du marché et un plan d’affaires.
Objectif
The battle against infectious diseases is hampered by lack of therapeutic innovations due to poor understanding of disease outcome in humans. Organoid technology is a major breakthrough for medical research that has traditionally relied heavily on animal models. In our current OrganoVIR consortium, we are at the forefront in establishing human organoids as superior models for viral pathogenesis and antiviral research. The next critical step is to integrate organoid models to create a complex multi-organ ex vivo model that better mimics the human physiology. This raises several challenges such as incorporation of various cell types, medium incompatibility, validation, high throughput, and robust ways to connect mature organoid models. At the same time, the model has to be accessible, user friendly, and affordable to all end users. Therefore, this proposed GUTVIBRATIONS consortium will leverage its expertise in microfabrication, organoid technology, virology, immunology, bioengineering, and materials science to deliver an enabling organ-on-chip system.
As a demonstrator, a modular human gut-brain axis multi-organ model will be achieved for studying viral disease and treatment. This model will be built by combining an open source 3D printed scaffold technology with human gut and brain organoid models. The deliverables will include a complex gut epithelial layer, immune cell layer, blood-brain layer and brain organoid; all individually developed and subsequently connected by vertical stacking. Our unique modular approach where individual components are separately developed and validated minimizes compatibility issues. The individual models and the multi-organ model will be used for studying three different clinically relevant viral infections with high global burden and will be validated against existing clinical data to bring the system to TRL5. Routes towards commercialization will also be assessed by performing a market analysis and developing a business plan.
Champ scientifique
- natural sciencesbiological sciencesmicrobiologyvirology
- medical and health scienceshealth sciencesinfectious diseases
- medical and health sciencesbasic medicineimmunology
- medical and health sciencesbasic medicinephysiology
- engineering and technologyother engineering and technologiesmicrotechnologyorgan on a chip
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Régime de financement
RIA - Research and Innovation actionCoordinateur
1081 HV Amsterdam
Pays-Bas