Descripción del proyecto
Un sistema complejo de órgano en chip para la investigación biomédica
La tecnología de los organoides ha logrado un gran avance en biomedicina con un potencial elevado para la investigación antivírica y sobre la patogénesis vírica. El proyecto GUTVIBRATIONS, financiado con fondos europeos, combinará conocimientos en microfabricación, tecnología de los organoides, virología, inmunología y bioingeniería para crear un sistema de órgano en chip como un modelo modular multiorgánico del eje intestino-encéfalo humano. Este sistema se creará combinando la tecnología de andamios impresos tridimensionales con organoides encefálicos e intestinales humanos. Incluirá una capa epitelial intestinal, células inmunitarias, una barrera hematoencefálica y un organoide encefálico conectados por apilamiento vertical. El andamio de matriz extracelular (MEC) llamado Biosilk, una proteína recombinante de la seda de araña, dará como resultado un nicho microambiental similar a una situación «in vivo». El proyecto pretende evaluar la comercialización del sistema con un análisis de mercado y un plan de negocio.
Objetivo
The battle against infectious diseases is hampered by lack of therapeutic innovations due to poor understanding of disease outcome in humans. Organoid technology is a major breakthrough for medical research that has traditionally relied heavily on animal models. In our current OrganoVIR consortium, we are at the forefront in establishing human organoids as superior models for viral pathogenesis and antiviral research. The next critical step is to integrate organoid models to create a complex multi-organ ex vivo model that better mimics the human physiology. This raises several challenges such as incorporation of various cell types, medium incompatibility, validation, high throughput, and robust ways to connect mature organoid models. At the same time, the model has to be accessible, user friendly, and affordable to all end users. Therefore, this proposed GUTVIBRATIONS consortium will leverage its expertise in microfabrication, organoid technology, virology, immunology, bioengineering, and materials science to deliver an enabling organ-on-chip system.
As a demonstrator, a modular human gut-brain axis multi-organ model will be achieved for studying viral disease and treatment. This model will be built by combining an open source 3D printed scaffold technology with human gut and brain organoid models. The deliverables will include a complex gut epithelial layer, immune cell layer, blood-brain layer and brain organoid; all individually developed and subsequently connected by vertical stacking. Our unique modular approach where individual components are separately developed and validated minimizes compatibility issues. The individual models and the multi-organ model will be used for studying three different clinically relevant viral infections with high global burden and will be validated against existing clinical data to bring the system to TRL5. Routes towards commercialization will also be assessed by performing a market analysis and developing a business plan.
Ámbito científico
- natural sciencesbiological sciencesmicrobiologyvirology
- medical and health scienceshealth sciencesinfectious diseases
- medical and health sciencesbasic medicineimmunology
- medical and health sciencesbasic medicinephysiology
- engineering and technologyother engineering and technologiesmicrotechnologyorgan on a chip
Palabras clave
Programa(s)
Convocatoria de propuestas
Consulte otros proyectos de esta convocatoriaConvocatoria de subcontratación
H2020-NMBP-TR-IND-2020-twostage
Régimen de financiación
RIA - Research and Innovation actionCoordinador
1081 HV Amsterdam
Países Bajos