General-purpose virus-neutralizing engulfing shells with modular target-specificity

Livrables

Public website: the website will present the partners, the overall research goals and objectives and regular updates about achievements (publications, patents), ongoing events, symposium and related activities from other entities around the world that are of interest.

VIROFIGHT communication kit

VIROFIGHT communication kit: This deliverable covers project flyer, slide show presentation (powerpoint, keynote) and poster.

Report on polymerized nucleic acids as engulfing shells on viruses

Report on polymerized nucleic acids as engulfing shells on virusesTriangular subunits for prefabricated shells furnished with virusspecific aptamers such that shell assembly will be templated by a virus surface AU will design small nucleic acid tiles featuring multiple aptamers that bind a repetitive subunit in the viral surface to yield 24 multivalent 46 nm shell building blocks with sticky DNA linkers stabilized into duplexes by the avidity effect from the viral surface CryoEM and electrophoretic mobility shift assays will be used to assess particle shape integrity and proper basic functionality Designs will be iteratively improved Report will cover results of these actions

Generation of non-infectious and infectious virus particles from non-enveloped viruses

Generation of noninfectious and infectious virus particles from nonenveloped virusesThis deliverable comprises solutions containing virus particles to be handed over to partners of the consortium for further experiments

Report on library of prototype DNA origami shell designs

Report on library of prototype DNA origami shell designs: This report will contain blueprints, 3D models, and DNA sequences needed to build the shells design variants. In addition, wherever applicable, experimental data obtained on the designs will be added and discussed, comprising e.g. cryo EM images, electrophoretic mobility shift analysis. etc

Generation of VLPs and LVs pseudotyped with surface proteins of enveloped viruses

Generation of VLPs and LVs pseudotyped with surface proteins of enveloped viruses: This deliverable comprises solutions containing the virus-like particles and lentiviral pseudotyped particles to be handed over to partners of the consortium for further experiments.

Report on assembly procedures for prefabricated DNA origami shells

Report on assembly procedures for prefabricated DNA origami shellsIn this report experimental procedures will be described leading to successful assembly of prefabricated DNA origami shells including thermal annealing conditions reaction mixture conditions purification steps and quality control procedures In addition troubleshooting measures and inluence of environmental conditions such as temperature or cation content on assembly will be described

Dissemination and communication plan

Dissemination and communication planA detailed communication and dissemination plan covering all communication and dissemination activities required during the project and adapted to the different target audiences and objectives of the communication or dissemination measures

Generation of hepatitis B viruses as exemplary enveloped virus

Generation of hepatitis B viruses as exemplary enveloped virusThis deliverable comprises solutions containing the hepatitis B viruses to be handed over to partners of the consortium for further experiments

Exploitation Innovation Plan

An initial exploitation innovation plan will be presented in M12 An updated version will be created in M36

Design details and protocols for modular swap out of virus recognizing moieties

Design details and protocols for modular swap out of virus recognizing moietiesSubsets of shellforming DNA oligonucleotides will be modified with terminal extensions pointing into the shell interior The sequences of the extensions will first be dummies to deal with and remove potential aggregation issues induced through Velcro effects Iterative refinements will be made so that arbitrary sequences can be attached in tunable decoration density and geometry in a plug and play fashion without affecting the shell quality and functionality AU will deliver virusbinding candidate DNA aptamer sequences to be included as direct terminal extensions or sticky linkers

Data Management Plan

Publications

Computationally designed Spike antigens induce neutralising responses against the breadth of SARS-COV-2 variants

Auteurs: Sneha Vishwanath, George William Carnell, Martina Billmeier, Luis Ohlendorf, Patrick Neckermann, Benedikt Asbach, Charlotte George, Maria Suau Sans, Andrew Chan, Joey Olivier, Angalee Nadesalingam, Sebastian Einhauser, Nigel Temperton, Diego Cantoni, Joe Grove, Ingo Jordan, Volker Sandig, Paul Tonks, Johannes Geiger, Christian Dohmen, Verena Mummert, Anne Rosalind Samuel, Christian Plank, Rebecca
Publié dans: npj Vaccines, Numéro 9, 2024, ISSN 2059-0105
Éditeur: Nature portfolio
DOI: 10.1038/s41541-024-00950-9

Comparable cellular and humoral immunity upon homologous and heterologous COVID-19 vaccination regimens in kidney transplant recipients

Auteurs: Nina Körber, Christopher Holzmann-Littig, Gesa Wilkens, Bo-Hung Liao, Maia L. Werz, Louise Platen, Cho-Chin Cheng, Myriam Tellenbach, Verena Kappler, Viktor Lehner, Hrvoje Mijočević, Catharina Christa, Volker Assfalg, Uwe Heemann, Christoph Schmaderer, Ulrike Protzer, Matthias C. Braunisch, Tanja Bauer, Lutz Renders
Publié dans: Frontiers in Immunology, Numéro 14, 2023, ISSN 1664-3224
Éditeur: Frontiers
DOI: 10.3389/fimmu.2023.1172477

Triple-Stranded DNA As a Structural Element in DNA Origami

Auteurs: Ken Sachenbacher, Ali Khoshouei, Maximilian Nicolas Honemann, Wouter Engelen, Elija Feigl, Hendrik Dietz
Publié dans: ACS Nano, Numéro 17, 2023, Page(s) 9014-9024, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.2c11402

Active Nuclear Import of Mammalian Cell-Expressible DNA Origami

Auteurs: Anna Liedl, Johanna Grießing, Jessica A. Kretzmann, Hendrik Dietz
Publié dans: Journal of the American Chemical Society, Numéro 145, 2023, Page(s) 4946-4950, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.2c12733

Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection

Auteurs: Del Rosario JMM, da Costa KAS, Asbach B, Ferrara F, Ferrari M, Wells DA, Mann GS, Ameh VO, Sabeta CT, Banyard AC, Kinsley R, Scott SD, Wagner R, Heeney JL, Carnell GW, Temperton NJ.
Publié dans: Vaccines, 2021, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9070741

Gene-encoding DNA origami for mammalian cell expression

Auteurs: Jessica A. Kretzmann, Anna Liedl, Alba Monferrer, Volodymyr Mykhailiuk, Samuel Beerkens, Hendrik Dietz
Publié dans: Nature Communications, Numéro 14, 2023, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-023-36601-1

Influenza A (N1-N9) and Influenza B (B/Victoria and B/Yamagata) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine Design

Auteurs: da Costa KAS, Del Rosario JMM, Ferrari M, Vishwanath S, Asbach B, Kinsley R, Wagner R, Heeney JL, Carnell GW, Temperton NJ.
Publié dans: Vaccines, Numéro 2022 Sep 14;10(9), 2022, Page(s) 1520, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines10091520

Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses

Auteurs: Sampson AT, Heeney J, Cantoni D, Ferrari M, Sans MS, George C, Di Genova C, Mayora Neto M, Einhauser S, Asbach B, Wagner R, Baxendale H, Temperton N, Carnell G.
Publié dans: Viruses (Basel), 2021, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13081579

A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.

Auteurs: Valero J, Civit L, Dupont DM, Selnihhin D, Reinert LS, Idorn M, Israels BA, Bednarz AM, Bus C, Asbach B, Peterhoff D, Pedersen FS, Birkedal V, Wagner R, Paludan SR, Kjems J.
Publié dans: Proc Natl Acad Sci, Numéro 2021 Dec 14;118(50), 2021, ISSN 1091-6490
Éditeur: National Academy of Science
DOI: 10.1073/pnas.2112942118

SARS‐CoV‐2 Productively Infects Human Hepatocytes and Induces Cell Death

Auteurs: Chunkyu Ko, Cho‐Chin Cheng, Daniele Mistretta, Shubhankar Ambike, Julia Sacherl, Stoyan Velkov, Bo‐Hung Liao, Romina Bester, Merve Gültan, Olga Polezhaeva, Alexander Herrmann, Constanze A. Jakwerth, Carsten B. Schmidt‐Weber, Joachim J. Bugert, Roman Wölfel, Vincent Grass, Sandra Essbauer, Daniel Schnepf, Oliver T. Keppler, Florian W. R. Vondran, Andreas Pichlmair, Carolin Mogler, Gregor Eb
Publié dans: Journal of Medical Virology, Numéro 97, 2025, ISSN 0146-6615
Éditeur: John Wiley & Sons Inc.
DOI: 10.1002/jmv.70156

SARS-CoV-2 Neutralization Capacity in Hemodialysis Patients with and without a Fifth Vaccination with the Updated Comirnaty Original/Omicron BA.4-5 Vaccine

Auteurs: Bo-Hung Liao, Louise Platen, Myriam Grommes, Cho-Chin Cheng, Christopher Holzmann-Littig, Catharina Christa, Bernhard Haller, Verena Kappler, Romina Bester, Maia Lucia Werz, Eva Platen, Peter Eggerer, Laëtitia Tréguer, Claudius Küchle, Christoph Schmaderer, Uwe Heemann, Lutz Renders, Ulrike Protzer, Matthias Christoph Braunisch
Publié dans: Vaccines, Numéro 12, 2025, Page(s) 308, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines12030308

Programmable icosahedral shell system for virus trapping

Auteurs: Christian Sigl, Elena M. Willner, Wouter Engelen, Jessica A. Kretzmann, Ken Sachenbacher, Anna Liedl, Fenna Kolbe, Florian Wilsch, S. Ali Aghvami, Ulrike Protzer, Michael F. Hagan, Seth Fraden, Hendrik Dietz
Publié dans: Nature Materials, 2021, ISSN 1476-1122
Éditeur: Nature Publishing Group
DOI: 10.1038/s41563-021-01020-4

A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

Auteurs: Laia Civit, Nima Moradzadeh, Anna Jonczyk, Patrick Neckermann, Benedikt Asbach, David Peterhoff, Ralf Wagner, Michael Famulok, Günter Mayer, Jørgen Kjems, Julián Valero
Publié dans: International Journal of Molecular Sciences, Numéro 25, 2025, Page(s) 4642, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms25094642

Preorganized cyclic modules facilitate the self-assembly of protein nanostructures

Auteurs: Jaka Snoj, Fabio Lapenta, Roman Jerala
Publié dans: Chemical Science, Numéro 15, 2024, Page(s) 3673-3686, ISSN 2041-6520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d3sc06658d

Beyond Dimerization: Harnessing Tetrameric Coiled‐Coils for Nanostructure Assembly

Auteurs: Sara Vidmar, Tamara Šmidlehner, Jana Aupič, Žiga Strmšek, Ajasja Ljubetič, Fei Xiao, Guang Hu, Chuan Liu, Florian Beck, Philipp S. Erdmann, Roman Jerala
Publié dans: Angewandte Chemie International Edition, 2024, ISSN 1433-7851
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202422075

Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein.

Auteurs: Staufer O, Gupta K, Hernandez Bücher JE, Kohler F, Sigl C, Singh G, Vasileiou K, Yagüe Relimpio A, Macher M, Fabritz S, Dietz H, Cavalcanti Adam EA, Schaffitzel C, Ruggieri A, Platzman I, Berger I, Spatz JP
Publié dans: Nat Commun, Numéro 2022 Feb 14;13(1), 2022, Page(s) 868, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-022-28446-x

Coupling of Spectrin Repeat Modules for the Assembly of Nanorods and Presentation of Protein Domains

Auteurs: Klemen Mezgec, Jaka Snoj, Liza Ulčakar, Ajasja Ljubetič, Magda Tušek Žnidarič, Miha Škarabot, Roman Jerala
Publié dans: ACS Nano, Numéro 18, 2024, Page(s) 28748-28763, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.4c07701

The art of designed coiled-coils for the regulation of mammalian cells

Auteurs: Tjaša Plaper, Erik Rihtar, Taja Železnik Ramuta, Vida Forstnerič, Vid Jazbec, Filip Ivanovski, Mojca Benčina, Roman Jerala
Publié dans: Cell Chemical Biology, Numéro 31, 2024, Page(s) 1460-1472, ISSN 2451-9448
Éditeur: Elsevier
DOI: 10.1016/j.chembiol.2024.06.001

DNA origami traps for large viruses

Auteurs: Alba Monferrer, Fabian Kohler, Christian Sigl, Michael Schachtner, David Peterhoff, Benedikt Asbach, Ralf Wagner, Hendrik Dietz
Publié dans: Cell Reports Physical Science, Numéro 4, 2023, Page(s) 101237, ISSN 2666-3864
Éditeur: Elsevier
DOI: 10.1016/j.xcrp.2022.101237

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

Auteurs: Duško Lainšček, Tina Fink, Vida Forstnerič, Iva Hafner-Bratkovič, Sara Orehek, Žiga Strmšek, Mateja Manček-Keber, Peter Pečan, Hana Esih, Špela Malenšek, Jana Aupič, Petra Dekleva, Tjaša Plaper, Sara Vidmar, Lucija Kadunc, Mojca Benčina, Neža Omersa, Gregor Anderluh, Florence Pojer, Kelvin Lau, David Hacker, Bruno E. Correia, David Peterhoff, Ralf Wagner, Valter Bergant, Alexander H
Publié dans: Vaccines, Numéro 9/5, 2021, Page(s) 431, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9050431

Influenza A (N1-N9) and Influenza B (B/Vic and B/Yam) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine design

Auteurs: Kelly A.S. da Costa, Joanne Marie M. Del Rosario, Matteo Ferrari, Sneha Vishwanath, Benedikt Asbach, Rebecca Kinsley, Ralf Wagner, Jonathan L. Heeney, George W. Carnell, Nigel J. Temperton
Publié dans: Vaccines , 2021, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.1101/2021.09.19.460966

Antigen-Triggered Logic-Gating of DNA Nanodevices

Auteurs: Wouter Engelen; Christian Sigl; Karoline Kadletz; Elena M. Willner; Hendrik Dietz
Publié dans: J Am Chem Soc, Numéro 1, 2021, Page(s) 21630-21636, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.1c09967

A Multiplexed Bacterial Two-Hybrid for Rapid Characterization of Protein-Protein Interactions and Iterative Protein Design

Auteurs: W. Clifford Boldridge, Ajasja Ljubetič, Hwangbeom Kim, Nathan Lubock, Dániel Szilágyi, Jonathan Lee, Andrej Brodnik, Roman Jerala, Sriram Kosuri
Publié dans: Nat Commun., 2024, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1101/2020.11.12.377184

Broad-Spectrum Virus Trapping with Heparan Sulfate-Modified DNA Origami Shells

Auteurs: Alba Monferrer, Jessica A. Kretzmann, Christian Sigl, Pia Sapelza, Anna Liedl, Barbara Wittmann, and Hendrik Dietz
Publié dans: ACS Nano, 2022, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.1c11328

Hepatitis B virus neutralization with DNA origami nanoshells

Auteurs: Elena M. Willner, Fenna Kolbe, Frank Momburg, Ulrike Protzer, Hendrik Dietz
Publié dans: ACS Appl Mater Interfaces., 2023, ISSN 1944-8244
Éditeur: American Chemical Society
DOI: 10.1101/2023.12.20.572526

Mucoadhesive film for oral delivery of vaccines for protection of the respiratory tract.

Auteurs: Esih H, Mezgec K, Billmeier M, Malenšek Š, Benčina M, Grilc B, Vidmar S, Gašperlin M, Bele M, Zidarn M, Zupanc TL, Morgan T, Jordan I, Sandig V, Schrödel S, Thirion C, Protzer U, Wagner R, Lainšček D, Jerala R.
Publié dans: Bioorg Chem, 2024, ISSN 0045-2068
Éditeur: Academic Press

Applications of HBVenv-specific antibodies as research and therapeutic tools

Auteurs: Kolbe, Fenna Meret
Publié dans: 2023
Éditeur: Technische Universität München

Programmable DNA origami shell platform for virus neutralization

Auteurs: Sigl, Christian
Publié dans: 2022
Éditeur: Fakultät für Physik

Functional DNA Origami Shells for Broad-Spectrum Antiviral Applications

Auteurs: Alba Monferrer i Sureda
Publié dans: 2023
Éditeur: TUM School of Natural Sciences

Nanoschalen aus DNA fangen und neutralisieren Viren

Auteurs: Christian Sigl, Hendrik Dietz
Publié dans: BIOspektrum, Numéro 28, 2022, Page(s) 165-167, ISSN 0947-0867
Éditeur: Spektrum Akademischer Verlag GmbH
DOI: 10.1007/s12268-022-1729-2

Droits de propriété intellectuelle

PROGRAMMABLE SHELLS FOR VIRUS ENCAPSULATION

Numéro de demande/publication: 20 158577
Date: 2020-02-20
Demandeur(s): TECHNISCHE UNIVERSITAET MUENCHEN

CONFORMATION SWITCHING NANOSTRUCTURES

Numéro de demande/publication: 21 169934
Date: 2021-04-22
Demandeur(s): TECHNISCHE UNIVERSITAET MUENCHEN

LIPOSOME-RECEPTOR-ASSAY FOR DETECTING NEUTRALIZING ANTIBODIES

Numéro de demande/publication: 20 217081
Date: 2020-12-23

CONFORMATION SWITCHING NANOSTRUCTURES

Numéro de demande/publication: 21 169934
Date: 2021-04-22

LIPOSOME-RECEPTOR-ASSAY FOR DETECTING NEUTRALIZING ANTIBODIES

Numéro de demande/publication: 20 217081
Date: 2020-12-23
Demandeur(s): UNIVERSITAET REGENSBURG

Livrables

Publications

Droits de propriété intellectuelle

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