General-purpose virus-neutralizing engulfing shells with modular target-specificity

Livrables

Public website: the website will present the partners, the overall research goals and objectives and regular updates about achievements (publications, patents), ongoing events, symposium and related activities from other entities around the world that are of interest.

VIROFIGHT communication kit

VIROFIGHT communication kit: This deliverable covers project flyer, slide show presentation (powerpoint, keynote) and poster.

Report on polymerized nucleic acids as engulfing shells on viruses

Report on polymerized nucleic acids as engulfing shells on virusesTriangular subunits for prefabricated shells furnished with virusspecific aptamers such that shell assembly will be templated by a virus surface AU will design small nucleic acid tiles featuring multiple aptamers that bind a repetitive subunit in the viral surface to yield 24 multivalent 46 nm shell building blocks with sticky DNA linkers stabilized into duplexes by the avidity effect from the viral surface CryoEM and electrophoretic mobility shift assays will be used to assess particle shape integrity and proper basic functionality Designs will be iteratively improved Report will cover results of these actions

Generation of non-infectious and infectious virus particles from non-enveloped viruses

Generation of noninfectious and infectious virus particles from nonenveloped virusesThis deliverable comprises solutions containing virus particles to be handed over to partners of the consortium for further experiments

Report on library of prototype DNA origami shell designs

Report on library of prototype DNA origami shell designs: This report will contain blueprints, 3D models, and DNA sequences needed to build the shells design variants. In addition, wherever applicable, experimental data obtained on the designs will be added and discussed, comprising e.g. cryo EM images, electrophoretic mobility shift analysis. etc

Generation of VLPs and LVs pseudotyped with surface proteins of enveloped viruses

Generation of VLPs and LVs pseudotyped with surface proteins of enveloped viruses: This deliverable comprises solutions containing the virus-like particles and lentiviral pseudotyped particles to be handed over to partners of the consortium for further experiments.

Report on assembly procedures for prefabricated DNA origami shells

Report on assembly procedures for prefabricated DNA origami shellsIn this report experimental procedures will be described leading to successful assembly of prefabricated DNA origami shells including thermal annealing conditions reaction mixture conditions purification steps and quality control procedures In addition troubleshooting measures and inluence of environmental conditions such as temperature or cation content on assembly will be described

Dissemination and communication plan

Dissemination and communication planA detailed communication and dissemination plan covering all communication and dissemination activities required during the project and adapted to the different target audiences and objectives of the communication or dissemination measures

Generation of hepatitis B viruses as exemplary enveloped virus

Generation of hepatitis B viruses as exemplary enveloped virusThis deliverable comprises solutions containing the hepatitis B viruses to be handed over to partners of the consortium for further experiments

Exploitation Innovation Plan

An initial exploitation innovation plan will be presented in M12 An updated version will be created in M36

Design details and protocols for modular swap out of virus recognizing moieties

Design details and protocols for modular swap out of virus recognizing moietiesSubsets of shellforming DNA oligonucleotides will be modified with terminal extensions pointing into the shell interior The sequences of the extensions will first be dummies to deal with and remove potential aggregation issues induced through Velcro effects Iterative refinements will be made so that arbitrary sequences can be attached in tunable decoration density and geometry in a plug and play fashion without affecting the shell quality and functionality AU will deliver virusbinding candidate DNA aptamer sequences to be included as direct terminal extensions or sticky linkers

Data Management Plan

Publications

Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection

Auteurs: Del Rosario JMM, da Costa KAS, Asbach B, Ferrara F, Ferrari M, Wells DA, Mann GS, Ameh VO, Sabeta CT, Banyard AC, Kinsley R, Scott SD, Wagner R, Heeney JL, Carnell GW, Temperton NJ.
Publié dans: Vaccines, 2021, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9070741

Influenza A (N1-N9) and Influenza B (B/Victoria and B/Yamagata) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine Design

Auteurs: da Costa KAS, Del Rosario JMM, Ferrari M, Vishwanath S, Asbach B, Kinsley R, Wagner R, Heeney JL, Carnell GW, Temperton NJ.
Publié dans: Vaccines, Numéro 2022 Sep 14;10(9), 2022, Page(s) 1520, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines10091520

Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses

Auteurs: Sampson AT, Heeney J, Cantoni D, Ferrari M, Sans MS, George C, Di Genova C, Mayora Neto M, Einhauser S, Asbach B, Wagner R, Baxendale H, Temperton N, Carnell G.
Publié dans: Viruses (Basel), 2021, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13081579

A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.

Auteurs: Valero J, Civit L, Dupont DM, Selnihhin D, Reinert LS, Idorn M, Israels BA, Bednarz AM, Bus C, Asbach B, Peterhoff D, Pedersen FS, Birkedal V, Wagner R, Paludan SR, Kjems J.
Publié dans: Proc Natl Acad Sci, Numéro 2021 Dec 14;118(50), 2021, ISSN 1091-6490
Éditeur: National Academy of Science
DOI: 10.1073/pnas.2112942118

Programmable icosahedral shell system for virus trapping

Auteurs: Christian Sigl, Elena M. Willner, Wouter Engelen, Jessica A. Kretzmann, Ken Sachenbacher, Anna Liedl, Fenna Kolbe, Florian Wilsch, S. Ali Aghvami, Ulrike Protzer, Michael F. Hagan, Seth Fraden, Hendrik Dietz
Publié dans: Nature Materials, 2021, ISSN 1476-1122
Éditeur: Nature Publishing Group
DOI: 10.1038/s41563-021-01020-4

Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein.

Auteurs: Staufer O, Gupta K, Hernandez Bücher JE, Kohler F, Sigl C, Singh G, Vasileiou K, Yagüe Relimpio A, Macher M, Fabritz S, Dietz H, Cavalcanti Adam EA, Schaffitzel C, Ruggieri A, Platzman I, Berger I, Spatz JP
Publié dans: Nat Commun, Numéro 2022 Feb 14;13(1), 2022, Page(s) 868, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-022-28446-x

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

Auteurs: Duško Lainšček, Tina Fink, Vida Forstnerič, Iva Hafner-Bratkovič, Sara Orehek, Žiga Strmšek, Mateja Manček-Keber, Peter Pečan, Hana Esih, Špela Malenšek, Jana Aupič, Petra Dekleva, Tjaša Plaper, Sara Vidmar, Lucija Kadunc, Mojca Benčina, Neža Omersa, Gregor Anderluh, Florence Pojer, Kelvin Lau, David Hacker, Bruno E. Correia, David Peterhoff, Ralf Wagner, Valter Bergant, Alexander H
Publié dans: Vaccines, Numéro 9/5, 2021, Page(s) 431, ISSN 2076-393X
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9050431

Antigen-Triggered Logic-Gating of DNA Nanodevices

Auteurs: Wouter Engelen; Christian Sigl; Karoline Kadletz; Elena M. Willner; Hendrik Dietz
Publié dans: J Am Chem Soc, Numéro 1, 2021, Page(s) 21630-21636, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.1c09967

Broad-Spectrum Virus Trapping with Heparan Sulfate-Modified DNA Origami Shells

Auteurs: Alba Monferrer, Jessica A. Kretzmann, Christian Sigl, Pia Sapelza, Anna Liedl, Barbara Wittmann, and Hendrik Dietz
Publié dans: ACS Nano, 2022, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.1c11328

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