MIXed plastics biodegradation and UPcycling using microbial communities

Resultado final

Related to task 82Final Data Management Plan

Related to task 8.2: Initial Data Management Plan

Online blog as platform for consistent public dissemination

Related to task 8.1: An online blog as platform for consistent public dissemination. After setting up the blog, frequent posts (English, minimum four per month) will report about the project’s progress and news from the global plastic crisis to set the consortium’s work in a comprehensive context. The blog will be linked to the social media channels of the consortium members to maximize the communication and impact (PGS).

Update SEVA collection

Related to task 7.7:Update SEVA collection with at least three new platforms for genome editing and site-specific sequence diversification of chromosomal segments (CSIC).

Publicaciones

Synthetic Biology and Genome-Editing Tools for Improving PHA Metabolic Engineering

Autores: Xu Zhang, Yina Lin, Qiong Wu, Ying Wang, Guo-Qiang Chen
Publicado en: Trends in Biotechnology, Edición 38/7, 2020, Página(s) 689-700, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2019.10.006

Biodegradation of polyester polyurethane by Cladosporium sp. P7: Evaluating its degradation capacity and metabolic pathways

Autores: Jiawei Liu, Qingyi Zeng, Huirui Lei, Kaiyuan Xin, Anming Xu, Ren Wei, Ding Li, Jie Zhou, Weiliang Dong, Min Jiang.
Publicado en: Journal of Hazardous Materials, 2023, ISSN 1873-3336
Editor: Elsevier
DOI: 10.1016/j.jhazmat.2023.130776

Enzymatic degradation of polyethylene terephthalate nanoplastics analyzed in real time by isothermal titration calorimetry

Autores: Kristina Vogel, Ren Wei, Lara Pfaff, Daniel Breite, Hassan Al-Fathi, Christian Ortmann, Irina Estrela-Lopis, Tom Venus, Agnes Schulze, Hauke Harms, Uwe T. Bornscheuer, Thomas Maskow
Publicado en: Science of The Total Environment, Edición 773, 2021, Página(s) 145111, ISSN 0048-9697
Editor: Elsevier BV
DOI: 10.1016/j.scitotenv.2021.145111

Anti-staphylococcal hydrogels based on bacterial cellulose and the antimicrobial biopolyester poly(3-hydroxy-acetylthioalkanoate-co-3-hydroxyalkanoate)

Autores: Virginia Rivero-Buceta, María Rosa Aguilar, Ana María Hernández-Arriaga, Francisco G. Blanco, Antonia Rojas, Marta Tortajada, Rosa Ana Ramírez-Jiménez, Blanca Vázquez-Lasa, Auxiliadora Prieto
Publicado en: International Journal of Biological Macromolecules, Edición 162, 2020, Página(s) 1869-1879, ISSN 0141-8130
Editor: Elsevier BV
DOI: 10.1016/j.ijbiomac.2020.07.289

Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform

Autores: Esteban Martínez-García, Víctor de Lorenzo
Publicado en: Current Opinion in Biotechnology, Edición 85, 2024, Página(s) 103025, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2023.103025

Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida

Autores: Pavel Dvořák, Teca Calcagno Galvão, Katharina Pflüger-Grau, Alice M Banks, Víctor de Lorenzo, Jose I Jiménez
Publicado en: Environmental Microbiology, 2023, ISSN 1462-2920
Editor: Wiley
DOI: 10.1111/1462-2920.16342

Chemical and biological catalysis for plastics recycling and upcycling

Autores: Lucas D. Ellis, Nicholas A. Rorrer, Kevin P. Sullivan, Maike Otto, John E. McGeehan, Yuriy Román-Leshkov, Nick Wierckx, Gregg T. Beckham
Publicado en: Nature Catalysis, Edición 4/7, 2021, Página(s) 539-556, ISSN 2520-1158
Editor: Springer Nature
DOI: 10.1038/s41929-021-00648-4

MIXed plastics biodegradation and UPcycling using microbial communities: EU Horizon 2020 project MIX-UP started January 2020

Autores: Hendrik Ballerstedt, Till Tiso, Nick Wierckx, Ren Wei, Luc Averous, Uwe Bornscheuer, Kevin O’Connor, Tilman Floehr, Andreas Jupke, Jürgen Klankermayer, Luo Liu, Victor de Lorenzo, Tanja Narancic, Juan Nogales, Rémi Perrin, Eric Pollet, Auxiliadora Prieto, William Casey, Thomas Haarmann, Alexandru Sarbu, Ulrich Schwaneberg, Fengxue Xin, Weiliang Dong, Jiamin Xing, Guo-Qiang Chen, Tianwei Tan, M
Publicado en: Environmental Sciences Europe, Edición 33/1, 2021, Página(s) 99, ISSN 2190-4707
Editor: Springer Verlag
DOI: 10.1186/s12302-021-00536-5

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

Autores: Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Publicado en: iScience, Edición 23/3, 2020, Página(s) 100946, ISSN 2589-0042
Editor: Elsevier
DOI: 10.1016/j.isci.2020.100946

Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization

Autores: Gerlis von Haugwitz, Xu Han, Lara Pfaff, Qian Li, Hongli Wei, Jian Gao, Karen Methling, Yufei Ao, Yannik Brack, Jan Mican, Christian G. Feiler, Manfred S. Weiss, David Bednar, Gottfried J. Palm, Michael Lalk, Michael Lammers, Jiri Damborsky, Gert Weber, Weidong Liu, Uwe T. Bornscheuer, Ren Wei
Publicado en: ACS Catalysis, 2022, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.2c03772

Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis

Autores: Lin-Ping Yu, Xu Yan, Xu Zhang, Xiang-Bin Chen, Qiong Wu, Xiao-Ran Jiang, Guo-Qiang Chen
Publicado en: Metabolic Engineering, Edición 59, 2020, Página(s) 119-130, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2020.02.005

Polyhydroxyalkanoates (PHA) toward cost competitiveness and functionality

Autores: Guo-Qiang Chen, Xin-Yu Chen, Fu-Qing Wu, Jin-Chun Chen
Publicado en: Advanced Industrial and Engineering Polymer Research, Edición 3/1, 2020, Página(s) 1-7, ISSN 2542-5048
Editor: Science Direct
DOI: 10.1016/j.aiepr.2019.11.001

A pseudo-homogeneous system for PET glycolysis using a colloidal catalyst of graphite carbon nitride in ethylene glycol

Autores: Zishuai Wang, Yaoqiang Wang, Shengnan Xu, Yu Jin, Zequn Tang, Gang Xiao, Haijia Su
Publicado en: Polymer Degradation and Stability, Edición 190, 2021, Página(s) 109638, ISSN 0141-3910
Editor: Elsevier BV
DOI: 10.1016/j.polymdegradstab.2021.109638

Molecular and Biochemical Differences of the Tandem and Cold-Adapted PET Hydrolases Ple628 and Ple629, Isolated From a Marine Microbial Consortium

Autores: Ingrid E. Meyer Cifuentes, Pan Wu, Yipei Zhao, Weidong Liu, Meina Neumann-Schaal, Lara Pfaff, Justyna Barys, Zhishuai Li, Jian Gao, Xu Han, Uwe T. Bornscheuer, Ren Wei, Basak Öztürk
Publicado en: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Editor: Frontiers Media S.A
DOI: 10.3389/fbioe.2022.930140

Production of PHA copolymers consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) by recombinant Halomonas bluephagenesis

Autores: Huan Wang, Jian-Wen Ye, Xinyu Chen, Yiping Yuan, Jingwen Shi, Xinyi Liu, Fang Yang, Yueyuan Ma, Jinchun Chen, Fuqing Wu, Yuxuan Lan, Qiong Wu, Yi Tong, Guo-Qiang Chen
Publicado en: Chemical Engineering Journal, Edición 466, 2024, Página(s) 143261, ISSN 1385-8947
Editor: Elsevier BV
DOI: 10.1016/j.cej.2023.143261

Enzymes for microplastic-free agricultural soils

Autores: Cristina Palacios-Mateo, Ke Meng, Lucia Legaz-Pol, Erik Steen Redeker, Esperanza Huerta-Lwanga, Lars M. Blank
Publicado en: Ecotoxicology and Environmental Safety, Edición 258, 2023, Página(s) 114982, ISSN 0147-6513
Editor: Academic Press
DOI: 10.1016/j.ecoenv.2023.114982

Engineering Halomonas bluephagenesis for L-Threonine production

Autores: Hetong Du, Yiqing Zhao, Fuqing Wu, Peifei Ouyang, Jinchun Chen, Xiaoran Jiang, Jianwen Ye, Guo-Qiang Chen
Publicado en: Metabolic Engineering, Edición 60, 2020, Página(s) 119-127, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2020.04.004

Hints from nature for a PHA circular economy: Carbon synthesis and sharing by Pseudomonas solani GK13

Autores: José D. Jiménez, Manuel S. Godoy, Carlos del Cerro, M. Auxiliadora Prieto
Publicado en: New Biotechnology, Edición 84, 2024, Página(s) 9-23, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2024.09.002

The molecular basis and enzyme engineering strategies for improvement of coupling efficiency in cytochrome P450s

Autores: Shuaiqi Meng, Yu Ji, LeiLei Zhu, Gaurao V. Dhoke, Mehdi D. Davari, Ulrich Schwaneberg
Publicado en: Biotechnology Advances, 2022, ISSN 0734-9750
Editor: Elsevier BV
DOI: 10.1016/j.biotechadv.2022.108051

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery.

Autores: Christian C. Blesken; Tessa Strümpfler; Till Tiso; Lars M. Blank
Publicado en: Microorganisms, Edición 2, 2020, ISSN 2078-2489
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.18154/rwth-2021-00396

Proposal for a systematic naming convention for liamocins

Autores: Till Tiso, Gina Welsing, Anna Lipphardt, Daniel F. Sauer, Zhenming Chi, Lars M. Blank, Heiko Hayen
Publicado en: Journal of Surfactants and Detergents, Edición 27, 2024, Página(s) 459-461, ISSN 1097-3958
Editor: American oil chemists' society Press
DOI: 10.1002/jsde.12735

For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!

Autores: Víctor de Lorenzo, Natalio Krasnogor, Markus Schmidt
Publicado en: New Biotechnology, Edición 60, 2021, Página(s) 44-51, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2020.08.004

Computational design of an efficient and thermostable esterase for polylactic acid depolymerization

Autores: Bin Xie, Jun Zhang, Huashan Sun, Rongrong Bai, Diannan Lu, Yushan Zhu, Weiliang Dong, Jie Zhou, Min Jiang
Publicado en: Green Chemistry, Edición 26, 2024, Página(s) 7268-7279, ISSN 1463-9262
Editor: Royal Society of Chemistry
DOI: 10.1039/d3gc04888h

Synthetic Control of Metabolic States in Pseudomonas putida by Tuning Polyhydroxyalkanoate Cycle

Autores: Maria-Tsampika Manoli, Juan Nogales, Auxiliadora Prieto
Publicado en: mBio, Edición January/February 2022 Volume 13 Edición 11, 2022, ISSN 2150-7511
Editor: American Society for Microbiology
DOI: 10.1128/mbio.01794-21

Sustainable cycloaliphatic polyurethanes from synthesis to applications

Autores: Agathe Mouren, Luc Avérous
Publicado en: Chem. Soc. Rev., Edición Vol. 52, 2023, Página(s) 277-317, ISSN 1460-4744
Editor: RSC
DOI: 10.1039/d2cs00509c

CRISPR/Cas9-enhanced targetron insertion for the delivery of heterologous sequences into the genome of Gram-negative bacteria

Autores: Elena Velázquez, Yamal Al-Ramahi, Víctor de Lorenzo
Publicado en: Current Protocols, Edición 2(9), 2022, Página(s) e532, ISSN 2691-1299
Editor: Wiley on line Library
DOI: 10.1002/cpz1.532

ArsH protects Pseudomonas putida from oxidative damage caused by exposure to arsenic

Autores: A. David Páez‐Espino, Pablo I. Nikel, Max Chavarría, Víctor Lorenzo
Publicado en: Environmental Microbiology, Edición 22/6, 2020, Página(s) 2230-2242, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14991

Pseudomonas umsongensis GO16 as a platform for the in vivo synthesis of short and medium chain length polyhydroxyalkanoate blends

Autores: Federico Cerrone, Binbin Zhou, Agathe Mouren, Luc Avérous, Stephen Conroy, Jeremy C. Simpson, Kevin E. O'Connor, Tanja Narancic
Publicado en: Bioresource Technology, Edición 387, 2024, Página(s) 129668, ISSN 0960-8524
Editor: Elsevier BV
DOI: 10.1016/j.biortech.2023.129668

Toward merging bottom–up and top–down model-based designing of synthetic microbial communities

Autores: David San León, Juan Nogales
Publicado en: Current Opinion in Microbiology, Edición Volume 69, October 2022, 102169, 2022, Página(s) 102169, ISSN 1369-5274
Editor: Elsevier BV
DOI: 10.1016/j.mib.2022.102169

Towards bio-upcycling of polyethylene terephthalate

Autores: Till Tiso, Tanja Narancic, Ren Wei, Eric Pollet, Niall Beagan, Katja Schröder, Annett Honak, Mengying Jiang, Shane T. Kenny, Nick Wierckx, Rémi Perrin, Luc Avérous, Wolfgang Zimmermann, Kevin O'Connor, Lars M. Blank
Publicado en: Metabolic Engineering, Edición 66, 2021, Página(s) 167-178, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2021.03.011

Advances in Aureobasidium research: Paving the path to industrial utilization

Autores: Difan Xiao, Marielle Driller, Marie Dielentheis‐Frenken, Frederick Haala, Philipp Kohl, Karla Stein, Lars M. Blank, Till Tiso
Publicado en: Microbial Biotechnology, Edición 17, 2024, ISSN 1751-7915
Editor: John Wiley & Sons Ltd.
DOI: 10.1111/1751-7915.14535

An updated structural model of the A domain of the Pseudomonas putida XylR regulator poses an atypical interplay with aromatic effectors

Autores: Pavel Dvořák, Carlos Alvarez‐Carreño, Sergio Ciordia, Alberto Paradela, Víctor Lorenzo
Publicado en: Environmental Microbiology, Edición 23/8, 2021, Página(s) 4418-4433, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15628

Tailor‐Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila

Autores: Mengyi Li, Yueyuan Ma, Xu Zhang, Lizhan Zhang, Xinyu Chen, Jian‐Wen Ye, Guo‐Qiang Chen
Publicado en: Advanced Materials, 2021, Página(s) 2102766, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202102766

Mixed plastics biodegradation and UPcycling using microbial communities: the NSFC-EU 2019 project MIX-UP to help achieve “carbon neutrality”

Autores: Jie Zhou, Haijia Su, Qiong Wu, Jianmin Xing, Weiliang Dong and Min Jiang
Publicado en: Chinese Journal of Biotechnology, 2021, ISSN 1000-3061
Editor: Kexue Chubaneshe/Science Press
DOI: 10.13345/j.cjb.210398

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Autores: Pablo I. Nikel, Tobias Fuhrer, Max Chavarría, Alberto Sánchez-Pascuala, Uwe Sauer, Víctor de Lorenzo
Publicado en: The ISME Journal, Edición 15/6, 2021, Página(s) 1751-1766, ISSN 1751-7362
Editor: Nature Publishing Group
DOI: 10.1038/s41396-020-00884-9

Novel Magnetic Carbon Supported Molybdenum Disulfide Catalyst and Its Application in Residue Upgrading

Autores: Xiangyang Zhu, Dong Qiao, Liangrong Yang, Qinling Bi, Huifang Xing, Shan Ni, Menglei Yuan, Huizhou Liu, Luhai Wang, An Ma
Publicado en: Green Energy & Environment, 2020, ISSN 2468-0257
Editor: ELSEVIER ScienceDirect
DOI: 10.1016/j.gee.2020.06.025

From Residues to Added-Value Bacterial Biopolymers as Nanomaterials for Biomedical Applications

Autores: Francisco G. Blanco, Natalia Hernández, Virginia Rivero-Buceta, Beatriz Maestro, Jesús M. Sanz, Aránzazu Mato, Ana M. Hernández-Arriaga, M. Auxiliadora Prieto
Publicado en: Nanomaterials, Edición 11/6, 2021, Página(s) 1492, ISSN 2079-4991
Editor: MDPI
DOI: 10.3390/nano11061492

Editorial: Microbial Degradation of Plastics

Autores: Ren Wei, Nick Wierckx
Publicado en: Frontiers in Microbiology, Edición 12, 2021, ISSN 1664-302X
Editor: Frontiers Media
DOI: 10.3389/fmicb.2021.635621

Synthesis of Modified Poly(vinyl Alcohol)s and Their Degradation Using an Enzymatic Cascade

Autores: Gerlis von Haugwitz, Kian Donnelly, Mara Di Filippo, Daniel Breite, Max Phippard, Agnes Schulze, Ren Wei, Marcus Baumann, Uwe Bornscheuer
Publicado en: Angew. Chem. Int. Ed., 2023, ISSN 1521-3773
Editor: Wiley
DOI: 10.1002/anie.202216962

Concomitant prediction of environmental fate and toxicity of chemical compounds

Autores: Juan Antonio Garcia-Martin, Max Chavarría, Victor de Lorenzo, Florencio Pazos
Publicado en: Biology Methods and Protocols, Edición 5/1, 2020, ISSN 2396-8923
Editor: Oxford University Press
DOI: 10.1093/biomethods/bpaa025

Process analysis of microplastic degradation using activated PMS and Fenton reagents

Autores: Bin Liu, Qixuan Jiang, Zenghui Qiu, Luo Liu, Ren Wei, Xin Zhang, Haijun Xu
Publicado en: Chemosphere, 2022, ISSN 0045-6535
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.chemosphere.2022.134220

Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida

Autores: Pavel Dvořák, Edward A. Bayer, Víctor de Lorenzo
Publicado en: ACS Synthetic Biology, Edición 9/10, 2020, Página(s) 2749-2764, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.0c00276

Enhanced bone regeneration via PHA scaffolds coated with polydopamine-captured BMP2

Autores: Xu Zhang, Jian Li, Jin Chen, Zi-Xin Peng, Jiang-Nan Chen, Xinyi Liu, Fuqing Wu, Peng Zhang, Guo-Qiang Chen
Publicado en: Journal of Materials Chemistry B, Edición 10, 2024, Página(s) 6214-6227, ISSN 2050-7518
Editor: Royal Society of Chemistry
DOI: 10.1039/d2tb01122k

Innovation versus novelty in microbial systems

Autores: de Lorenzo, Víctor
Publicado en: Environmental Microbioly, Edición 25(1), 2023, Página(s) 167-170, ISSN 1462-2920
Editor: Wiley
DOI: 10.1111/1462-2920.16278

ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces.

Autores: Yamal Al-ramahi, Akos Nyerges, Yago Margolles, Lidia Cerdán, Gyorgyi Ferenc, Csaba Pál, Luis Ángel Fernández, Víctor de Lorenzo
Publicado en: Communications Biology, Edición volume 4, Article number: 1169, 2021, ISSN 2399-3642
Editor: Nature Research
DOI: 10.1038/s42003-021-02702-0

The Cradle-to-Cradle Life Cycle Assessment of Polyethylene terephthalate: Environmental Perspective

Autores: Muhammad Tamoor, Nadia A. Samak, Maohua Yang, Jianmin Xing
Publicado en: Molecules, Edición 27, 2024, Página(s) 1599, ISSN 1420-3049
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27051599

Fabrication of hypercrosslinked hydroxyl-rich solid phase extractants for cesium separation from the salt lake brine

Autores: Zihao Xu, Meng Rong, Qiyu Meng, Haoyu Yao, Shan Ni, Li Wang, Huifang Xing, Hongnan Qu, Liangrong Yang, Huizhou Liu
Publicado en: Chemical Engineering Journal, Edición 400, 2020, Página(s) 125991, ISSN 1385-8947
Editor: Elsevier BV
DOI: 10.1016/j.cej.2020.125991

Possibilities and limitations of biotechnological plastic degradation and recycling

Autores: Ren Wei, Till Tiso, Jürgen Bertling, Kevin O’Connor, Lars M. Blank, Uwe T. Bornscheuer
Publicado en: Nature Catalysis, Edición Monthly journal, 2020, ISSN 2520-1158
Editor: Springer Nature
DOI: 10.1038/s41929-020-00521-w

A Standardized Inverter Package Borne by Broad Host Range Plasmids for Genetic Circuit Design in Gram-Negative Bacteria

Autores: Huseyin Tas, Ángel Goñi-Moreno, Víctor de Lorenzo
Publicado en: ACS Synthetic Biology, Edición 10/1, 2021, Página(s) 213-217, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.0c00529

A Combined Bio-Chemical Synthesis Route for 1-Octene Sheds Light on Rhamnolipid Structure

Autores: Till Tiso, Daniel F. Sauer, Klaus Beckerle, Christian C. Blesken, Jun Okuda, Lars M. Blank
Publicado en: Catalysts, Edición 10/8, 2020, Página(s) 874, ISSN 2073-4344
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal10080874

Efficient recovery of bio-based 1,2,4-butanetriol by using boronic acid anionic reactive extraction

Autores: Qiyu Meng, Jiemiao Yu, Liangrong Yang, Yanqing Li, Mo Xian, Huizhou Liu
Publicado en: Separation and Purification Technology, Edición 255, 2021, Página(s) 117728, ISSN 1383-5866
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2020.117728

Fusion of Chitin-Binding Domain From Chitinolyticbacter meiyuanensis SYBC-H1 to the Leaf-Branch Compost Cutinase for Enhanced PET Hydrolysis

Autores: Rui Xue, Yinping Chen, Huan Rong, Ren Wei, Zhongli Cui, Jie Zhou, Weiliang Dong, Min Jiang
Publicado en: Frontiers in Bioengineering and Biotechnology, 2021, ISSN 2296-4185
Editor: Frontiers Media S.A.
DOI: 10.3389/fbioe.2021.762854

Standardization of inducer-activated broad host range expression modules: Debugging and refactoring an alkane-responsive AlkS/PalkB device.

Autores: Alejandro Arce-Rodríguez, Ilaria Benedetti, José Manuel Borrero-de Acuña, Rafael Silva-Rocha, Víctor de Lorenzo
Publicado en: Synthetic Biology, Edición Volume 6, Edición 1, 2021, Página(s) 1–11, ISSN 2397-7000
Editor: Oxford University Press
DOI: 10.1093/synbio/ysab030

Enhanced resistance of Trichoderma harzianum LZDX-32-08 to hygromycin B induced by sea salt

Autores: Xijia Yu, Huiling Wei, Xianrui Liu, Dong Liu, Aili Fan, Haijia Su
Publicado en: Biotechnology Letters, Edición 43/1, 2021, Página(s) 213-222, ISSN 0141-5492
Editor: Kluwer Academic Publishers
DOI: 10.1007/s10529-020-02994-y

Hyphenation of supercritical fluid chromatography with different detection methods for identification and quantification of liamocin biosurfactants

Autores: Karen Scholz, Anna Lipphardt, Carina M. Wienken, Till Tiso, Heiko Hayen
Publicado en: Journal of Chromatography A, Edición 1631, 2020, Página(s) 461584, ISSN 0021-9673
Editor: Elsevier BV
DOI: 10.1016/j.chroma.2020.461584

Synthetic biology of extremophiles: a new wave of biomanufacturing

Autores: Jian-Wen Ye, Yi-Na Lin, Xue-Qing Yi, Zhuo-Xuan Yu, Xu Liu, Guo-Qiang Chen
Publicado en: Trends in Biotechnology, Edición 41, 2024, Página(s) 342-357, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2022.11.010

New insights into the biodegradation of polylactic acid: from degradation to upcycling

Autores: Bin Xu, Yinping Chen, Jie He, Shixiang Cao, Jiawei Liu, Rui Xue, Fengxue Xin, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Publicado en: Environmental Reviews, Edición 30, 2022, Página(s) 30-38, ISSN 1181-8700
Editor: NRC Research Press
DOI: 10.1139/er-2020-0117

Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes

Autores: Elena Velázquez, Beatriz Álvarez, Luis Ángel Fernández, Víctor de Lorenzo
Publicado en: Microbial Biotechnology, Edición 15(9), 2022, Página(s) 2309-2323, ISSN 1751-7915
Editor: Wiley Open Access
DOI: 10.1111/1751-7915.14098

Gaining control of bacterial cellulose colonization by polyhydroxyalkanoate-producing microorganisms to develop bioplasticized ultrathin films

Autores: Campano, Cristina; Rivero-Buceta, Virginia; Fabra, María José; Prieto, M Auxiliadora
Publicado en: International Journal of Biological Macromolecules, Edición Volume 223, Part A, 2022, Página(s) 1495-1505, ISSN 1879-0003
Editor: Elsevier B.V.
DOI: 10.1016/j.ijbiomac.2022.11.120

Quantitative assessment of morphological traits of planktonic bacterial aggregates

Autores: David R. Espeso, Esteban Martínez-García, Víctor de Lorenzo
Publicado en: Water Research, Edición 188, 2021, Página(s) 116468, ISSN 0043-1354
Editor: Elsevier BV
DOI: 10.1016/j.watres.2020.116468

Low CyaA expression and anti‐cooperative binding of cAMP to CRP frames the scope of the cognate regulon of Pseudomonas putida

Autores: Alejandro Arce‐Rodríguez, Pablo I. Nikel, Belén Calles, Max Chavarría, Raúl Platero, Tino Krell, Victor Lorenzo
Publicado en: Environmental Microbiology, Edición 23/3, 2021, Página(s) 1732-1749, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15422

Rapid colonization and biodegradation of untreated commercial polyethylene wrap by a new strain of Bacillus velezensis C5

Autores: Xianrui Liu, Yiming Zhang, Qiufeng Sun, Zihan Liu, Yilin Zhao, Aili Fan, Haijia Su
Publicado en: Journal of Environmental Management, 2021, ISSN 0301-4797
Editor: Academic Press
DOI: 10.1016/j.jenvman.2021.113848

Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Autores: Tanja Narancic, Manuel Salvador, Graham M. Hughes, Niall Beagan, Umar Abdulmutalib, Shane T. Kenny, Huihai Wu, Marta Saccomanno, Jounghyun Um, Kevin E. O'Connor, José I. Jiménez
Publicado en: Microbial Biotechnology, 2021, ISSN 1751-7915
Editor: WILEY
DOI: 10.1111/1751-7915.13712

Metabolic modelling approaches for describing and engineering microbial communities

Autores: Beatriz García-Jiménez, Jesús Torres-Bacete, Juan Nogales
Publicado en: Computational and Structural Biotechnology Journal, Edición 19, 2021, Página(s) 226-246, ISSN 2001-0370
Editor: Elsevier B.V.
DOI: 10.1016/j.csbj.2020.12.003

A SsrA/NIa-based Strategy for Post-Translational Regulation of Protein Levels in Gram-negative Bacteria

Autores: Gonzalo Durante-Rodríguez, Belén Calles, Víctor Lorenzo, Pablo Nikel
Publicado en: BIO-PROTOCOL, Edición 10/14, 2020, ISSN 2331-8325
Editor: Bio-101
DOI: 10.21769/bioprotoc.3688

The faulty SOS response of Pseudomonas putida KT2440 stems from an inefficient RecA‐LexA interplay

Autores: Özlem Akkaya, Tomás Aparicio, Danilo Pérez‐Pantoja, Víctor Lorenzo
Publicado en: Environmental Microbiology, Edición 23/3, 2021, Página(s) 1608-1619, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15384

Biodegradation of polyether-polyurethane foam in yellow mealworms (Tenebrio molitor) and effects on the gut microbiome

Autores: Jiawei Liu, Jingyuan Liu, Bin Xu, Anming Xu, Shixiang Cao, Ren Wei, Jie Zhou, Min Jiang, Weiliang Dong
Publicado en: Chemosphere, 2022, ISSN 0045-6535
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.chemosphere.2022.135263

Rational flux-tuning of Halomonas bluephagenesis for co-production of bioplastic PHB and ectoine

Autores: Hong Ma, Yiqing Zhao, Wuzhe Huang, Lizhan Zhang, Fuqing Wu, Jianwen Ye, Guo-Qiang Chen
Publicado en: Nature Communications, Edición 11/1, 2020, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-17223-3

Applications of phage-derived RNA-based technologies in synthetic biology

Autores: Wenhui Zhang, Qiong Wu
Publicado en: Synthetic and Systems Biotechnology, Edición 5/4, 2020, Página(s) 343-360, ISSN 2405-805X
Editor: Science Direct
DOI: 10.1016/j.synbio.2020.09.003

Can bioplastics always offer a truly sustainable alternative to fossil‐based plastics?

Autores: Lara Serrano‐Aguirre, M. Auxiliadora Prieto
Publicado en: Microbial Biotechnology, Edición 17, 2024, ISSN 1751-7915
Editor: John Wiley & Sons, Ltd
DOI: 10.1111/1751-7915.14458

The principle of uncertainty in biology: Will machine learning/artificial intelligence lead to the end of mechanistic studies?

Autores: Victor de Lorenzo
Publicado en: PLOS Biology, Edición 22, 2024, Página(s) e3002495, ISSN 1545-7885
Editor: Plos
DOI: 10.1371/journal.pbio.3002495

Naked Bacterium: Emerging Properties of a Surfome-Streamlined Pseudomonas putida Strain

Autores: Esteban Martínez-García, Sofía Fraile, David Rodríguez Espeso, Davide Vecchietti, Giovanni Bertoni, Víctor de Lorenzo
Publicado en: ACS Synthetic Biology, Edición 9/9, 2020, Página(s) 2477-2492, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.0c00272

The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future

Autores: Till Tiso, Benedikt Winter, RenWei, Johann Hee, Jan de Witt, NickWierckx, Peter Quicker, Uwe T. Bornscheuer, André Bardow, Juan Nogales, Lars M.Blank
Publicado en: Metabolic Engineering, 2021, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2021.12.006

Environmental Performance of Pseudomonas putida with a Uracylated Genome

Autores: Elena Algar, Yamal Al‐Ramahi, Víctor Lorenzo, Esteban Martínez‐García
Publicado en: ChemBioChem, Edición 21/22, 2020, Página(s) 3255-3265, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000330

Quantum Mechanical Investigation of the Oxidative Cleavage of the C–C Backbone Bonds in Polyethylene Model Molecules

Autores: Qixuan Jiang, Zhongyu Li, Ziheng Cui, Ren Wei, Kaili Nie, Haijun Xu, Luo Liu
Publicado en: Polymers, Edición 13/16, 2021, Página(s) 2730, ISSN 2073-4360
Editor: MDPI
DOI: 10.3390/polym13162730

Insight into formation and biological characteristics of Aspergillus tubingensis-based aerobic granular sludge (AT-AGS) in wastewater treatment

Autores: Yingyun Chen, Jiye Ge, Shaojie Wang, Haijia Su
Publicado en: Science of The Total Environment, Edición 739, 2020, Página(s) 140128, ISSN 0048-9697
Editor: Elsevier BV
DOI: 10.1016/j.scitotenv.2020.140128

Rapid degradation of poly(butylene succinate-co-butylene terephthalate)s by microbial communities at high-temperature

Autores: Hongyan Shen, Ye Yuan, Chuang Liu, Maohua Yang, Jianmin Xing
Publicado en: Biochemical Engineering Journal, Edición 204, 2024, Página(s) 109230, ISSN 1369-703X
Editor: Elsevier BV
DOI: 10.1016/j.bej.2024.109230

Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies

Autores: Sofía Fraile, María Briones, Mónica Revenga-Parra, Víctor de Lorenzo, Encarnación Lorenzo, Esteban Martínez-García
Publicado en: ACS Synthetic Biology, Edición 10/8, 2021, Página(s) 2049-2059, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.1c00227

Defined Microbial Mixed Culture for Utilization of Polyurethane Monomers

Autores: Romualdus Nugraha Catur Utomo, Wing-Jin Li, Till Tiso, Christian Eberlein, Moritz Doeker, Hermann J. Heipieper, Andreas Jupke, Nick Wierckx, Lars M. Blank
Publicado en: ACS Sustainable Chemistry & Engineering, Edición 8/47, 2020, Página(s) 17466-17474, ISSN 2168-0485
Editor: American Chemical Society
DOI: 10.1021/acssuschemeng.0c06019

Depolymerization of post-consumer PET bottles with engineered cutinase 1 from Thermobifida cellulosilytica

Autores: Zixuan Zhang, Shiming Huang, Di Cai, Chaofeng Shao, Changwei Zhang, Junhui Zhou, Ziheng Cui, Tianqi He, Changjing Chen, Biqiang Chen, Tianwei Tan
Publicado en: Green chemistry, Edición 14639270, 2022, ISSN 1463-9270
Editor: Royal Society of Chemistry
DOI: 10.1039/d2gc01834a

Application of a novel fluorogenic polyurethane analogue probe in polyester-degrading microorganisms screening by microfluidic droplet

Autores: Anming Xu, Jiawei Liu, Shixiang Cao, Bin Xu, Chengzhi Guo, Ziyi Yu, Xiaoqiang Chen, Jie Zhou, Weiliang Dong, Min Jiang
Publicado en: Microbial Biotechnology, 2022, ISSN 1751-7907
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1751-7915.14121

Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation

Autores: Jia, Yunpu Samak, Nadia A. Hao, Xuemi Chen, Zheng Yang, Gama Zhao, Xuhao Mu, Tingzhen Yang, Maohua Xing, Jianmin
Publicado en: Biochemical Engineering Journal, 2021, ISSN 1369-703X
Editor: Elsevier BV
DOI: 10.1016/j.bej.2021.108205

Contextual dependencies expand the re-usability of genetic inverters

Autores: Huseyin Tas; Lewis Grozinger; Ruud Stoof; V de Lorenzo; Angel Goñi-Moreno
Publicado en: Nature Communications, Edición 3, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1101/2020.07.15.204651

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2

Autores: Juhyun Kim, Angel Goñi-Moreno, Víctor de Lorenzo
Publicado en: mBio, Edición 12/1, 2021, ISSN 2161-2129
Editor: American Society for Microbiology
DOI: 10.1128/mbio.03685-20

Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers

Autores: Anming Xu, Xiaoxiao Zhang, Shixiang Cao, Xiaoli Zhou, Ziyi Yu, Xiujuan Qian, Jie Zhou, Weiliang Dong, and Min Jiang
Publicado en: ACS Synthetic Biology, 2022, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.1c00622

A Competitive High‐Throughput Screening Platform for Designing Polylactic Acid‐Specific Binding Peptides

Autores: Yi Lu, Kai‐Wolfgang Hintzen, Tetiana Kurkina, Yu Ji, Ulrich Schwaneberg
Publicado en: Advanced Science, Edición 10, 2024, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202303195

Construction of a Stable Expression System Based on the Endogenous hbpB/hbpC Toxin–Antitoxin System of Halomonas bluephagenesis

Autores: Kang Ren, Yiqing Zhao, Guo-Qiang Chen, Xiang Ao, Qiong Wu
Publicado en: ACS Synthetic Biology, Edición 13, 2024, Página(s) 61-67, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.3c00622

Synthetic Biology─High Time to Deliver?

Autores: Andrew D. Hanson, Víctor de Lorenzo
Publicado en: ACS Synthetic Biology, Edición 12, 2023, Página(s) 1579-1582, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.3c00238

Rapid depolymerization of poly(ethylene terephthalate) thin films by a dual-enzyme system and its impact on material properties

Autores: Natalia A. Tarazona, Ren Wei, Stefan Brott, Lara Pfaff, Uwe T. Bornscheuer, Andreas Lendlein, Rainhard Machatschek
Publicado en: Chem Catalysis, 2022, ISSN 2667-1093
Editor: Cell Press
DOI: 10.1016/j.checat.2022.11.004

When microbial biotechnology meets material engineering

Autores: Ana M. Hernandez-Arriaga, Cristina Campano, Virginia Rivero-Buceta and M. Auxiliadora Prieto
Publicado en: Microbial Biotechnology, Edición 2021, 2021, Página(s) 1-15, ISSN 1751-7915
Editor: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13975

Synthetic biology enabling a shift from domination to partnership with natural space

Autores: Víctor de Lorenzo, Miguel de la Ossa
Publicado en: Journal of Chinese Architecture and Urbanism, Edición 5, 2023, Página(s) 0619, ISSN 2717-5626
Editor: John Wiley
DOI: 10.36922/jcau.0619

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

Autores: Nora Lisa Bitzenhofer, Luzie Kruse, Stephan Thies, Benedikt Wynands, Thorsten Lechtenberg, Jakob Rönitz, Ekaterina Kozaeva, Nicolas Thilo Wirth, Christian Eberlein, Karl-Erich Jaeger, Pablo Iván Nikel, Hermann J. Heipieper, Nick Wierckx, Anita Loeschcke
Publicado en: Essays in Biochemistry, Edición 65, 2021, Página(s) 319-336, ISSN 0071-1365
Editor: Portland Press, Ltd.
DOI: 10.1042/ebc20200173

Standardization of regulatory nodes for engineering heterologous gene expression:a feasibility study

Autores: Pablo I. Nikel; Ilaria Benedetti; Nicolas T. Wirth; Víctor de Lorenzo; Belén Calles
Publicado en: Microbial Biotechnology, 2022, ISSN 1751-7915
Editor: Applied Microbiology and John Wiley & Sons Ltd.
DOI: 10.1111/1751-7915.14063

Mechanism-Based Design of Efficient PET Hydrolases

Autores: Ren Wei, Gerlis von Haugwitz, Lara Pfaff, Jan Mican, Christoffel P. S. Badenhorst, Weidong Liu, Gert Weber, Harry P. Austin, David Bednar, Jiri Damborsky, Uwe T. Bornscheuer
Publicado en: ACS Catalysis, 2022, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.1c05856

Unraveling 1,4-Butanediol Metabolism in Pseudomonas putida KT2440

Autores: Li, Wing Jin; Narancic, Tanja; Kenny, Shane T.; Niehoff, Paul-Joachim; O’Connor, Kevin; Blank, Lars M.; Wierckx, Nick
Publicado en: Frontiers in Microbiology, Edición 19, 2020, ISSN 1664-302X
Editor: Frontiers Media
DOI: 10.18154/rwth-2020-05721

Automated design and implementation of a NOR gate in Pseudomonas putida

Autores: Huseyin Tas, Lewis Grozinger, Angel Goñi-Moreno, Victor de Lorenzo
Publicado en: Synthetic Biology, Edición Volume 6, Edición 1, 2021, ISSN 2397-7000
Editor: Oxford University Press
DOI: 10.1093/synbio/ysab024

A self-stimulating system based on a polyhydroxyalkanoates coupled induction mechanism and its applications for Halomonas

Autores: Shuang Zheng, Zonghao Zhang, Peng Jiang, Jiale Wang, Yifei Zheng, Kang Ren, Lizhan Zhang, Xu Yan, Xu Liu, Guo-Qiang Chen
Publicado en: Chemical Engineering Journal, Edición 489, 2024, Página(s) 151413, ISSN 1385-8947
Editor: Elsevier BV
DOI: 10.1016/j.cej.2024.151413

Bio-upcycling of even and uneven medium-chain-length diols and dicarboxylates to polyhydroxyalkanoates using engineered Pseudomonas putida

Autores: Yannic S. Ackermann, Jan de Witt, Mariela P. Mezzina, Christoph Schroth, Tino Polen, Pablo I. Nikel, Benedikt Wynands and Nick Wierckx
Publicado en: Microbial Cell Factories, Edición 23, 2024, Página(s) 54, ISSN 1475-2859
Editor: BioMed Central
DOI: 10.1186/s12934-024-02310-7

Recent Advances in Bioplastics: Application and Biodegradation

Autores: Tanja Narancic, Federico Cerrone, Niall Beagan, Kevin E. O’Connor
Publicado en: Polymers, Edición 12/4, 2020, Página(s) 920, ISSN 2073-4360
Editor: MDPI
DOI: 10.3390/polym12040920

Biodegradation of low-density polyethylene by Microbulbifer hydrolyticus IRE-31

Autores: Zhongyu Li, Ren Wei, Meixi Gao, Yanru Ren, Bo Yu, Kaili Nie, Haijun Xu, Luo Liu
Publicado en: Journal of Environmental Management, Edición 263, 2020, Página(s) 110402, ISSN 0301-4797
Editor: Academic Press
DOI: 10.1016/j.jenvman.2020.110402

Thermophilic whole‐cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum

Autores: Fei Yan, Ren Wei, Qiu Cui, Uwe T. Bornscheuer, Ya‐Jun Liu
Publicado en: Microbial Biotechnology, 2020, ISSN 1751-7915
Editor: John Wiley & Sons Ltd
DOI: 10.1111/1751-7915.13580

Impact of the number of rhamnose moieties of rhamnolipids on the structure, lateral organization and morphology of model biomembranes

Autores: Marius Herzog; Lei Li; Christian C. Blesken; Gina Welsing; Till Tiso; Lars M. Blank; Roland Winter
Publicado en: Soft Matter, Edición 4, 2021, Página(s) 3191-3206, ISSN 1744-683X
Editor: Royal Society of Chemistry
DOI: 10.18154/rwth-2021-04514

Construction and comparison of synthetic microbial consortium system (SMCs) by non-living or living materials immobilization and application in acetochlor degradation

Autores: Jingyuan Liu, Xiaoli Zhou, Tong Wang, Lingling Fan, Shixun Liu, Nan Wu, Anming Xu, Xiujuan Qian, Zhoukun Li, Min Jiang, Jie Zhou, Weiliang Dong
Publicado en: Journal of Hazardous Materials, 2022, ISSN 0304-3894
Editor: Elsevier BV
DOI: 10.1016/j.jhazmat.2022.129460

Chemical recycling of bioplastics: technical opportunities to preserve chemical functionality as path towards a circular economy

Autores: Merchan AL, Fischöder T, Hee J, Lehnertz MS, Osterthun O, Pielsticker S, Schleier J, Tiso T, Blank LM, Klankermayer J, Kneer R, Quicker P, Walther G, Palkovits R
Publicado en: Green Chemistry, 2022, ISSN 1463-9262
Editor: Royal Society of Chemistry
DOI: 10.1039/d2gc02244c

Biocatalysis in the Recycling Landscape for Synthetic Polymers and Plastics towards Circular Textiles

Autores: Christina Jönsson, Ren Wei, Antonino Biundo, Johan Landberg, Lisa Schwarz Bour, Fabio Pezzotti, Andreea Toca, Les M. Jacques, Uwe T. Bornscheuer, Per‐Olof Syrén
Publicado en: ChemSusChem, 2021, ISSN 1864-5631
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.202002666

Foam control in biotechnological processes—challenges and opportunities

Autores: Till Tiso, Philipp Demling, Tobias Karmainski, Amira Oraby, Jens Eiken, Luo Liu, Patrick Bongartz, Matthias Wessling, Peter Desmond, Simone Schmitz, Sophie Weiser, Frank Emde, Hannah Czech, Juliane Merz, Susanne Zibek, Lars M. Blank, Lars Regestein
Publicado en: Discover Chemical Engineering, Edición 4, 2024, ISSN 2730-7700
Editor: Springer
DOI: 10.1007/s43938-023-00039-0

Flexibility Regulation of Loops Surrounding the Tunnel Entrance in Cytochrome P450 Enhanced Substrate Access Substantially

Autores: Zhongyu Li, Shuaiqi Meng, Kaili Nie, Ulrich Schwaneberg, Mehdi D. Davari, Haijun Xu, Yu Ji, and Luo Liu
Publicado en: ACS Catalysis, Edición 21555435, 2022, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.2c02258

Thermal and ultrasound foam control in biotechnology: a mini-review

Autores: Shahin Shah Khan; Irfan Ullah; Qingyun Wang; Haijun Xu; Till Tiso; Lars M. Blank; Luo Liu
Publicado en: Discover Chemical Engineering, 2022, ISSN 2730-7700
Editor: Springer
DOI: 10.1007/s43938-022-00016-z

Aromatic thermoplastic polyurethanes synthesized from different potential sustainable resources

Autores: Agathe Mouren, Luc Avérous
Publicado en: European Polymer Journal, Edición 197, 2023, Página(s) 112338, ISSN 0014-3057
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.eurpolymj.2023.112338

Effective Production of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Engineered Halomonas bluephagenesis Grown on Glucose and 1,4-Butanediol

Autores: Lizhan Zhang, Jian-Wen Ye,Xu Zhang, Wuzhe Huang, Zhongnan Zhang, Yina Lin, Ge Zhang, Fuqing Wu, Ziyu Wang, Qiong Wu, Guo-Qiang Chen
Publicado en: Bioresource Technology, Edición 2022-06, 2022, ISSN 1873-2976
Editor: Elsevier
DOI: 10.1016/j.biortech.2022.127270

Tunnel engineering for modulating the substrate preference in cytochrome P450BsβHI

Autores: Shuaiqi Meng, Ruipeng An, Zhongyu Li, Ulrich Schwaneberg, Yu Ji, Mehdi D. Davari, Fang Wang, Meng Wang, Meng Qin, Kaili Nie, Luo Liu
Publicado en: Bioresources and Bioprocessing, Edición 8, 2021, ISSN 2197-4365
Editor: Springer
DOI: 10.1186/s40643-021-00379-1

Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells

Autores: Thomas Bayer, Lara Pfaff, Yannick Branson, Aileen Becker, Shuke Wu, Uwe T.Bornscheuer, Ren Wei
Publicado en: iScience, 2022, ISSN 2589-0042
Editor: Elsevier Inc.
DOI: 10.1016/j.isci.2022.104326

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes

Autores: Esteban Martínez-García, Sofía Fraile, Elena Algar, Tomás Aparicio, Elena Velázquez, Belén Calles, Huseyin Tas, Blas Blázquez, Bruno Martín, Clara Prieto, Lucas Sánchez-Sampedro, Morten H H Nørholm, Daniel C Volke, Nicolas T Wirth, Pavel Dvořák, Lorea Alejaldre, Lewis Grozinger, Matthew Crowther, Angel Goñi-Moreno, Pablo I Nikel, Juan Nogales, Víctor de Lorenzo
Publicado en: Nucleic Acids Research, Edición 51(D1), 2023, Página(s) D1558-D1567, ISSN 1362-4962
Editor: Oxford University Press
DOI: 10.1093/nar/gkac1059

Flux optimization using multiple promoters in Halomonas bluephagenesis as a model chassis of the next generation industrial biotechnology

Autores: Yueyuan Ma, Jian-Wen Ye, Yina Lin, Xueqing Yi, Xuan Wang, Huan Wang, Ruiyan Huang, Fuqing Wu, Qiong Wu, Xu Liu, Guo-Qiang Chen
Publicado en: Metabolic Engineering, Edición 81, 2024, Página(s) 249-261, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2023.12.011

Recent advances in the improvement of bi-directional electron transfer between abiotic/biotic interfaces in electron-assisted biosynthesis system

Autores: Bin Xu, Zhe Li, Yujia Jiang, Minjiao Chen, Boryann Chen, Fengxue Xin, Weiliang Dong, Min Jiang
Publicado en: Biotechnology Advances, 2021, Página(s) 107810, ISSN 0734-9750
Editor: Elsevier BV
DOI: 10.1016/j.biotechadv.2021.107810

Engineering and evaluation of thermostable IsPETase variants for PET degradation

Autores: Stefan Brott, Lara Pfaff, Josephine Schuricht, Jan-Niklas Schwarz, Dominique Böttcher, Christoffel P. S. Badenhorst, Ren Wei, Uwe T. Bornscheuer
Publicado en: Engineering in Life Sciences, 2021, ISSN 1618-2863
Editor: John Wiley & Sons, Inc.
DOI: 10.1002/elsc.202100105

Biosynthesis of diverse α,ω-diol-derived polyhydroxyalkanoates by engineered Halomonas bluephagenesis

Autores: Xu Yan, Xu Liu, Lin-Ping Yu, Fuqing Wu, Xirao-Ran Jiang, Guo-Qiang Chen
Publicado en: Metabolic Engineering, Edición 2022-07, 2022, Página(s) 275-288, ISSN 1096-7184
Editor: Elsevier
DOI: 10.1016/j.ymben.2022.04.001

Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling

Autores: Nadia A. Samak, Yunpu Jia, Moustafa M. Sharshar, Tingzhen Mu, Maohua Yang, Sumit Peh, Jianmin Xing
Publicado en: Environment International, Edición 145, 2020, Página(s) 106144, ISSN 0160-4120
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2020.106144

Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects

Autores: Jiawei Liu, Jie He, Rui Xue, Bin Xu, Xiujuan Qian, Fengxue Xin, Lars M. Blank, Jie Zhou, Ren Wei, Weiliang Dong, Min Jiang
Publicado en: Biotechnology Advances, Edición 48, 2021, Página(s) 107730, ISSN 0734-9750
Editor: Elsevier BV
DOI: 10.1016/j.biotechadv.2021.107730

Current progress on the biodegradation of synthetic plastics: from fundamentals to biotechnological applications.

Autores: Andler R, Tiso T, Blank L, Andreeßen C, Zampolli J, D’Afonseca V, Guajardo C, Díaz-Barrera A
Publicado en: Reviews in Environmental Science and Bio/Technology, 2022, ISSN 1572-9826
Editor: Springer
DOI: 10.1007/s11157-022-09631-2

Engineering adipic acid metabolism in Pseudomonas putida

Autores: Yannic S. Ackermann, Wing-Jin Li, Leonie Op de Hipt, Paul-Joachim Niehoff, William Casey, Tino Polen, Sebastian Köbbing, Hendrik Ballerstedt, Benedikt Wynands, Kevin O'Connor, Lars M. Blank, Nick Wierckx
Publicado en: Metabolic Engineering, Edición 67, 2021, Página(s) 29-40, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2021.05.001

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Autores: Ángeles Hueso‐Gil, Belén Calles, Víctor Lorenzo
Publicado en: Environmental Microbiology, Edición 22/8, 2020, Página(s) 3535-3547, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15126

Future focuses of enzymatic plastic degradation

Autores: Anming Xu, Jie Zhou, Lars M. Blank, Min Jiang
Publicado en: Trends in Microbiology, Edición 31, 2023, Página(s) 668-671, ISSN 0966-842X
Editor: Elsevier BV
DOI: 10.1016/j.tim.2023.04.002

Feasible Cluster Model Method for Simulating the Redox Potentials of Laccase CueO and Its Variant

Autores: Qixuan Jiang, Ziheng Cui, Ren Wei, Kaili Nie, Haijun Xu, Luo Liu
Publicado en: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Editor: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.957694

Recombinant protein production in the filamentous fungus Trichoderma

Autores: Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su
Publicado en: Chinese Journal of Chemical Engineering, 2020, ISSN 1004-9541
Editor: Chemical Industry Press
DOI: 10.1016/j.cjche.2020.11.006

Comparative Analysis of the Behaviour of Marine Litter in Thermochemical Waste Treatment Processes

Autores: Johann Hee, Kai Schlögel, Simone Lechthaler, Jacqueline Plaster, Kristina Bitter, Lars Mathias Blank, Peter Quicker
Publicado en: Processes, Edición 9/1, 2021, Página(s) 13, ISSN 2227-9717
Editor: MDPI
DOI: 10.3390/pr9010013

An automated DIY framework for experimental evolution of Pseudomonas putida

Autores: David R. Espeso, Pavel Dvořák, Tomás Aparicio, Víctor Lorenzo
Publicado en: Microbial Biotechnology, 2020, Página(s) 1-7, ISSN 1751-7915
Editor: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13678

Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase

Autores: Lara Pfaff; Jian Gao; Zhishuai Li; Anna Jäckering; Gert Weber; Jan Mican; Yinping Chen; Weiliang Dong; Xu Han; Christian G. Feiler; Yu-Fei Ao; Christoffel P. S. Badenhorst; David Bednar; Gottfried J. Palm; Michael Lammers; Jiri Damborsky; Birgit Strodel; Weidong Liu; Uwe T. Bornscheuer; Ren Wei
Publicado en: ACS Catalysis, 2022, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.2c02275

Granulation enhancement and microbial community shift of tylosin-tolerant aerobic granular sludge on the treatment of tylosin wastewater

Autores: Wei Xiong, Shaojie Wang, Nan Zhou, Yingyun Chen, Haijia Su
Publicado en: Bioresource Technology, Edición 318, 2020, Página(s) 124041, ISSN 0960-8524
Editor: Elsevier BV
DOI: 10.1016/j.biortech.2020.124041

Picking the right metaphors for addressing microbial systems: economic theory helps understanding biological complexity

Autores: Juhyun Kim, Rafael Silva-Rocha, Víctor de Lorenzo
Publicado en: International Microbiology, 2021, ISSN 1139-6709
Editor: Sociedad Espanola de Microbiologia
DOI: 10.1007/s10123-021-00194-w

Grand Challenges for Industrializing Polyhydroxyalkanoates (PHAs)

Autores: Dan Tan, Ying Wang, Yi Tong, Guo-Qiang Chen
Publicado en: Trends in Biotechnology, Edición 39/9, 2021, Página(s) 953-963, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2020.11.010

Rapid Quantification of Polyhydroxyalkanoates Accumulated in Living Cells Based on Green Fluorescence Protein-Labeled Phasins: The qPHA Method

Autores: Xu Liu, Dianjie Li, Xu Yan, Zonghao Zhang, Shuang Zheng, Jingpeng Zhang, Wuzhe Huang, Fuqing Wu, Fangting Li, Guo-Qiang Chen
Publicado en: Biomacromolecules, Edición 2022-10-10, 2022, Página(s) 4153-4166, ISSN 1526-4602
Editor: American Chemical Society
DOI: 10.1021/acs.biomac.2c00624

Transcriptional control of 2,4‐dinitrotoluene degradation in Burkholderia sp . R34 bears a regulatory patch that eases pathway evolution

Autores: Danilo Pérez‐Pantoja, Pablo I. Nikel, Max Chavarría, Víctor Lorenzo
Publicado en: Environmental Microbiology, Edición 23/5, 2021, Página(s) 2522-2531, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15472

Recent Advances in Yeast Recombinant Biosynthesis of the Triterpenoid Protopanaxadiol and Glycosylated Derivatives Thereof

Autores: Shangkun Qiu, Lars M. Blank
Publicado en: Journal of Agricultural and Food Chemistry, Edición 71, 2023, Página(s) 2197-2210, ISSN 0021-8561
Editor: American Chemical Society
DOI: 10.1021/acs.jafc.2c06888

Applications of synthetic biology in medical and pharmaceutical fields

Autores: Xu Yan, Xu Liu, Cuihuan Zhao, Guo-Qiang Chen
Publicado en: Signal Transduction and Targeted Therapy, Edición 8, 2023, Página(s) 268-2670, ISSN 2059-3635
Editor: Nature Publishing Group
DOI: 10.1038/s41392-023-01440-5

Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes

Autores: Yi Lu, Kai-Wolfgang Hintzen, Tetiana Kurkina, Yu Ji, Ulrich Schwaneberg
Publicado en: ACS Catalysis, Edición 13, 2023, Página(s) 12746-12754, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.3c02142

Seawater activated TiO2 photocatalyst for degradation of organic compounds

Autores: Max-Fabian Volhard, Jonas Johannes Christ, Lars Mathias Blank, Thomas Jüstel
Publicado en: Sustainable Chemistry and Pharmacy, Edición 16, 2020, Página(s) 100251, ISSN 2352-5541
Editor: Elsevier
DOI: 10.1016/j.scp.2020.100251

The four Fs of the knowledge-based BioEconomy – A homage to Christian Patermann

Autores: Victor De Lorenzo
Publicado en: EFB Bioeconomy Journal, 2022, ISSN 2667-0410
Editor: Elsevier
DOI: 10.1016/j.bioeco.2022.100035

PHB production from food waste hydrolysates by Halomonas bluephagenesis Harboring PHB operon linked with an essential gene

Autores: Mengke Ji, Taoran Zheng, Ziyu Wang, Weijian Lai, Lizhan Zhang, Qianyi Zhang, Hongyi Yang, Si Meng, Wanghui Xu, Cuihuan Zhao, Qiong Wu, Guo-Qiang Chen
Publicado en: Metabolic Engineering, 2023, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2023.03.003

Hyperproduction of PHA copolymers containing high fractions of 4‐hydroxybutyrate (4HB) by outer membrane‐defected Halomonas bluephagenesis grown in bioreactors

Autores: Ziyu Wang; Yifei Zheng; Mengke Ji; Xu Zhang; Huan Wang; Yuemeng Chen; Qiong Wu; Guo‐Qiang Chen
Publicado en: Microbial Biotechnology, Edición 2022-05, 2022, Página(s) 1586-1597, ISSN 1751-7907
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1751-7915.13999

Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties

Autores: Ziyu Wang, Qin Qin, Yifei Zheng, Fajin Li, Yiqing Zhao, Guo-Qiang Chen
Publicado en: Metabolic Engineering, Edición 67, 2021, Página(s) 53-66, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2021.05.010

Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis

Autores: Xiao-Ran Jiang; Xiao-Ran Jiang; Xu Yan; Lin-Ping Yu; Xin-Yi Liu; Guo-Qiang Chen
Publicado en: Nature Communications, Edición 16, 2021, Página(s) 1531, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-21632-3

Golden Standard: A complete standard, portable, and interoperative MoClo tool for model and non-model bacterial hosts

Autores: Blas Blázquez, Jesús Torres-Bacete, David San Leon, Ryan Kniewel, Igor Martinez, Sandra Sordon, Aleksandra Wilczak, Sergio Salgado, Ewa Huszcza, Jarosław Popłoński, M Auxiliadora Prieto, Juan Nogales
Publicado en: bioRxiv, 2022, Página(s) 508659, ISSN 2692-8205
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.09.20.508659

Cyanamide as a Highly Efficient Organocatalyst for the Glycolysis Recycling of PET

Autores: Zishuai Wang, Yu Jin, Yaoqiang Wang, Zequn Tang, Shaojie Wang, Gang Xiao, Haijia Su
Publicado en: ACS Sustainable Chemistry & Engineering, 2023, Página(s) 7965−7973, ISSN 2168-0485
Editor: American Chemical Society
DOI: 10.1021/acssuschemeng.2c01235

High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy.

Autores: Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
Publicado en: MIMB,volume 2479, Edición 2479, 2022, Página(s) 37-52, ISBN 978-1-0716-2233-9
Editor: Humana, New York,
DOI: 10.1007/978-1-0716-2233-9_4

Großartige Winzlinge - Mikroben sind die heimlichen Herrscher der Welt. Und wir können sie für vieles nutzen.

Autores: Magdalena Hamm, Susan Schädlich, Anja Garms, Michael Stang
Publicado en: Das Magazin für neugierige Forscher, Edición "#1, April 2020", 2020, Página(s) 2-5
Editor: Bundesministerium für Bildung und Forschung (BMBF)

Waste to plastics by enzymes and bacteria - From plastic waste to PHA using enzymes and the bacterium P. putida

Autores: MT
Publicado en: bioplastics MAGAZINE, Edición 02/20 Vol. 15, 2020, Página(s) 22-24
Editor: Polymedia Publisher GmbH

Converting petrochemical plastic to biodegradable plastic

Autores: Narancic T., Wierckx N., Liu S., O'Connor K.E.
Publicado en: The Handbook of Polyhydroxyalkanoates – Microbial biosynthesis and feedstocks, 2020, ISBN 9780429296611
Editor: CRC press, Taylor & Francis Group

Microbes and Plastic – A Sustainable Duo for the Future

Autores: Birger Wolter, Henric M.T. Hintzen, Gina Welsing, Till Tiso, Lars M. Blank
Publicado en: Good Microbes in Medicine, Food Production, Biotechnology, Bioremediation, and Agriculture, Edición one time book publication, 2022, Página(s) 294-311, ISBN 9781119762621
Editor: John Wiley & Sons Ltd
DOI: 10.1002/9781119762621.ch24

Fluorimetric high-throughput screening method for polyester hydrolase activity using polyethylene terephthalate nanoparticles

Autores: Lara Pfaff, Daniel Breite, Christoffel P.S. Badenhorst, Uwe T. Bornscheuer, Ren Wei
Publicado en: Enzymatic Plastic Degradation, Edición 648, 2021, Página(s) 253-270, ISSN 0076-6879
Editor: Academic Press
DOI: 10.1016/bs.mie.2020.11.003

Anchor peptides promote degradation of mixed plastics for recycling

Autores: Yu Ji, Yi Lu, Hendrik Puetz, Ulrich Schwaneberg
Publicado en: Enzymatic Plastic Degradation, Edición 648, 2021, Página(s) 271-292, ISBN 9780128220122
Editor: Elsevier
DOI: 10.1016/bs.mie.2020.12.027

Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative

Autores: Gina Welsing, Birger Wolter, Henric M.T. Hintzen, Till Tiso, Lars M. Blank
Publicado en: Enzymatic Plastic Degradation, Edición 648, 2021, Página(s) 391-421, ISBN 9780128220122
Editor: Elsevier
DOI: 10.1016/bs.mie.2020.12.025

Biotechnological approach for polyurethane recycling: Separation of diamines from by-products via liquid-liquid extractio

Autores: Eberz, J., Doeker, M. and Jupke, A.
Publicado en: Chemie Ingenieur Technik, Edición 11, 2022, Página(s) 1351 - 1351, ISSN 1522-2640
Editor: Wiley-VCH GmbH
DOI: 10.1002/cite.202255341

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