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Aquaculture meets Biomedicine: Innovation in Skeletal Health research.

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

2 peer-reviewed papers/student (30).

Publications At least two technical articles are planned for publication in professional newspapers eg Fish Farming International andor magazines eg World Aquaculture or Aquaculture Europe summarizing the main results and outcomes of the project other than the scientific publications described in WP1

Individuation of indicators at different biological levels for early prediction of the onset and fate of skeletal anomalies in model species for marine (medaka) and freshwater (zebrafish) commercial fish

ESR4 Evaluation of skeletal anomalies requires a welldefined classification of the observed defects and an objective identification of the various types of defects

Primary effect of inappropriate P supply and function of Fgf23 on growth and development of bone tissue in Atlantic salmon.

ESR12 Phosphate P is a limiting resource directly related with organic pollution whereas unbalanced diets may affect animal health and welfare with occurrence of anomalies P content and efficiency in fish feeds based on plant ingredients is frequently low A phosphorus deficient vertebral phenotype in juvenile salmon will be used to test the effect of different factors on bone in an in vivo model

Technical articles

Overview articles presenting and analysing combined results of several ESRs

Evaluation of risk and prediction of skeleton anomalies.

ESR7 To precisely assess the burden of skeleton anomalies in European fish farms and to determine the socioeconomic impact regarding the prevention and treatment of skeleton anomalies in fish farms two main methodologies discrete choice analysis and risk assessment will be used

Biomedaqu Final workshop. All exploitation plans completed.

Final workshop and final reports All activities finished

Physiological mechanisms of Zn, Mn and Se effect on bone development and mineralization.

ESR6 Sustainable development of aquaculture asks for high quality ingredients to replace fishmeal and fish oil and fulfilling nutritional requirements This replacement presently leads to several skeletal alterations suggesting nutritional imbalances eg Zn Mn and Se are lower in plant ingredients Optimal dietary levels of Zn Mn and Se for skeletal health in seabream will be determined d14 M40 and the physiological mechanisms investigated through mutant fish models

Consortium Agreement

Consortium Agreement finalized

Biomedaqu Training schools

5 Summer Schools will be organized by different Beneficiaries under the leading management of ULG Each SS will be locally managed by the PI of the hosting Beneficiary The aim is to transfer skills in innovative tools to the recruited ESRs Partners will be actively involved in SS teaching As many as six Guidelines will be produced from SS

Recommendation of optimal levels and molecular forms of Zn, Mn and Se in commercial diets for gilthead seabream skeletal health.

ESR6 Sustainable development of aquaculture asks for high quality ingredients to replace fishmeal and fish oil and fulfilling nutritional requirements This replacement presently leads to several skeletal alterations suggesting nutritional imbalances eg Zn Mn and Se are lower in plant ingredients Optimal dietary levels of Zn Mn and Se for skeletal health in seabream will be determined

Report on the effect of vitamin K and D on bone metabolism of osteoblastic- and chondroblastic-like skeletal derived cells.

ESR8 The effects of VK and D supplementation on Ca metabolism bone development and mineralization and occurrence of skeletal anomalies will be investigated in gilthead seabream or meagre The involved physiological mechanisms will be studied through biochemical molecular cell culture and mutant fish studies

Satellite section in European Aquaculture Society Conference.

Organisation and participation of a satellite meeting at the Conference of the European Aquaculture Society

Predictions of the effect of preventive measures when applied by fish farmers.

Discrete choice experiments DCE have been used in many research fields to elicit preferences of decision makers regarding different products and services They involve the creation of hypothetical scenarios trying to mimic real markets which are defined to answer different research questions Thus it is possible to analyze the effect of important attributes over the outcomes of the experiment to quantify tradeoffs between attributes and to evaluate the willingness to pay for improving the quality of a product or service In our case different skeleton anomalies in form and severity will be defined and used Data will be obtained from a large number of companies and consumers within the consortium and through contacts from FEAP and API DCE provide rich data sources which have been widely used in health economics to evaluate preferences for healthcare products and services they allow researchers to obtain after the application of appropriate modelling methodologies such us discrete choice models merging their financial cost and revenues and their organizational consequences risks and controls to implement It will also provide essential inputs for the main economic evaluation techniques costbenefit analysis CBA and costutility analysis notably when coupled with riskcontrol matrix ULGCEPE

Dissemination and Outreach plan including website.

Disseminate project activities and results (d6.1, M4), both to professionals (fish farmers, stakeholders, researchers) and the public. Public reports from the different WPs will also be made available through the site. A page on a social network (i.e., Facebook) will be created and coordinated, directed for a larger non-scientific audience

Practical recommendations for fish farms and feed producers on vitamins K and D dietary levels to avoid bone anomalies and pathological effects of Ca metabolism alterations.

ESR8 Fatsoluble vitamins K VK and D VD play essential roles in Ca transport and tissue deposition and hence in bone development mineralization and remodelling Excessive VK and VD dietary levels can lead to bone anomalies there is a large variation in the content of these vitamins in live preys fed to marine fish larvae and their optimum levels during larval stages have not been yet determined for marine fish

In vivo and in vitro systems to test osteogenic activities

At the molecular level, skeletal formation and homeostasis depends on the presence of specific cells and their differentiation, proliferation and function in skeletal tissues influences extracellular matrix (ECM) and hydroxyapatite deposition. Osteoblast- and chondroblast-like skeletal derived cells from teleosts will be used as experimental model systems to assess the effects of micronutrients, at the cellular level, on the capacity to mineralize the ECM

BioMedAqC Supervisory Guidelines and ETN Training program.

Set up SB, Recruitment guidelines, Satellite Meetings and supervision infrastructure

Description of cellular basis of spine repair

ESR14 Scoliosis a deviation of the normal line of the spine is one the main ecological threats to commercial fish farming Hence understanding what leads to malformations of the axial skeleton in fish is not only of scientific interest but also has considerable economical relevance The complete understanding of the underlying biology has been hampered by an inability to clearly identify the cells which are responsible for mineralizing the axial skeleton and by an inability to follow the dynamics of axial skeletogenesis at the level of gene expression We have recently shown that the extracellular Entpd5 an enzyme providing phosphate for the mineralization process is expressed in a segmental manner within chordoblastsnotochord sheath cells of the notochord Lleras Forero submitted and have furthermore demonstrated that these cells have the propensity to repair damage inflicted to the notochord Lopez Baez submitted Based on these findings it will now be possible to understand in detail how expression of Entpd5 is orchestrated by the embryo to form the larval axial skeleton during physiological conditions and to repair skeletal elements upon pathophysiological damage

2 oral communications /student (30).

Publications At least two technical articles are planned for publication in professional newspapers eg Fish Farming International andor magazines eg World Aquaculture or Aquaculture Europe summarizing the main results and outcomes of the project other than the scientific publications described in WP1

Prediction model for early evaluation of skeletal quality in reared fish.

ESR4 Evaluation of skeletal anomalies requires a welldefined classification of the observed defects and an objective identification of the various types of defects d32 M40 Anatomical analysis of whole skeletons and data analysis will be embedded into the consolidated use of new generation analytic tools ie Kohonens SelfOrganizing Maps SOMs particularly suited for the analysis data that are nonlinear complex and characterized by internal redundancy or noise Together with ESR5 this will contribute to a better prediction and understanding of the phenomena leading to developmental osteological deformities

Report on the recruitment for all ESR posts, Consortium agreement.

Establishment of criteria for ESRs recruitment, announcement of ESR selection, selection and adoption in local Academic Doctorate Schools will be managed and coordinated as described in sec. 4.2. The lead partner is ULPGC, assisted in all steps by the SB

1 Conference proceedings (IAFSB).

Collection reviewing and publication of IAFSB Conference Proceedings

Crowding and space effects on skeleton system in advanced teleosts and recommendations issued for commercial farm.

ESR5 The use of large volumes and low densities have been demonstrated to be effective in lowering the incidence of skeletal anomalies in many marine reared species however the main cause driving these anomalies is unclear i smaller swimming space or ii the stress caused by too tight interactions among fish Identical batches of fish reared in two different volumes or at two different stocking densities ULPGCIPMAULG will be analyzed at the juvenile subadult and adult stage at anatomical UTV biomechanical INSERM histological UGent and biomolecular UALG WWU level in medaka and zebrafish and validated on commercial seabream

Cellular and molecular mechanisms of vertebral fusion in teleosts.

Fusion of vertebral bodies is a common pathology in farmed fish At the same time during evolution fusion of vertebral bodies became a requirement to establish the teleost caudal fin endoskeleton Vertebral body fusion can be both an important process in the frame of regular skeletal development and a pathological process Previous studies analyzed in depth the ontogenetically required vertebral body fusion that occurs during zebrafish caudal fin endoskeleton development Exact data sets exist about the timeline of vertebral body fusion the variability of the process and the microanatomy of the fusion process Fusion occurs on two levels a early on the level of mineralization of the notochord sheath that establishes vertebral body identity b late on the level of intramembranous perinotochordal bone formation ESR11 will use the welldocumented ontogenetically required fusion of the caudal most vertebral bodies to understand developmental and molecular mechanisms that underlay pathological vertebral body fusion in other parts of the teleost vertebral column

PhD awards.

All PhD awards granted

Tools to prevent pathological vertebral fusion in farmed fish.

Fusion of vertebral bodies is a common pathology in farmed fish At the same time during evolution fusion of vertebral bodies became a requirement to establish the teleost caudal fin endoskeleton Vertebral body fusion can be both an important process in the frame of regular skeletal development and a pathological process Previous studies analyzed in depth the ontogenetically required vertebral body fusion that occurs during zebrafish caudal fin endoskeleton development Exact data sets exist about the timeline of vertebral body fusion the variability of the process and the microanatomy of the fusion process Fusion occurs on two levels a early on the level of mineralization of the notochord sheath that establishes vertebral body identity b late on the level of intramembranous perinotochordal bone formation ESR11 will use the welldocumented ontogenetically required fusion of the caudal most vertebral bodies to understand developmental and molecular mechanisms that underlay pathological vertebral body fusion in other parts of the teleost vertebral column d39 M36 as a prerequisite to ensure healthy vertebral column development in fast growing fish under farming conditions

Function of new candidate genes in zebrafish bone formation. Relevance for human (and general) skeletal pathology.

ESR23 Zebrafish models have been proven relevant to study skeletal pathologies encountered in humans such as osteoporosis or scoliosis but possibly also in farmed fish ARS has identified the FIBULIN3 protein to be upregulated in serum of osteoarthritic patients UPD7 has identified several variants of genes in cohorts of young patients with osteoporosis osteoarthritis or ectopic mineral deposits in the cartilage such as chondrocalcinosis The function of these factors will be evaluated at the wholebody level by generating the zebrafish mutants in the genes of interest eg fbln1 fbln3 il11ra lrp5 wnt1 iftim5 plekhm1 using the CRISPR methodologies The mutant fish phenotype is characterized using established skeletal staining methods fluorescent transgenic lines microCT histomorphometric methods and by determining the expression of marker genes by in situ hybridization or RTPCR Variant proteins will be tested for proliferation in human and fish osteoblast cell culture Genes affecting skeletal development will be characterized in aquaculture species and their expression will be followed in challenging conditions that generate skeletal malformations including exposure to altered gravity conditions to evaluate their contribution to the defects

Biomedaqu workshops

As many as 1 international and 5 national workshops will be organized to disseminate results obtained in this Marie Curie Action among stakeholders Only ESRs recruited in the country will attend the local workshops while all recruited ESRs will attend the final International Workshop Lead partner is UALG all PIs of the Beneficiaries will be local organizers Local workshops aim to reach a stakeholder audience in which results obtained from this action will be disseminated The International Workshop is oriented toward both academic and enterprise audiences

Influence of space availability and stocking density on the skeletal system in medaka, zebrafish, and seabream at anatomic (skeletal deformities), histological (transdifferentiation and modeling) and molecular (Gla protein) levels.

ESR5 The use of large volumes and low densities have been demonstrated to be effective in lowering the incidence of skeletal anomalies in many marine reared species however the main cause driving these anomalies is unclear i smaller swimming space or ii the stress caused by too tight interactions among fish Identical batches of fish reared in two different volumes or at two different stocking densities ULPGCIPMAULG will be analyzed at the juvenile subadult and adult stage at anatomical UTV biomechanical INSERM histological UGent and biomolecular UALG WWU level in medaka and zebrafish and validated on commercial seabream

Supervisory Board

Supervisory board recruited.

Fish skeleton conference IAFSB 2021

Organisation and participation to the 6th IAFSB conference

Integrated web-based software for automatic classification of skeletal defects.

ESR1: Based on previous works and open-source tools to identify skeletal features in the head of 9-days old normal zebrafish larvae, we will develop an interactive tool to automatically identify, annotate and quantify skeletal elements in different types of images from fish. The tool will be first calibrated for analysis of normal and deficient zebrafish larvae, before extending to other types of skeletal images (fluorescent, X-ray), also of other species. Tools will be implemented for measuring sizes, relative positions and density of bone elements. Finally, a reporting tool including statistical analysis will be developed. Dissemination of these tools within the consortium and among the wider community will be implemented with the help of CTM

BioMedAqC Web page/intranet.

An BioMedAqC web page will be prepared to disseminate project activities and results

Publications

WNT11, a new gene associated with early-onset osteoporosis, is required for osteoblastogenesis.

Author(s): Caetano da Silva C, Edouard T, Fradin M, Aubert-Mucca M, Ricquebourg M, Raman R, Salles JP, Charon V, Guggenbuhl P, Muller M, Cohen-Solal M, Collet C
Published in: Human Molecular Genetics, Issue 31, 2021, Page(s) 1622-1634, ISSN 0964-6906
Publisher: Oxford University Press
DOI: 10.1093/hmg/ddab349

Zebrafish Tric-b is required for skeletal development and bone cells differentiation.

Author(s): Tonelli F, Leoni L, Daponte V, Gioia R, Cotti S, Fiedler IAK, Larionova D, Willaert A, Coucke PJ, Villani S, Busse B, Besio R, Rossi A, Witten PE, Forlino A
Published in: Front Endocrinol (Lausanne), Issue 14, 2023, Page(s) 1002914, ISSN 1664-2392
Publisher: Frontiers
DOI: 10.3389/fendo.2023.1002914

Zebrafish as a Model to Unveil the Pro-Osteogenic Effects of Boron-Vitamin D3 Synergism.

Author(s): Sojan JM, Gundappa MK, Carletti A, Gaspar V, Gavaia P, Maradonna F, Carnevali O
Published in: Front. Nutr., Issue 9, 2022, Page(s) 868805, ISSN 2296-861X
Publisher: Frontiers Media
DOI: 10.3389/fnut.2022.868805

Antioxidant and Anti-inflammatory Extracts From Sea Cucumbers and Tunicates Induce a Pro-osteogenic Effect in Zebrafish Larvae

Author(s): Alessio Carletti1,2†, Carlos Cardoso3,4†, Jorge Lobo-Arteaga5,6, Sabrina Sales3, Diana Juliao3, Inês Ferreira3, Paula Chainho6, Maria Ana Dionísio6, Maria J. Gaudêncio5, Cláudia Afonso3,4, Helena Lourenço3, M. Leonor Cancela1,2,7,8, Narcisa M. Bandarra3,4 and Paulo J. Gavaia1,2*
Published in: Front. Nutr., Issue 9, 2022, Page(s) 888360, ISSN 2296-861X
Publisher: Frontiers media S.A
DOI: 10.3389/fnut.2022.888360

A Baseline for Skeletal Investigations in Medaka (Oryzias latipes): The Effects of Rearing Density on the Postcranial Phenotype.

Author(s): Di Biagio C, Dellacqua Z, Martini A, Huysseune A, Scardi M, Witten PE, Boglione C
Published in: Frontiers in Endocrinology - Sec. Bone Research, Issue 560, 738505, 2022, ISSN 1664-2392
Publisher: Lausanne : Frontiers Research Foundation, 2010-
DOI: 10.3389/fendo.2022.893699

Vertebral column adaptations in juvenile Atlantic salmon Salmo salar, L. as a response to dietary phosphorus

Author(s): Lucia Drábiková, Per Gunnar Fjelldal, Adelbert De Clercq, M. Naveed Yousaf, Thea Morken, Charles McGurk, P. Eckhard Witten
Published in: Aquaculture, Issue 541, 2021, Page(s) 736776, ISSN 0044-8486
Publisher: Elsevier BV
DOI: 10.1016/j.aquaculture.2021.736776

Biopotential of Sea Cucumbers (Echinodermata) and Tunicates (Chordata) from the Western Coast of Portugal for the Prevention and Treatment of Chronic Illnesses

Author(s): Carletti A, Carlos Cardoso, Diana Juliao, Jorge L. Arteaga, Paula Chainho, Maria Ana Dionísio, Sabrina Sales, Maria J. Gaudêncio, Inês Ferreira, Cláudia Afonso, Helena Lourenço, M. Leonor Cancela, Narcisa M. Bandarra, Paulo J. Gavaia
Published in: Proceedings, Issue 61, 2020, Page(s) 13, ISSN 2504-3900
Publisher: MDPI
DOI: 10.3390/iecn2020-06994

Bacillus subtilis Modulated the Expression of Osteogenic Markers in a Human Osteoblast Cell Line

Author(s): Jerry Maria Sojan; Caterina Licini; Fabio Marcheggiani; Oliana Carnevali; Luca Tiano; Monica Mattioli-Belmonte; Francesca Maradonna
Published in: Cells, Issue 12, 2023, Page(s) 364, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells12030364

Understanding european aquaculture companies' perceived risks and risk management practices.

Author(s): Cantillo J, Van Caillie D
Published in: Aquaculture Economics & Management, 2023, ISSN 1365-7305
Publisher: International Association of Aquaculture Economics & Management
DOI: 10.1080/13657305.2022.2162625

Regular Supplementation with Antioxidants Rescues Doxorubicin-Induced Bone Deformities and Mineralization Delay in Zebrafish

Author(s): Sunil Poudel, Gil Martins, M. Leonor Cancela, Paulo J. Gavaia
Published in: Nutrients, Issue 14(23), 2022, Page(s) 4959, ISSN 2072-6643
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nu14234959

Probiotics Enhance Bone Growth and Rescue BMP Inhibition: New Transgenic Zebrafish Lines to Study Bone Health

Author(s): Sojan JM, Raman R, Muller M, Carnevali O, Renn J
Published in: International Journal of Molecular Sciences, Issue 23, 2022, Page(s) 4748, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23094748

Distinguishing the Effects of Water Volumes versus Stocking Densities on the Skeletal Quality during the Pre-Ongrowing Phase of Gilthead Seabream (Sparus aurata)

Author(s): Dellacqua Z, Di Biagio C, Costa C, Pousão-Ferreira P, Ribeiro L, Barata M, Gavaia P, Mattei F, Fabris A, Izquierdo M, Boglione C
Published in: Animals, Issue 13, 2023, Page(s) 557, ISSN 2076-2615
Publisher: MDPI
DOI: 10.3390/ani13040557

The essentials of marine biotechnology.

Author(s): Ana Rotter; Michéle Barbier; Francesco Bertoni; Francesco Bertoni; Atle M. Bones; M. Leonor Cancela; M. Leonor Cancela; Jens Carlsson; Maria F. Carvalho; Marta Cegłowska; Jerónimo Chirivella-Martorell; Meltem Conk Dalay; Mercedes Cueto; Thanos Dailianis; Irem Deniz; Ana R. Díaz-Marrero; Dragana Drakulovic; Arita Dubnika; Christine Edwards; Hjörleifur Einarsson; Ayşegül Erdoǧan; Orhan Tufan
Published in: Frontiers in marine science, Lausanne : Frontiers Media S.A., 2021, vol. 8, art. no. 629629, p. 1-53, Issue 20, 2021, ISSN 2296-7745
Publisher: Frontiers Media S.A
DOI: 10.3389/fmars.2021.629629

Understanding consumers' perceptions of aquaculture and its products in Gran Canaria island: Does the influence of positive or negative wording matter?

Author(s): Javier Cantillo; Concepción Román; Juan Carlos Martin
Published in: Aquaculture, Issue 15 January, 2023, Page(s) 738754, ISSN 0044-8486
Publisher: Elsevier BV
DOI: 10.1016/j.aquaculture.2022.738754

Resveratrol-Mediated Reversal of Doxorubicin-Induced Osteoclast Differentiation.Poudel S, Martins G, Cancela ML, Gavaia PJ.Int J Mol Sci. 2022 Dec 2;23(23):15160. doi: 10.3390/ijms232315160.

Author(s): Poudel S, Martins G, Cancela ML, Gavaia PJ.
Published in: Int J Mol Sci, Issue 23(23), 2022, Page(s) 15160, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232315160

Zebrafish caudal fin as a model to investigate the role of probiotics in bone regeneration.

Author(s): Sojan JM, Gioacchini G, Giorgini E, Orlando P, Tiano L, Maradonna F, Carnevali O
Published in: Scientific Reports, Issue 12, 2022, Page(s) 8057, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-12138-z

Visualization analysis of seabream and seabass aquaculture research using CiteSpace

Author(s): Javier Cantillo; Juan Carlos Martín; Concepción Román
Published in: Aquaculture Research, Issue 53, 2022, Page(s) 136-160, ISSN 1355-557X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/are.15560

Organic Selenium (OH-MetSe) Effect on Whole Body Fatty Acids and Mx Gene Expression against Viral Infection in Gilthead Seabream (Sparus aurata) Juveniles.

Author(s): Tseng Y, Dominguez D, Bravo J, Acosta F, Robaina L, Geraert PA, Kaushik S, Izquierdo M
Published in: Animals, Issue 11, 2021, Page(s) 2877, ISSN 2076-2615
Publisher: MDPI
DOI: 10.3390/ani11102877

What will happen to my smolt at harvest? Individually tagged Atlantic salmon help to understand possible progression and regression of vertebral deformities

Author(s): Lucia Drábiková; Per Gunnar Fjelldal; Adelbert De Clercq; M. Naveed Yousaf; Thea Morken; Charles McGurk; P. Eckhard Witten
Published in: Aquaculture, Issue 559, 2022, Page(s) 738430, ISSN 0044-8486
Publisher: Elsevier BV
DOI: 10.1016/j.aquaculture.2022.738430

Effects of the dietary supplementation of copper on growth, oxidative stress, fatty acid profile and skeletal development in gilthead seabream (Sparus aurata) larvae.

Author(s): Tseng. Y, Eryalçın. K.M, Sivagurunathan U, Domínguez D, Hernández-Cruz C.M, Boglione C, Antony Jesu Prabhu P, Izquierdo M.
Published in: Aquaculture, Issue 568, 2023, Page(s) 739319, ISSN 0044-8486
Publisher: Elsevier BV
DOI: 10.1016/j.aquaculture.2023.739319

wnt11f2 Zebrafish, an Animal Model for Development and New Insights in Bone Formation

Author(s): Caetano da Silva C, Ostertag A, Raman R, Muller M, Cohen-Solal M, Collet C
Published in: Zebrafish, Issue 20, 2023, Page(s) 1-9, ISSN 1545-8547
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/zeb.2022.0042

Determinants of fishery and aquaculture products consumption at home in the EU28

Author(s): Javier Cantillo, Juan Carlos Martín, Concepción Román
Published in: Food Quality and Preference, Issue 88, 2021, Page(s) 104085, ISSN 0950-3293
Publisher: Pergamon Press
DOI: 10.1016/j.foodqual.2020.104085

Discrete choice experiments in the analysis of consumers’ preferences for finfish products: A systematic literature review

Author(s): Javier Cantillo, Juan Carlos Martín, Concepción Román
Published in: Food Quality and Preference, Issue 84, 2020, Page(s) 103952, ISSN 0950-3293
Publisher: Pergamon Press
DOI: 10.1016/j.foodqual.2020.103952

Reversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development of Sparus aurata

Author(s): Sunil Poudel; Marisol Izquierdo; Maria Leonor Cancela; Paulo J. Gavaia
Published in: Nutrients; Volume 14; Issue 6; Pages: 1154, Issue 6, 2022, ISSN 2072-6643
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nu14061154

Crtap and p3h1 knock out zebrafish support defective collagen chaperoning as the cause of their osteogenesis imperfecta phenotype

Author(s): Tonelli F, Cotti S, Leoni L, Besio R, Gioia R, Marchese L, Giorgetti S, Villani S, Gistelinck C, Wagener R, Kobbe B, Fiedler IAK, Larionova D, Busse B, Eyre D, Rossi A, Witten PE, Forlino A
Published in: Matrix Biol, Issue 90, 2020, Page(s) 40-60, ISSN 0945-053X
Publisher: Elsevier BV
DOI: 10.1016/j.matbio.2020.03.004

More Bone with Less Minerals? The Effects of Dietary Phosphorus on the Post-Cranial Skeleton in Zebrafish

Author(s): Silvia Cotti, Ann Huysseune, Wolfgang Koppe, Martin Rücklin, Federica Marone, Eva M. Wölfel, Imke A. K. Fiedler, Björn Busse, Antonella Forlino, P. Eckhard Witten
Published in: International Journal of Molecular Sciences, Issue 21/15, 2020, Page(s) 5429, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21155429

Compression fractures and partial phenotype rescue with a low phosphorus diet in the chihuahua zebrafish osteogenesis imperfecta model

Author(s): Silvia Cotti; Ann Huysseune; Daria Larionova; Wolfgang Koppe; Antonella Forlino; Paul Eckhard Witten
Published in: Front Endocrinol, Issue 13, 2022, Page(s) 851879, ISSN 2352-1872
Publisher: Elsevier Inc.
DOI: 10.3389/fendo.2022.851879

A Best–Worst Measure of Attitudes toward Buying Seabream and Seabass Products: An Application to the Island of Gran Canaria

Author(s): Javier Cantillo, Juan Carlos Martín, Concepción Román
Published in: Foods, Issue 10/1, 2021, Page(s) 90, ISSN 2304-8158
Publisher: MDPI
DOI: 10.3390/foods10010090

A Hybrid Fuzzy TOPSIS Method to Analyze the Coverage of a Hypothetical EU Ecolabel for Fishery and Aquaculture Products (FAPs)

Author(s): Javier Cantillo, Juan Carlos Martín, Concepción Román
Published in: Applied Sciences, Issue 11/1, 2021, Page(s) 112, ISSN 2076-3417
Publisher: MDPI
DOI: 10.3390/app11010112

More severe phenotype of early‐onset osteoporosis associated with recessive form of LRP5 and combination with DKK1 or WNT3A

Author(s): Caroline Caetano da Silva, Manon Ricquebourg, Philippe Orcel, Stéphanie Fabre, Thomas Funck‐Brentano, Martine Cohen‐Solal, Corinne Collet
Published in: Molecular Genetics & Genomic Medicine, 2021, ISSN 2324-9269
Publisher: Wiley
DOI: 10.1002/mgg3.1681

Assessing the label’s mandatory information for fishery and aquaculture products in the EU28. A consumer approach based on a consistent fuzzy preference relation with geometric Bonferroni mean

Author(s): Javier Cantillo; Juan Carlos Martín; Concepción Román
Published in: Marine Policy, Issue 128, 2021, Page(s) 104515, ISSN 0044-8486
Publisher: Elsevier BV
DOI: 10.1016/j.marpol.2021.104515

Effects of dietary vitamin D3 levels on survival, mineralization, and skeletal development of gilthead seabream (Sparus aurata) larvae

Author(s): U. Sivagurunathan; David Dominguez; Yiyen Tseng; Kamil Mert Eryalçın; Javier Roo; Clara Boglione; P. Antony Jesu Prabhu; Marisol Izquierdo
Published in: Aquaculture, Issue 560, 2022, Page(s) 738505, ISSN 0044-8486
Publisher: Elsevier BV
DOI: 10.1016/j.aquaculture.2022.738505

A hybrid-fuzzy TOPSIS method to analyze the consumption and buying behavior of fishery and aquaculture products (FAPs) in the EU28

Author(s): Javier Cantillo, Juan Carlos Martin, Concepción Román
Published in: British Food Journal, Issue ahead-of-print/ahead-of-print, 2020, ISSN 0007-070X
Publisher: British Food Journal, Ltd
DOI: 10.1108/bfj-12-2019-0884

Analysis of the main determinants of away-from-home consumption of fishery and aquaculture products in the EU28

Author(s): Javier Cantillo; Juan Carlos Martín; Concepción Román
Published in: Appetite, Issue 163, 2021, Page(s) 105216, ISSN 0195-6663
Publisher: Elsevier BV
DOI: 10.1016/j.appet.2021.105216

Reversal of Doxorubicin-induced bone loss by antioxidant supplement

Author(s): Poudel, S, Izquierdo, M, M. Cancela, L, Gavaia, PJ
Published in: Bone reports, Issue 14, 2021, Page(s) 100984, ISSN 2352-1872
Publisher: Elsevier Inc.
DOI: 10.1016/j.bonr.2021.100984

Identification of bone anabolic compounds of marine origin using a zebrafish pipeline

Author(s): Rosa J, Carelli A, Varela T, Gavaia PJ, Cancela ML, Laizé V
Published in: Bone reports, Issue 14, 2021
Publisher: Elsevier
DOI: 10.1016/j.bonr.2021.100985

A reporter zebrafish line for live fluorescent visualisation of bone extracellular matrix

Author(s): Muller, Marc; Raman, Ratish; Guerrero Limon, Gustavo; Renn, Joerg
Published in: The International CRISPR and Gene Editing Symposium, Issue 1, 2020
Publisher: Mary Ann Liebert Inc.

More bone with less minerals : the effects of dietary phosphorus on the zebrafish skeleton

Author(s): Silvia Cotti; Ann Huysseune; Wolfgang Koppe; Martin Rücklin; Federica Marone; Eva M. Wölfel; Imke A.K. Fiedler; Björn Busse; Antonella Forlino; P. Eckhard Witten
Published in: BONE REPORTS, Issue 16, 2020, Page(s) 59-60, ISSN 2352-1872
Publisher: Elsevier Inc.
DOI: 10.1016/j.bonr.2022.101446

Deep Learning Approaches for Head and Operculum Segmentation in Zebrafish Microscopy Images

Author(s): Navdeep Kumar; Alessio Carletti; Paulo J. Gavaia; Marc Muller; M. Leonor Cancela; Pierre Geurts; Raphaël Marée
Published in: Computer Analysis of Images and Patterns ISBN: 9783030891275, Issue 18, 2021, ISBN 978-3-030-89128-2
Publisher: Springer
DOI: 10.1007/978-3-030-89128-2_15

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