Host switching pathogens, infectious outbreaks and zoonosis; a Marie Sklodowska-Curie Training Network

Resultado final

Website which includes information on the WPs, vacancies, results etc

Publicaciones

Mutagenic Analysis of Hazara Nairovirus Nontranslated Regions during Single- and Multistep Growth Identifies both Attenuating and Functionally Critical Sequences for Virus Replication

Autores: Daniele F. Mega, Jack Fuller, Beatriz Álvarez-Rodríguez, Jamel Mankouri, Roger Hewson, John N. Barr
Publicado en: Journal of Virology, Edición 94/17, 2020, ISSN 0022-538X
Editor: American Society for Microbiology
DOI: 10.1128/jvi.00357-20

Disparate temperature-dependent virus–host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium

Autores: Philip V’kovski, Mitra Gultom, Jenna N. Kelly, Silvio Steiner, Julie Russeil, Bastien Mangeat, Elisa Cora, Joern Pezoldt, Melle Holwerda, Annika Kratzel, Laura Laloli, Manon Wider, Jasmine Portmann, Thao Tran, Nadine Ebert, Hanspeter Stalder, Rune Hartmann, Vincent Gardeux, Daniel Alpern, Bart Deplancke, Volker Thiel, Ronald Dijkman
Publicado en: PLOS Biology, Edición 19/3, 2021, Página(s) e3001158, ISSN 1545-7885
Editor: PLOS
DOI: 10.1371/journal.pbio.3001158

West Nile Virus Epidemic in Germany Triggered by Epizootic Emergence, 2019

Autores: Ute Ziegler, Pauline Dianne Santos, Martin H. Groschup, Carolin Hattendorf, Martin Eiden, Dirk Höper, Philip Eisermann, Markus Keller, Friederike Michel, Robert Klopfleisch, Kerstin Müller, Doreen Werner, Helge Kampen, Martin Beer, Christina Frank, Raskit Lachmann, Birke Andrea Tews, Claudia Wylezich, Monika Rinder, Lars Lachmann, Thomas Grünewald, Claudia A. Szentiks, Michael Sieg, Jonas Schmi
Publicado en: Viruses, Edición 12/4, 2020, Página(s) 448, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12040448

Characterization and applications of a Crimean-Congo hemorrhagic fever virus nucleoprotein-specific Affimer: Inhibitory effects in viral replication and development of colorimetric diagnostic tests

Autores: Beatriz Álvarez-Rodríguez, Christian Tiede, Alexis C. R. Hoste, Rebecca A. Surtees, Chi H. Trinh, Gillian S. Slack, John Chamberlain, Roger Hewson, Alba Fresco, Patricia Sastre, Darren C. Tomlinson, Paul A. Millner, Thomas A. Edwards, John N. Barr
Publicado en: PLOS Neglected Tropical Diseases, Edición 14/6, 2020, Página(s) e0008364, ISSN 1935-2735
Editor: PLOS Neglected Tropical Diseases
DOI: 10.1371/journal.pntd.0008364

A CRISPR/Cas9 Generated Bovine CD46-knockout Cell Line—A Tool to Elucidate the Adaptability of Bovine Viral Diarrhea Viruses (BVDV)

Autores: Kevin P. Szillat, Susanne Koethe, Kerstin Wernike, Dirk Höper, Martin Beer
Publicado en: Viruses, Edición 12/8, 2020, Página(s) 859, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12080859

Pre-emptive genomic surveillance of emerging ebolaviruses

Autores: Ignacio Postigo-Hidalgo, Carlo Fischer, Andres Moreira-Soto, Patricia Tscheak, Michael Nagel, Markus Eickmann, Jan Felix Drexler
Publicado en: Eurosurveillance, Edición 25/3, 2020, ISSN 1560-7917
Editor: ECDC
DOI: 10.2807/1560-7917.es.2020.25.3.1900765

<p>Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland</p>

Autores: Joyce Odeke Akello, Richard Kamgang, Maria Teresa Barbani, Franziska Suter-Riniker, Stephen L Leib, Alban Ramette
Publicado en: Clinical Epidemiology, Edición Volume 12, 2020, Página(s) 353-366, ISSN 1179-1349
Editor: Dove Medical Press Ltd
DOI: 10.2147/clep.s246352

A Phylodynamic Workflow to Rapidly Gain Insights into the Dispersal History and Dynamics of SARS-CoV-2 Lineages

Autores: Simon Dellicour, Keith Durkin, Samuel L Hong, Bert Vanmechelen, Joan Martí-Carreras, Mandev S Gill, Cécile Meex, Sébastien Bontems, Emmanuel André, Marius Gilbert, Conor Walker, Nicola De Maio, Nuno R Faria, James Hadfield, Marie-Pierre Hayette, Vincent Bours, Tony Wawina-Bokalanga, Maria Artesi, Guy Baele, Piet Maes
Publicado en: Molecular Biology and Evolution, Edición 38/4, 2020, Página(s) 1608-1613, ISSN 1537-1719
Editor: Oxford University Press.
DOI: 10.1093/molbev/msaa284

Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test

Autores: Alexis C.R. Hoste, Angel Venteo, Alba Fresco-Taboada, Istar Tapia, Alejandro Monedero, Lissette López, Maarten F. Jebbink, Elisa Pérez-Ramírez, Miguel Angel Jimenez-Clavero, Mercedes Almonacid, Patricia Muñoz, Jesus Guinea, Carmen Vela, Lia van der Hoek, Paloma Rueda, Patricia Sastre
Publicado en: Diagnostic Microbiology and Infectious Disease, Edición 98/4, 2020, Página(s) 115167, ISSN 0732-8893
Editor: Elsevier BV
DOI: 10.1016/j.diagmicrobio.2020.115167

Seasonal coronavirus protective immunity is short-lasting

Autores: Arthur W. D. Edridge, Joanna Kaczorowska, Alexis C. R. Hoste, Margreet Bakker, Michelle Klein, Katherine Loens, Maarten F. Jebbink, Amy Matser, Cormac M. Kinsella, Paloma Rueda, Margareta Ieven, Herman Goossens, Maria Prins, Patricia Sastre, Martin Deijs, Lia van der Hoek
Publicado en: Nature Medicine, Edición 26/11, 2020, Página(s) 1691-1693, ISSN 1078-8956
Editor: Nature Publishing Group
DOI: 10.1038/s41591-020-1083-1

BKTyper: Free Online Tool for Polyoma BK Virus VP1 and NCCR Typing

Autores: Joan Martí-Carreras, Olga Mineeva-Sangwo, Dimitrios Topalis, Robert Snoeck, Graciela Andrei, Piet Maes
Publicado en: Viruses, Edición 12/8, 2020, Página(s) 837, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12080837

Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols

Autores: Annika Kratzel, Daniel Todt, Philip V’kovski, Silvio Steiner, Mitra Gultom, Tran Thi Nhu Thao, Nadine Ebert, Melle Holwerda, Jörg Steinmann, Daniela Niemeyer, Ronald Dijkman, Günter Kampf, Christian Drosten, Eike Steinmann, Volker Thiel, Stephanie Pfaender
Publicado en: Emerging Infectious Diseases, Edición 26/7, 2020, Página(s) 1592-1595, ISSN 1080-6040
Editor: US National Center for Infectious Diseases
DOI: 10.3201/eid2607.200915

At Least Seven Distinct Rotavirus Genotype Constellations in Bats with Evidence of Reassortment and Zoonotic Transmissions

Autores: Ceren Simsek, Victor Max Corman, Hermann Ulrich Everling, Alexander N. Lukashev, Andrea Rasche, Gael Darren Maganga, Tabea Binger, Daan Jansen, Leen Beller, Ward Deboutte, Florian Gloza-Rausch, Antje Seebens-Hoyer, Stoian Yordanov, Augustina Sylverken, Samuel Oppong, Yaw Adu Sarkodie, Peter Vallo, Eric M. Leroy, Mathieu Bourgarel, Kwe Claude Yinda, Marc Van Ranst, Christian Drosten, Jan Felix Drex
Publicado en: mBio, Edición 12/1, 2021, ISSN 2161-2129
Editor: American Society for Microbiology
DOI: 10.1128/mbio.02755-20

NCBI’s Virus Discovery Hackathon: Engaging Research Communities to Identify Cloud Infrastructure Requirements

Autores: Ryan Connor, Rodney Brister, Jan Buchmann, Ward Deboutte, Rob Edwards, Joan Martí-Carreras, Mike Tisza, Vadim Zalunin, Juan Andrade-Martínez, Adrian Cantu, Michael D’Amour, Alexandre Efremov, Lydia Fleischmann, Laura Forero-Junco, Sanzhima Garmaeva, Melissa Giluso, Cody Glickman, Margaret Henderson, Benjamin Kellman, David Kristensen, Carl Leubsdorf, Kyle Levi, Shane Levi, Suman Pakala, Vikas
Publicado en: Genes, Edición 10/9, 2019, Página(s) 714, ISSN 2073-4425
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10090714

NCBI’s Virus Discovery Codeathon: Building “FIVE” —The Federated Index of Viral Experiments API Index

Autores: Joan Martí-Carreras, Alejandro Rafael Gener, Sierra D. Miller, Anderson F. Brito, Christiam E. Camacho, Ryan Connor, Ward Deboutte, Cody Glickman, David M. Kristensen, Wynn K. Meyer, Sejal Modha, Alexis L. Norris, Surya Saha, Anna K. Belford, Evan Biederstedt, James Rodney Brister, Jan P. Buchmann, Nicholas P. Cooley, Robert A. Edwards, Kiran Javkar, Michael Muchow, Harihara Subrahmaniam Muralidh
Publicado en: Viruses, Edición 12/12, 2020, Página(s) 1424, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12121424

A Bacterially-Expressed Recombinant Envelope Protein from Usutu Virus Induces Neutralizing Antibodies in Rabbits

Autores: Kinga Böszörményi, Janet Hirsch, Gwendoline Kiemenyi Kayere, Zahra Fagrouch, Nicole Heijmans, Roberto Rodriguez Garcia, Soesjiel Dwarka, Amy van Dijke, Boyd Aaldijk, Ronald Limpens, Montserrat Barcena, Bram Koster, Babs Verstrepen, Willy Bogers, Clemens Kocken, Gesine Cornellissen, Ernst Verschoor, Bart Faber
Publicado en: Vaccines, Edición 9/2, 2021, Página(s) 157, ISSN 2076-393X
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9020157

Accounting for population structure reveals ambiguity in the Zaire Ebolavirus reservoir dynamics

Autores: Bram Vrancken, Tony Wawina-Bokalanga, Bert Vanmechelen, Joan Martí-Carreras, Miles W. Carroll, Justus Nsio, Jimmy Kapetshi, Sheila Makiala-Mandanda, Jean-Jacques Muyembe-Tamfum, Guy Baele, Kurt Vermeire, Valentijn Vergote, Steve Ahuka-Mundeke, Piet Maes
Publicado en: PLOS Neglected Tropical Diseases, Edición 14/3, 2020, Página(s) e0008117, ISSN 1935-2735
Editor: PLOS Neglected Tropical Diseases
DOI: 10.1371/journal.pntd.0008117

Evaluation of Viral RNA Recovery Methods in Vectors by Metagenomic Sequencing

Autores: Joyce Odeke Akello, Stephen L. Leib, Olivier Engler, Christian Beuret
Publicado en: Viruses, Edición 12/5, 2020, Página(s) 562, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12050562

Hazara Nairovirus Requires COPI Components in both Arf1-Dependent and Arf1-Independent Stages of Its Replication Cycle

Autores: J. Fuller, B. Álvarez-Rodríguez, E. J. A. A. Todd, J. Mankouri, R. Hewson, J. N. Barr
Publicado en: Journal of Virology, Edición 94/17, 2020, ISSN 0022-538X
Editor: American Society for Microbiology
DOI: 10.1128/jvi.00766-20

Susceptibility of well-differentiated airway epithelial cell cultures from domestic and wildlife animals to SARS-CoV-2

Autores: Mitra Gultom, Matthias Licheri, Laura Laloli, Manon Wider, Marina Strässle, Silvio Steiner, Annika Kratzel, Tran Thi Nhu Thao, Hanspeter Stalder, Jasmine Portmann, Melle Holwerda, Philip V’kovski, Nadine Ebert, Nadine Stokar – Regenscheit, Corinne Gurtner, Patrik Zanolari, Horst Posthaus, Simone Schuller, Amanda Vicente – Santos, Andres Moreira – Soto, Eugenia Corrales – Aguilar, Nicol
Publicado en: EID, 2021, ISSN 1080-6059
Editor: CDC
DOI: 10.3201/eid2707.204660

Preparedness needs research: How fundamental science and international collaboration accelerated the response to COVID-19

Autores: Cormac M. Kinsella, Pauline Dianne Santos, Ignacio Postigo-Hidalgo, Alba Folgueiras-González, Tim Casper Passchier, Kevin P. Szillat, Joyce Odeke Akello, Beatriz Álvarez-Rodríguez, Joan Martí-Carreras
Publicado en: PLOS Pathogens, Edición 16/10, 2020, Página(s) e1008902, ISSN 1553-7374
Editor: PLOS
DOI: 10.1371/journal.ppat.1008902

Co‐infections: Simultaneous detections of West Nile virus and Usutu virus in birds from Germany

Autores: Pauline Dianne Santos, Friederike Michel, Claudia Wylezich, Dirk Höper, Markus Keller, Cora M. Holicki, Claudia A. Szentiks, Martin Eiden, Aemero Muluneh, Antonie Neubauer‐Juric, Sabine Thalheim, Anja Globig, Martin Beer, Martin H. Groschup, Ute Ziegler
Publicado en: Transboundary and Emerging Diseases, 2021, ISSN 1865-1674
Editor: Blackwell-Wiss.-Verl
DOI: 10.1111/tbed.14050

Complete Genome Sequence of a New Ebola Virus Strain Isolated during the 2017 Likati Outbreak in the Democratic Republic of the Congo

Autores: Tony Wawina-Bokalanga, Bert Vanmechelen, Joan Martí-Carreras, Valentijn Vergote, Kurt Vermeire, Jean-Jacques Muyembe-Tamfum, Steve Ahuka-Mundeke, Piet Maes
Publicado en: Microbiology Resource Announcements, Edición 8/20, 2019, ISSN 2576-098X
Editor: Microbiology Resource Announcements
DOI: 10.1128/mra.00360-19

Rapid decline of Zika virus NS1 antigen-specific antibody responses, northeastern Brazil

Autores: Andres Moreira-Soto, Gilmara de Souza Sampaio, Célia Pedroso, Ignacio Postigo-Hidalgo, Beatrice Sarah Berneck, Sebastian Ulbert, Carlos Brites, Eduardo Martins Netto, Jan Felix Drexler
Publicado en: Virus Genes, Edición 56/5, 2020, Página(s) 632-637, ISSN 0920-8569
Editor: Kluwer Academic Publishers
DOI: 10.1007/s11262-020-01772-2

SARS-CoV-2 spike D614G change enhances replication and transmission

Autores: Bin Zhou, Tran Thi Nhu Thao, Donata Hoffmann, Adriano Taddeo, Nadine Ebert, Fabien Labroussaa, Anne Pohlmann, Jacqueline King, Silvio Steiner, Jenna N. Kelly, Jasmine Portmann, Nico Joel Halwe, Lorenz Ulrich, Bettina Salome Trüeb, Xiaoyu Fan, Bernd Hoffmann, Li Wang, Lisa Thomann, Xudong Lin, Hanspeter Stalder, Berta Pozzi, Simone de Brot, Nannan Jiang, Dan Cui, Jaber Hossain, Malania M. Wilson,
Publicado en: Nature, Edición 592/7852, 2021, Página(s) 122-127, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-021-03361-1

A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate

Autores: Lorena Sanchez-Felipe, Thomas Vercruysse, Sapna Sharma, Ji Ma, Viktor Lemmens, Dominique Van Looveren, Mahadesh Prasad Arkalagud Javarappa, Robbert Boudewijns, Bert Malengier-Devlies, Laurens Liesenborghs, Suzanne J. F. Kaptein, Carolien De Keyzer, Lindsey Bervoets, Sarah Debaveye, Madina Rasulova, Laura Seldeslachts, Li-Hsin Li, Sander Jansen, Michael Bright Yakass, Babs E. Verstrepen, Kinga P. B
Publicado en: Nature, Edición 590/7845, 2021, Página(s) 320-325, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-020-3035-9

STAT2 signaling restricts viral dissemination but drives severe pneumonia in SARS-CoV-2 infected hamsters

Autores: Robbert Boudewijns, Hendrik Jan Thibaut, Suzanne J. F. Kaptein, Rong Li, Valentijn Vergote, Laura Seldeslachts, Johan Van Weyenbergh, Carolien De Keyzer, Lindsey Bervoets, Sapna Sharma, Laurens Liesenborghs, Ji Ma, Sander Jansen, Dominique Van Looveren, Thomas Vercruysse, Xinyu Wang, Dirk Jochmans, Erik Martens, Kenny Roose, Dorien De Vlieger, Bert Schepens, Tina Van Buyten, Sofie Jacobs, Yanan Li
Publicado en: Nature Communications, Edición 11/1, 2020, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-19684-y

Host switching pathogens, infectious outbreaks and zoonosis: A Marie Skłodowska-Curie innovative training network (HONOURs)

Autores: Lia van der Hoek, Ernst Verschoor, Martin Beer, Dirk Höper, Kerstin Wernike, Marc Van Ranst, Jelle Matthijnssens, Piet Maes, Patricia Sastre, Paloma Rueda, Jan Felix Drexler, John Barr, Thomas Edwards, Paul Millner, Paul Vermeij, Ad de Groof, Volker Thiel, Ronald Dijkman, Franziska Suter-Riniker, Stephen Leib, Roger Koller, Alban Ramette, Olivier Engler, Christian Beuret
Publicado en: Virus Research, Edición 257, 2018, Página(s) 120-124, ISSN 0168-1702
Editor: Elsevier BV
DOI: 10.1016/j.virusres.2018.09.002

Synthetic viruses—Anything new?

Autores: Volker Thiel
Publicado en: PLOS Pathogens, Edición 14/10, 2018, Página(s) e1007019, ISSN 1553-7374
Editor: Public Library of Science
DOI: 10.1371/journal.ppat.1007019

Human cytomegalovirus genomics and transcriptomics through the lens of next-generation sequencing: revision and future challenges

Autores: Joan Martí-Carreras, Piet Maes
Publicado en: Virus Genes, Edición 55/2, 2019, Página(s) 138-164, ISSN 0920-8569
Editor: Kluwer Academic Publishers
DOI: 10.1007/s11262-018-1627-3

Novel Orthobunyavirus Identified in the Cerebrospinal Fluid of a Ugandan Child With Severe Encephalopathy

Autores: Arthur W D Edridge, Martin Deijs, Ruth Namazzi, Cosimo Cristella, Maarten F Jebbink, Irma Maurer, Neeltje A Kootstra, Linda R Buluma, Job B M van Woensel, Menno D de Jong, Richard Idro, Michael Boele van Hensbroek, Lia van der Hoek
Publicado en: Clinical Infectious Diseases, Edición 68/1, 2018, Página(s) 139-142, ISSN 1058-4838
Editor: University of Chicago Press
DOI: 10.1093/cid/ciy486

Enhanced bioinformatic profiling of VIDISCA libraries for virus detection and discovery

Autores: Cormac M. Kinsella, Martin Deijs, Lia van der Hoek
Publicado en: Virus Research, Edición 263, 2019, Página(s) 21-26, ISSN 0168-1702
Editor: Elsevier BV
DOI: 10.1016/j.virusres.2018.12.010

Viral Metagenomics on Cerebrospinal Fluid

Autores: Edridge, Deijs, van Zeggeren, Kinsella, Jebbink, Bakker, van de Beek, Brouwer, van der Hoek
Publicado en: Genes, Edición 10/5, 2019, Página(s) 332, ISSN 2073-4425
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10050332

Atypical Porcine Pestivirus Circulation and Molecular Evolution within an Affected Swine Herd

Autores: Alba Folgueiras-González, Robin van den Braak, Bartjan Simmelink, Martin Deijs, Lia van der Hoek, Ad de Groof
Publicado en: Viruses, Edición 12/10, 2020, Página(s) 1080, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v12101080

Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Autores: Alexis C. R. Hoste, Tamara Ruiz, Paloma Fernández‐Pacheco, Miguel Ángel Jiménez‐Clavero, Igor Djadjovski, Sandra Moreno, Alejandro Brun, Thomas A. Edwards, John N. Barr, Paloma Rueda, Patricia Sastre
Publicado en: Transboundary and Emerging Diseases, Edición 68/3, 2021, Página(s) 1229-1239, ISSN 1865-1674
Editor: Blackwell-Wiss.-Verl
DOI: 10.1111/tbed.13776

Full-genome sequencing of German rabbit haemorrhagic disease virus uncovers recombination between RHDV (GI.2) and EBHSV (GII.1)

Autores: Kevin P Szillat, Dirk Höper, Martin Beer, Patricia König
Publicado en: Virus Evolution, Edición 6/2, 2020, ISSN 2057-1577
Editor: Oxford University Press
DOI: 10.1093/ve/veaa080

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

Autores: Erik Alm, Eeva K Broberg, Thomas Connor, Emma B Hodcroft, Andrey B Komissarov, Sebastian Maurer-Stroh, Angeliki Melidou, Richard A Neher, Áine O’Toole, Dmitriy Pereyaslov
Publicado en: Eurosurveillance, Edición 25/32, 2020, ISSN 1560-7917
Editor: ECDC
DOI: 10.2807/1560-7917.es.2020.25.32.2001410

Entamoeba and Giardia parasites implicated as hosts of CRESS viruses

Autores: Cormac M. Kinsella, Aldert Bart, Martin Deijs, Patricia Broekhuizen, Joanna Kaczorowska, Maarten F. Jebbink, Tom van Gool, Matthew Cotten, Lia van der Hoek
Publicado en: Nature Communications, Edición 11/1, 2020, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-18474-w

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

Autores: Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Philip V’kovski, Hanspeter Stalder, Jasmine Portmann, Jenna Kelly, Silvio Steiner, Melle Holwerda, Annika Kratzel, Mitra Gultom, Kimberly Schmied, Laura Laloli, Linda Hüsser, Manon Wider, Stephanie Pfaender, Dagny Hirt, Valentina Cippà, Silvia Crespo-Pomar, Simon Schröder, Doreen Muth, Daniela Niemeyer, Victor M. Corman, Marcel A. Müller, C
Publicado en: Nature, Edición 582/7813, 2020, Página(s) 561-565, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-020-2294-9

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