Descrizione del progetto
Progettazione razionale di nuovi ceppi di lievito che producono composti lipidici e aromatici
La biotecnologia sfrutta i processi biologici per scopi industriali e di altro tipo. I lieviti sono simili a piccole fabbriche che vengono ampiamente utilizzate per i loro metaboliti, enzimi e proteine. Il lievito Saccharomyces cerevisiae è forse il più antico e il più utilizzato. Il progetto CHASSY, finanziato dall’UE, prevede di liberare tutto il potenziale del lievito ridisegnando i circuiti metabolici e ampliando la gamma di ospiti per includere il lievito oleaginoso Yarrowia lipolytica (in grado di accumulare quantità significative di lipidi o trigliceridi) e il lievito termotollerante Kluyveromyces marxianus. Il team utilizzerà gli strumenti della biologia sintetica per costruire nuovi ceppi per le fabbriche di cellule che producono tre prodotti di alto valore: gli oleochimici docosanolo e acido ottanoico e la molecola aromatica amorfrutina 1.
Obiettivo
CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.
Campo scientifico
Not validated
Not validated
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- natural scienceschemical sciencesorganic chemistryaromatic compounds
- natural scienceschemical sciencesorganic chemistryamines
- natural sciencesbiological sciencesgeneticsgenomes
Parole chiave
Programma(i)
Invito a presentare proposte
Vedi altri progetti per questo bandoBando secondario
H2020-NMBP-BIO-2016
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
T12 YN60 Cork
Irlanda