Bacterial evolution of hypersensitivity and resistance against antimicrobial peptides

Project Information

resistance evolution

Grant agreement ID: 648364

DOI 10.3030/648364

Project closed

EC signature date 28 September 2015

Start date 1 October 2015

End date 31 March 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 846 250,00

EU contribution € 1 846 250,00

Coordinated by

HUN-REN SZEGEDI BIOLOGIAI KUTATOKOZPONT
Hungary

Publications

Peer reviewed articles (28)

Recombineering and MAGE

Author(s): Timothy M. Wannier, Peter N. Ciaccia, Andrew D. Ellington, Gabriel T. Filsinger, Farren J. Isaacs, Kamyab Javanmardi, Michaela A. Jones, Aditya M. Kunjapur, Akos Nyerges, Csaba Pal, Max G. Schubert & George M. Church
Published in: Nature Reviews Methods Primers, 2021, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s43586-020-00006-x

Hybrid Inhibitors of DNA Gyrase A and B: Design, Synthesis and Evaluation.

Author(s): Martina Durcik; Žiga Skok; Janez Ilaš; Nace Zidar; Anamarija Zega; Petra Szili; Gábor Draskovits; Tamás Révész; Danijel Kikelj; Ákos Nyerges; Ákos Nyerges; Csaba Pál; Lucija Peterlin Mašič; Tihomir Tomašič
Published in: Pharmaceutics, volumne 13, chapter 6, Issue 2, 2020, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13010006

CRISPR-interference-based modulation of mobile genetic elements in bacteria

Author(s): Ákos Nyerges, Balázs Bálint, Judit Cseklye, István Nagy, Csaba Pál, Tamás Fehér
Published in: Synthetic Biology, Issue 4/1, 2019, ISSN 2397-7000
Publisher: Oxford Academic
DOI: 10.1093/synbio/ysz008

Rationally designed foldameric adjuvants enhance antibiotic efficacy via promoting membrane hyperpolarization

Author(s): Kaushik Nath Bhaumik; Anasztázia Hetényi; Gábor Olajos; Ana Martins; Réka Spohn; Lukács Németh; Balázs Jójárt; Petra Szili; Petra Szili; Anett Dunai; Pramod Kumar Jangir; Lejla Daruka; Lejla Daruka; Imre Földesi; D Kata; Csaba Pál; Tamás A. Martinek
Published in: Molecular Systems Design & Engineering, Issue 2, 2021, ISSN 2058-9689
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1me00118c

Dual Action of the PN159/KLAL/MAP Peptide: Increase of Drug Penetration across Caco-2 Intestinal Barrier Model by Modulation of Tight Junctions and Plasma Membrane Permeability

Author(s): Alexandra Bocsik, Ilona Gróf, Lóránd Kiss, Ferenc Ötvös, Ottó Zsíros, Lejla Daruka, Lívia Fülöp, Monika Vastag, Ágnes Kittel, Norbert Imre, Tamás Martinek, Csaba Pál, Piroska Szabó-Révész, Mária A. Deli
Published in: Pharmaceutics, Issue 11/2, 2019, Page(s) 73, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics11020073

Rational design of balanced dual-targeting antibiotics with limited resistance

Author(s): Akos Nyerges, Tihomir Tomašič, Martina Durcik, Tamas Revesz, Petra Szili, Gabor Draskovits, Ferenc Bogar, Žiga Skok, Nace Zidar, Janez Ilaš, Anamarija Zega, Danijel Kikelj, Lejla Daruka, Balint Kintses, Balint Vasarhelyi, Imre Foldesi, Diána Kata, Martin Welin, Raymond Kimbung, Dorota Focht, Lucija Peterlin Mašič, Csaba Pal
Published in: PLOS Biology, Issue 18/10, 2020, Page(s) e3000819, ISSN 1545-7885
Publisher: Public Library of Science
DOI: 10.1371/journal.pbio.3000819

Proteome-wide landscape of solubility limits in a bacterial cell

Author(s): Ádám Györkei, Lejla Daruka, Dávid Balogh, Erika Őszi, Zoltán Magyar, Balázs Szappanos, Gergely Fekete, Mónika Fuxreiter, Péter Horváth, Csaba Pál, Bálint Kintses & Balázs Papp
Published in: Scientific Reports, 2022, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-10427-1

Phylogenetic barriers to horizontal transfer of antimicrobial peptide resistance genes in the human gut microbiota

Author(s): Bálint Kintses, Orsolya Méhi, Eszter Ari, Mónika Számel, Ádám Györkei, Pramod K. Jangir, István Nagy, Ferenc Pál, Gergely Fekete, Roland Tengölics, Ákos Nyerges, István Likó, Anita Bálint, Tamás Molnár, Balázs Bálint, Bálint Márk Vásárhelyi, Misshelle Bustamante, Balázs Papp, Csaba Pál
Published in: Nature Microbiology, Issue 4/3, 2019, Page(s) 447-458, ISSN 2058-5276
Publisher: Nature Publishing Group
DOI: 10.1038/s41564-018-0313-5

Integrated evolutionary analysis reveals antimicrobial peptides with limited resistance

Author(s): Réka Spohn, Lejla Daruka, Viktória Lázár, Ana Martins, Fanni Vidovics, Gábor Grézal, Orsolya Méhi, Bálint Kintses, Mónika Számel, Pramod K. Jangir, Bálint Csörgő, Ádám Györkei, Zoltán Bódi, Anikó Faragó, László Bodai, Imre Földesi, Diána Kata, Gergely Maróti, Bernadett Pap, Roland Wirth, Balázs Papp, Csaba Pál
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-12364-6

Limited Evolutionary Conservation of the Phenotypic Effects of Antibiotic Resistance Mutations

Author(s): Gábor Apjok, Gábor Boross, Ákos Nyerges, Gergely Fekete, Viktória Lázár, Balázs Papp, Csaba Pál, Bálint Csörgő
Published in: Molecular Biology and Evolution, Issue 36/8, 2019, Page(s) 1601-1611, ISSN 0737-4038
Publisher: Oxford University Press
DOI: 10.1093/molbev/msz109

Suboptimal Global Transcriptional Response Increases the Harmful Effects of Loss-of-Function Mutations

Author(s): Károly Kovács; Zoltán Farkas; Djordje Bajić; Dorottya Kalapis; Andreea Daraba; Karola Almási; Bálint Kintses; Bálint Kintses; Zoltán Bódi; Richard A. Notebaart; Juan F. Poyatos; Patrick Kemmeren; Frank C. P. Holstege; Csaba Pál; Balázs Papp
Published in: Molecular Biology and Evolution, 38(3), 1137 - 1150, Issue 2, 2020, ISSN 0024-2160
Publisher: Oxford University Press
DOI: 10.1093/molbev/msaa280

Pathogen diversity drives the evolution of generalist MHC-II alleles in human populations

Author(s): Máté Manczinger, Gábor Boross, Lajos Kemény, Viktor Müller, Tobias L. Lenz, Balázs Papp, Csaba Pál
Published in: PLOS Biology, Issue 17/1, 2019, Page(s) e3000131, ISSN 1545-7885
Publisher: Plos Biology
DOI: 10.1371/journal.pbio.3000131

Phenotypic heterogeneity promotes adaptive evolution

Author(s): Zoltán Bódi, Zoltán Farkas, Dmitry Nevozhay, Dorottya Kalapis, Viktória Lázár, Bálint Csörgő, Ákos Nyerges, Béla Szamecz, Gergely Fekete, Balázs Papp, Hugo Araújo, José L. Oliveira, Gabriela Moura, Manuel A. S. Santos, Tamás Székely Jr, Gábor Balázsi, Csaba Pál
Published in: PLOS Biology, Issue 15/5, 2017, Page(s) e2000644, ISSN 1545-7885
Publisher: Public Library of Science
DOI: 10.1371/journal.pbio.2000644

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

Author(s): Ákos Nyerges, Bálint Csörgő, István Nagy, Balázs Bálint, Péter Bihari, Viktória Lázár, Gábor Apjok, Kinga Umenhoffer, Balázs Bogos, György Pósfai, Csaba Pál
Published in: Proceedings of the National Academy of Sciences, Issue 113/9, 2016, Page(s) 2502-2507, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1520040113

Collateral sensitivity of antibiotic-resistant microbes

Author(s): Csaba Pál, Balázs Papp, Viktória Lázár
Published in: Trends in Microbiology, Issue 23/7, 2015, Page(s) 401-407, ISSN 0966-842X
Publisher: Elsevier BV
DOI: 10.1016/j.tim.2015.02.009

Evolution of complex adaptations in molecular systems

Author(s): Csaba Pál, Balázs Papp
Published in: Nature Ecology & Evolution, Issue 1/8, 2017, Page(s) 1084-1092, ISSN 2397-334X
Publisher: Springer Natur
DOI: 10.1038/s41559-017-0228-1

Adaptive evolution of complex innovations through stepwise metabolic niche expansion

Author(s): Balázs Szappanos, Jonathan Fritzemeier, Bálint Csörgő, Viktória Lázár, Xiaowen Lu, Gergely Fekete, Balázs Bálint, Róbert Herczeg, István Nagy, Richard A. Notebaart, Martin J. Lercher, Csaba Pál, Balázs Papp
Published in: Nature Communications, Issue 7, 2016, Page(s) 11607, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms11607

Indispensability of Horizontally Transferred Genes and Its Impact on Bacterial Genome Streamlining

Author(s): Ildikó Karcagi, Gábor Draskovits, Kinga Umenhoffer, Gergely Fekete, Károly Kovács, Orsolya Méhi, Gabriella Balikó, Balázs Szappanos, Zsuzsanna Györfy, Tamás Fehér, Balázs Bogos, Frederick R. Blattner, Csaba Pál, György Pósfai, Balázs Papp
Published in: Molecular Biology and Evolution, Issue 33/5, 2016, Page(s) 1257-1269, ISSN 0737-4038
Publisher: Oxford University Press
DOI: 10.1093/molbev/msw009

Evolution of Robustness to Protein Mistranslation by Accelerated Protein Turnover

Author(s): Dorottya Kalapis, Ana R. Bezerra, Zoltán Farkas, Peter Horvath, Zoltán Bódi, Andreea Daraba, Béla Szamecz, Ivo Gut, Mónica Bayes, Manuel A. S. Santos, Csaba Pál
Published in: PLOS Biology, Issue 13/11, 2015, Page(s) e1002291, ISSN 1545-7885
Publisher: Public Library of Science
DOI: 10.1371/journal.pbio.1002291

Efflux Pump Control Alters Synthetic Gene Circuit Function

Author(s): Junchen Diao, Daniel A. Charlebois, Dmitry Nevozhay, Zoltán Bódi, Csaba Pál, Gábor Balázsi
Published in: ACS Synthetic Biology, Issue 5/7, 2016, Page(s) 619-631, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.5b00154

Antibiotic-resistant bacteria show widespread collateral sensitivity to antimicrobial peptides

Author(s): Viktória Lázár, Ana Martins, Réka Spohn, Lejla Daruka, Gábor Grézal, Gergely Fekete, Mónika Számel, Pramod K Jangir, Bálint Kintses, Bálint Csörgő, Ákos Nyerges, Ádám Györkei, András Kincses, András Dér, Fruzsina R Walter, Mária A Deli, Edit Urbán, Zsófia Hegedűs, Gábor Olajos, Orsolya Méhi, Balázs Bálint, István Nagy, Tamás A Martinek, Balázs Papp, Csaba Pál
Published in: Nature Microbiology, Issue 3/6, 2018, Page(s) 718-731, ISSN 2058-5276
Publisher: Nature Research Journals
DOI: 10.1038/s41564-018-0164-0

Directed evolution of multiple genomic loci allows the prediction of antibiotic resistance

Author(s): Ákos Nyerges, Bálint Csörgő, Gábor Draskovits, Bálint Kintses, Petra Szili, Györgyi Ferenc, Tamás Révész, Eszter Ari, István Nagy, Balázs Bálint, Bálint Márk Vásárhelyi, Péter Bihari, Mónika Számel, Dávid Balogh, Henrietta Papp, Dorottya Kalapis, Balázs Papp, Csaba Pál
Published in: Proceedings of the National Academy of Sciences, Issue 115/25, 2018, Page(s) E5726-E5735, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1801646115

Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations

Author(s): Petra Szili, Gábor Draskovits, Tamás Révész, Ferenc Bogár, Dávid Balogh, Tamás Martinek, Lejla Daruka, Réka Spohn, Bálint Márk Vásárhelyi, Márton Czikkely, Bálint Kintses, Gábor Grézal, Györgyi Ferenc, Csaba Pál, Ákos Nyerges
Published in: Antimicrobial Agents and Chemotherapy, Issue 63/9, 2019, ISSN 0066-4804
Publisher: American Society for Microbiology
DOI: 10.1128/aac.00207-19

Hsp70-associated chaperones have a critical role in buffering protein production costs

Author(s): Zoltán Farkas, Dorottya Kalapis, Zoltán Bódi, Béla Szamecz, Andreea Daraba, Karola Almási, Károly Kovács, Gábor Boross, Ferenc Pál, Péter Horváth, Tamás Balassa, Csaba Molnár, Aladár Pettkó-Szandtner, Éva Klement, Edit Rutkai, Attila Szvetnik, Balázs Papp, Csaba Pál
Published in: eLife, Issue 7, 2018, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.29845

Rapid decline of bacterial drug-resistance in an antibiotic-free environment through phenotypic reversion

Author(s): Anett Dunai, Réka Spohn, Zoltán Farkas, Viktória Lázár, Ádám Györkei, Gábor Apjok, Gábor Boross, Balázs Szappanos, Gábor Grézal, Anikó Faragó, László Bodai, Balázs Papp, Csaba Pál
Published in: eLife, Issue 8, 2019, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.47088

Chemical-genetic profiling reveals limited cross-resistance between antimicrobial peptides with different modes of action

Author(s): Bálint Kintses, Pramod K. Jangir, Gergely Fekete, Mónika Számel, Orsolya Méhi, Réka Spohn, Lejla Daruka, Ana Martins, Ali Hosseinnia, Alla Gagarinova, Sunyoung Kim, Sadhna Phanse, Bálint Csörgő, Ádám Györkei, Eszter Ari, Viktória Lázár, István Nagy, Mohan Babu, Csaba Pál, Balázs Papp
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-13618-z

Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene

Author(s): Tomas Aparicio, Akos Nyerges, István Nagy, Csaba Pal, Esteban Martínez‐García, Víctor Lorenzo
Published in: Environmental Microbiology, Issue 22/1, 2019, Page(s) 45-58, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14814

Chemical-genetic profiling to investigate cross-resistance and collateral sensitivity between antimicrobial peptides

Author(s): Kintses, Bálint; Jangir, Pramod K.; Fekete, Gergely; Számel, Mónika; Méhi, Orsolya; Spohn, Réka; Daruka, Lejla; Martins, Ana; Hosseinnia, Ali; Gagarinova, Alla; Kim, Sunyoung; Phanse, Sadhna; Csörgő, Bálint; Györkei, Ádám; Ari, Eszter; Lázár, Viktória; Faragó, Anikó; Bodai, László; Nagy, István; Babu, Mohan; Pál, Csaba; Papp, Balázs
Published in: Nature Communications, Issue 3, 2019, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1101/542548