Tailored Elastin-like Recombinamers as Advanced Systems for Cell Therapies in Diabetes Mellitus: a Synthetic Biology Approach towards a Bioeffective and Immunoisolated Biosimilar Islet/Cell Niche

Project Information

ELASTISLET

Grant agreement ID: 646075

DOI 10.3030/646075

Project closed

EC signature date 28 May 2015

Start date 1 June 2015

End date 31 May 2019

Funded under

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Advanced materials

Total cost € 6 214 495,00

EU contribution € 6 214 495,00

Coordinated by

UNIVERSIDAD DE VALLADOLID
Spain

Deliverables

Documents, reports (4)

Quality and contingency

A quality and contingency plan is ready to be distributed

Report and management activities

A report on data mangement activities will be published

Communication and dissemination plan

A well-defined Communication and Dissemination plan, including communication objectives, messages, target audience and the tools/methods to reach the widest audience, will be defined and published. The Project image will be presented by month 6 The communication and dissemination activities carried out along the Project life, including Scientific conferences and publications, will be published by month 48

Report on existing standards and standardization landscape

A report describing the existing standards and standardization landscape will be published

Websites, patent fillings, videos etc. (1)

Project website

The Project website will be designed and ready to work

Publications

Peer reviewed articles (41)

It Takes Two to Tango: Coupling of Angiogenesis and Osteogenesis for Bone Regeneration

Author(s): Andrea Grosso, Maximilian G. Burger, Alexander Lunger, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Published in: Frontiers in Bioengineering and Biotechnology, Issue 5, 2017, Page(s) 68, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2017.00068

Biomimetic click assembled multilayer coatings exhibiting responsive properties

Author(s): Maria P. Sousa, Israel Gonzalez de Torre, Mariana B. Oliveira, José C. Rodríguez-Cabello, João F. Mano
Published in: Materials Today Chemistry, Issue 4, 2017, Page(s) 150-163, ISSN 2468-5194
Publisher: Elsevier Ltd.
DOI: 10.1016/j.mtchem.2017.04.001

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

Author(s): Sebastian Manuel Staubli, Giulia Cerino, Israel Gonzalez De Torre, Matilde Alonso, Daniel Oertli, Friedrich Eckstein, Katharina Glatz, José Carlos Rodríguez Cabello, Anna Marsano
Published in: Biomaterials, Issue 135, 2017, Page(s) 30-41, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2017.04.047

Bioactive scaffolds based on elastin-like materials for wound healing

Author(s): J. Carlos Rodríguez-Cabello, I. González de Torre, A. Ibañez-Fonseca, M. Alonso
Published in: Advanced Drug Delivery Reviews, Issue 129, 2018, Page(s) 118-133, ISSN 0169-409X
Publisher: Elsevier BV
DOI: 10.1016/j.addr.2018.03.003

Beta Cell Death by Cell-free DNA and Outcome After Clinical Islet Transplantation

Author(s): Boris L. Gala-Lopez, Daniel Neiman, Tatsuya Kin, Doug O’Gorman, Andrew R. Pepper, Andrew J. Malcolm, Sheina Pianzin, Peter A. Senior, Patricia Campbell, Benjamin Glaser, Yuval Dor, Ruth Shemer, A.M. James Shapiro
Published in: Transplantation, Issue 102/6, 2018, Page(s) 978-985, ISSN 0041-1337
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/TP.0000000000002083

Biocompatibility of two model elastin-like recombinamer-based hydrogels formed through physical or chemical cross-linking for various applications in tissue engineering and regenerative medicine

Author(s): Arturo Ibáñez-Fonseca, Teresa L. Ramos, Israel González de Torre, Luis Ignacio Sánchez-Abarca, Sandra Muntión, Francisco Javier Arias, María Consuelo del Cañizo, Matilde Alonso, Fermín Sánchez-Guijo, José Carlos Rodríguez-Cabello
Published in: Journal of Tissue Engineering and Regenerative Medicine, Issue 12/3, 2018, Page(s) e1450-e1460, ISSN 1932-6254
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/term.2562

Random and oriented electrospun fibers based on a multicomponent, in situ clickable elastin-like recombinamer system for dermal tissue engineering

Author(s): Israel González de Torre, Arturo Ibáñez-Fonseca, Luis Quintanilla, Matilde Alonso, José-Carlos Rodríguez-Cabello
Published in: Acta Biomaterialia, Issue 72, 2018, Page(s) 137-149, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2018.03.027

Islet Encapsulation: Physiological Possibilities and Limitations

Author(s): Olle Korsgren
Published in: Diabetes, Issue 66/7, 2017, Page(s) 1748-1754, ISSN 0012-1797
Publisher: American Diabetes Association
DOI: 10.2337/db17-0065

Transplantation of macroencapsulated human islets within the bioartificial pancreas βAir to patients with type 1 diabetes mellitus

Author(s): Per-Ola Carlsson, Daniel Espes, Amir Sedigh, Avi Rotem, Baruch Zimerman, Helena Grinberg, Tali Goldman, Uriel Barkai, Yuval Avni, Gunilla T. Westermark, Lina Carlbom, Håkan Ahlström, Olof Eriksson, Johan Olerud, Olle Korsgren
Published in: American Journal of Transplantation, Issue 18/7, 2018, Page(s) 1735-1744, ISSN 1600-6135
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/ajt.14642

Glucose Effectiveness

Author(s): Erik Korsgren, Olle Korsgren
Published in: Transplantation, Issue 100/1, 2016, Page(s) 111-115, ISSN 0041-1337
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/TP.0000000000000900

Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants

Author(s): Jeevan Govindharajulu, Xi Chen, Yuping Li, Jose Rodriguez-Cabello, Mrinal Battacharya, Conrado Aparicio
Published in: International Journal of Molecular Sciences, Issue 18/2, 2017, Page(s) 369, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms18020369

Förster Resonance Energy Transfer-Paired Hydrogel Forming Silk-Elastin-Like Recombinamers by Recombinant Conjugation of Fluorescent Proteins

Author(s): Arturo Ibáñez-Fonseca, Matilde Alonso, Francisco Javier Arias, José Carlos Rodríguez-Cabello
Published in: Bioconjugate Chemistry, Issue 28/3, 2017, Page(s) 828-835, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.6b00738

Intrafibrillar Mineralization of Self-Assembled Elastin-Like Recombinamer Fibrils

Author(s): Yuping Li, Jose Carlos Rodriguez-Cabello, Conrado Aparicio
Published in: ACS Applied Materials & Interfaces, Issue 9/7, 2017, Page(s) 5838-5846, ISSN 1944-8244
Publisher: American Chemical Society
DOI: 10.1021/acsami.6b15285

Cross-linking of a biopolymer-peptide co-assembling system

Author(s): Karla E. Inostroza-Brito, Estelle C. Collin, Anna Majkowska, Sherif Elsharkawy, Alistair Rice, Armando E. del Río Hernández, Xin Xiao, José Rodríguez-Cabello, Alvaro Mata
Published in: Acta Biomaterialia, Issue 58, 2017, Page(s) 80-89, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2017.05.043

Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend

Author(s): Esen Sayin, Rosti Hama Rashid, José Carlos Rodríguez-Cabello, Ahmed Elsheikh, Erkan Türker Baran, Vasif Hasirci
Published in: Bioactive Materials, Issue 2/2, 2017, Page(s) 71-81, ISSN 2452-199X
Publisher: Royal Society of Chemistry
DOI: 10.1016/j.bioactmat.2017.04.001

Recombinant DNA technology and click chemistry: a powerful combination for generating a hybrid elastin-like-statherin hydrogel to control calcium phosphate mineralization

Author(s): Mohamed Hamed Misbah, Mercedes Santos, Luis Quintanilla, Christina Günter, Matilde Alonso, Andreas Taubert, José Carlos Rodríguez-Cabello
Published in: Beilstein Journal of Nanotechnology, Issue 8, 2017, Page(s) 772-783, ISSN 2190-4286
Publisher: Beilstein-Institut Zur Forderung der Chemischen Wissenschaften
DOI: 10.3762/bjnano.8.80

Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels

Author(s): David Pescador, Arturo Ibáñez-Fonseca, Fermín Sánchez-Guijo, Jesús G. Briñón, Francisco Javier Arias, Sandra Muntión, Cristina Hernández, Alessandra Girotti, Matilde Alonso, María Consuelo del Cañizo, José Carlos Rodríguez-Cabello, Juan Francisco Blanco
Published in: Journal of Materials Science: Materials in Medicine, Issue 28/8, 2017, Page(s) 115, ISSN 0957-4530
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10856-017-5928-1

PHBV wet spun scaffold coated with ELR-REDV improves vascularization for bone tissue engineering

Author(s): Ayse Selcen Alagoz, Jose Carlos Rodriguez-Cabello, Vasif Hasirci
Published in: Biomedical Materials, 2018, ISSN 1748-6041
Publisher: Institute of Physics Publishing
DOI: 10.1088/1748-605X/aad139

Protein disorder–order interplay to guide the growth of hierarchical mineralized structures

Author(s): Sherif Elsharkawy, Maisoon Al-Jawad, Maria F. Pantano, Esther Tejeda-Montes, Khushbu Mehta, Hasan Jamal, Shweta Agarwal, Kseniya Shuturminska, Alistair Rice, Nadezda V. Tarakina, Rory M. Wilson, Andy J. Bushby, Matilde Alonso, Jose C. Rodriguez-Cabello, Ettore Barbieri, Armando del Río Hernández, Molly M. Stevens, Nicola M. Pugno, Paul Anderson, Alvaro Mata
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04319-0

Tuning the stiffness of surfaces by assembling genetically engineered polypeptides with tailored amino acid sequence

Author(s): Rui R. Costa, Miguel González-Pérez, Marcos Herrero-Gutiérrez, Ricardo A. Pires, Matilde Alonso, J. Carlos Rodriguez-Cabello, Rui L. Reis, Iva Pashkuleva
Published in: Biomacromolecules, 2018, Page(s) 3401-3411, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.8b00723

Macroporous click-elastin-like hydrogels for tissue engineering applications

Author(s): Alicia Fernández-Colino, Frederic Wolf, Hans Keijdener, Stephan Rütten, Thomas Schmitz-Rode, Stefan Jockenhoevel, J. Carlos Rodríguez-Cabello, Petra Mela
Published in: Materials Science and Engineering: C, Issue 88, 2018, Page(s) 140-147, ISSN 0928-4931
Publisher: Elsevier BV
DOI: 10.1016/j.msec.2018.03.013

Cartilage Regeneration in Preannealed Silk Elastin-Like Co-Recombinamers Injectable Hydrogel Embedded with Mature Chondrocytes in an Ex Vivo Culture Platform

Author(s): Filippo Cipriani, Melanie Krüger, Israel Gonzalez de Torre, Luis Quintanilla Sierra, Matilde Alonso Rodrigo, Linda Kock, José Carlos Rodriguez-Cabello
Published in: Biomacromolecules, Issue 19/11, 2018, Page(s) 4333-4347, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.8b01211

A transferrin receptor-binding mucoadhesive elastin-like recombinamer: In vitro and in vivo characterization

Author(s): Constancio Gonzalez-Obeso, Alessandra Girotti, J. Carlos Rodriguez-Cabello
Published in: Acta Biomaterialia, Issue 88, 2019, Page(s) 241-250, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2019.02.027

Bicyclic RGD peptides with high integrin α v β 3 and α 5 β 1 affinity promote cell adhesion on elastin-like recombinamers

Author(s): Filippo Cipriani, Dominik Bernhagen, Carmen García-Arévalo, Israel González de Torre, Peter Timmerman, José Carlos Rodríguez-Cabello
Published in: Biomedical Materials, Issue 14/3, 2019, Page(s) 035009, ISSN 1748-605X
Publisher: IOP Publishing
DOI: 10.1088/1748-605x/aafd83

Bioinspired baroplastic glycosaminoglycan sealants for soft tissues

Author(s): Rui R. Costa, Diana Soares da Costa, Rui L. Reis, Iva Pashkuleva
Published in: Acta Biomaterialia, Issue 87, 2019, Page(s) 108-117, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2019.01.040

Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers

Author(s): Tatjana Flora, I. González de Torre, Luis Quintanilla, M. Alonso, J. Carlos Rodríguez-Cabello
Published in: European Polymer Journal, Issue 106, 2018, Page(s) 19-29, ISSN 0014-3057
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.eurpolymj.2018.06.026

A novel lipase-catalyzed method for preparing ELR-based bioconjugates

Author(s): Ana M. Testera, Mercedes Santos, Alessandra Girotti, F. Javier Arias, J. Manuel Báñez, Matilde Alonso, J. Carlos Rodríguez-Cabello
Published in: International Journal of Biological Macromolecules, Issue 121, 2019, Page(s) 752-759, ISSN 0141-8130
Publisher: Elsevier BV
DOI: 10.1016/j.ijbiomac.2018.10.028

Tethering QK peptide to enhance angiogenesis in elastin-like recombinamer (ELR) hydrogels

Author(s): Tatjana Flora, I. González de Torre, M. Alonso, J. Carlos Rodríguez-Cabello
Published in: Journal of Materials Science: Materials in Medicine, Issue 30/2, 2019, ISSN 0957-4530
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10856-019-6232-z

Coating Strategies Using Layer-by-layer Deposition for Cell Encapsulation

Author(s): Mariana B. Oliveira, Javad Hatami, João F. Mano
Published in: Chemistry - An Asian Journal, Issue 11/12, 2016, Page(s) 1753-1764, ISSN 1861-4728
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/asia.201600145

Co-assembly, spatiotemporal control and morphogenesis of a hybrid protein–peptide system

Author(s): Karla E. Inostroza-Brito, Estelle Collin, Orit Siton-Mendelson, Katherine H. Smith, Amàlia Monge-Marcet, Daniela S. Ferreira, Raúl Pérez Rodríguez, Matilde Alonso, José Carlos Rodríguez-Cabello, Rui L. Reis, Francesc Sagués, Lorenzo Botto, Ronit Bitton, Helena S. Azevedo, Alvaro Mata
Published in: Nature Chemistry, Issue 7/11, 2015, Page(s) 897-904, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/nchem.2349

Multilayered Films Produced by Layer-by-Layer Assembly of Chitosan and Alginate as a Potential Platform for the Formation of Human Adipose-Derived Stem Cell aggregates

Author(s): Javad Hatami, Sandra Silva, Mariana Oliveira, Rui Costa, Rui Reis, João Mano
Published in: Polymers, Issue 9/12, 2017, Page(s) 440, ISSN 2073-4360
Publisher: MPDI Open Access Publishing
DOI: 10.3390/polym9090440

3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine

Author(s): Mercedes Vila, Ana García, Alessandra Girotti, Matilde Alonso, Jose Carlos Rodríguez-Cabello, Arlyng González-Vázquez, Josep A. Planell, Elisabeth Engel, Julia Buján, Natalio García-Honduvilla, María Vallet-Regí
Published in: Acta Biomaterialia, Issue 45, 2016, Page(s) 349-356, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2016.09.016

Genetically Engineered Elastin-based Biomaterials for Biomedical Applications

Author(s): Mercedes Santos, Sofia Serrano-Ducar, Juan Gonzalez-Valdivieso, Reinaldo Vallejo, Alessandra Girotti, Purificacion Cuadrado, Francisco Javier Arias
Published in: Current Medicinal Chemistry, Issue 25, 2018, Page(s) 1-28, ISSN 0929-8673
Publisher: Bentham Science Publishers
DOI: 10.2174/0929867325666180508094637

Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA

Author(s): Hai Zemmour, David Planer, Judith Magenheim, Joshua Moss, Daniel Neiman, Dan Gilon, Amit Korach, Benjamin Glaser, Ruth Shemer, Giora Landesberg, Yuval Dor
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-03961-y

Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease

Author(s): Joshua Moss, Judith Magenheim, Daniel Neiman, Hai Zemmour, Netanel Loyfer, Amit Korach, Yaacov Samet, Myriam Maoz, Henrik Druid, Peter Arner, Keng-Yeh Fu, Endre Kiss, Kirsty L. Spalding, Giora Landesberg, Aviad Zick, Albert Grinshpun, A. M. James Shapiro, Markus Grompe, Avigail Dreazan Wittenberg, Benjamin Glaser, Ruth Shemer, Tommy Kaplan, Yuval Dor
Published in: Nature Communications, Issue 9/1, 2018, Page(s) 1-12, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-07466-6

Self-assembly in Elastin-Like Recombinamers: a Mechanism to Mimic Natural Complexity

Author(s): Luis Quintanilla-Sierra, Carmen García-Arévalo, J.C. Rodriguez-Cabello
Published in: Materials Today Bio, 2019, Page(s) 100007, ISSN 2590-0064
Publisher: Elsevier
DOI: 10.1016/j.mtbio.2019.100007

Monitoring liver damage using hepatocyte-specific methylation markers in cell-free circulating DNA

Author(s): Roni Lehmann-Werman, Judith Magenheim, Joshua Moss, Daniel Neiman, Ofri Abraham, Sheina Piyanzin, Hai Zemmour, Ilana Fox, Talya Dor, Markus Grompe, Giora Landesberg, Bao-Li Loza, Abraham Shaked, Kim Olthoff, Benjamin Glaser, Ruth Shemer, Yuval Dor
Published in: JCI Insight, Issue 3/12, 2018, ISSN 2379-3708
Publisher: JCI Insight
DOI: 10.1172/jci.insight.120687

Hydrophobic Cholesteryl Moieties Trigger Substrate Cell–Membrane Interaction of Elastin–Mimetic Protein Coatings in Vitro

Author(s): Leander Poocza, Filippo Cipriani, Matilde Alonso, José Carlos Rodríguez-Cabello
Published in: ACS Omega, Issue 4/6, 2019, Page(s) 10818-10827, ISSN 2470-1343
Publisher: ACS Publications
DOI: 10.1021/acsomega.9b00548

Use of proteolytic sequences with different cleavage kinetics as a way to generate hydrogels with preprogrammed cell-infiltration patterns imparted over their given 3D spatial structure

Author(s): Tatjana Flora, I González de Torre, M Alonso, J Carlos Rodríguez-Cabello
Published in: Biofabrication, Issue 11/3, 2019, Page(s) 035008, ISSN 1758-5090
Publisher: IOP Publishing
DOI: 10.1088/1758-5090/ab10a5

Self-Assembling ELR-Based Nanoparticles as Smart Drug-Delivery Systems Modulating Cellular Growth via Akt

Author(s): Juan Gonzalez-Valdivieso, Alessandra Girotti, Raquel Muñoz, J. Carlos Rodriguez-Cabello, F. Javier Arias
Published in: Biomacromolecules, Issue 20/5, 2019, Page(s) 1996-2007, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.9b00206

Silk-ELR co-recombinamer covered stents obtained by electrospinning

Author(s): M Putzu, F Causa, Manuel Parente, Israel González de Torre, J C Rodriguez-Cabello, P A Netti
Published in: Regenerative Biomaterials, Issue 6/1, 2018, Page(s) 21-28, ISSN 2056-3418
Publisher: ACS Publications
DOI: 10.1093/rb/rby022