Development of novel monitoring devices to detect microbial contaminants in foods during processing and storage

Project Information

Grant agreement ID: AGRF0049

Project closed

Start date 1 March 1991

End date 28 February 1995

Funded under

• Multiannual research and development programme (EEC) in food science and technology (FLAIR), 1989-1993

Total cost No data

EU contribution No data

Coordinated by

National University of Ireland, Cork
Ireland

Objective

This project sets out to develop a novel prototype acoustic biosensor for use in the detection of microbial pathogens and spoilage organisms in food.
Research has been carried out in order to exploit the aspect of immunological specificity coupled to acoustic based transducer technology for the detection of spoilage/pathogenic microorganisms in food.

The work has involved:
the nucleotide sequence determination of a ferric siderophore receptor protein from Pseudomonas strain M114 domain analysis of the receptor protein to identify surface exposed regions and to generate antibodies directed agaisnt such regions;
analysis of the adsorption of radiolabelled immunoglobulin G (IgG) to silver and lithium niobate surfaces;
fabrication, electrical characterization and analysis of the performance of surface acoustic wave (SAW) devices in liquid environments;
design and construction of SAW devices;
assessment of the specificity of developed antibodies.
Biotechnology and microelectronics are brought together in this pre-competitive project. The project is concerned with:

1. Identification of novel surface antigens for the detection of particular strains/species of bacteria;

2. Development of poly- and monoclonal antibodies to these surface antigens;

3. Evaluation of these developed antibodies with regard to species/strain specificity;

4. Acoustic wave transducer design for biosensor applications;

5. Modelling of surface acoustic wave (SAW) transducer devices;

6. Definition, development and optimisation of an acoustic transducer fabrication process;

7. Development of interfacial chemistries to covalently attach antibodies to the transducer surface;

8. Comparison of the newly developed prototype acoustic biosensor with the available technologies for the detection of pathogenic and spoilage bacteria in food.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• engineering and technology › electrical engineering, electronic engineering, information engineering › electronic engineering › sensors › biosensors
• natural sciences › biological sciences › microbiology › bacteriology
• natural sciences › chemical sciences › inorganic chemistry › alkali metals
• natural sciences › chemical sciences › inorganic chemistry › transition metals
• natural sciences › biological sciences › biochemistry › biomolecules › proteins

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Programme(s)

• FP2-FLAIR - Multiannual research and development programme (EEC) in food science and technology (FLAIR), 1989-1993

Topic(s)

Data not available

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

National University of Ireland, Cork

• EU contribution: No data
• Address: Western Road ; 30 Cork ; Ireland
• Total cost: No data

Participants (2)

Quality Assurance Laboratory

Ireland

• EU contribution: No data
• Address: Moorepark ; Fermoy
• Total cost: No data

University of Cambridge

United Kingdom

• EU contribution: No data
• Address: Tennis Court Road ; CB2 1QT Cambridge
• Total cost: No data