Descrizione del progetto
Un saggio 3 in 1 per i test di cardiotossicità
Le cellule staminali pluripotenti indotte (iPSC, induced Pluripotent Stem Cells) umane hanno rivoluzionato lo sviluppo di modelli di organi e malattie in vitro. Allo stesso tempo, hanno evidenziato la necessità di letture più sensibili, informative e accurate del comportamento specifico delle cellule e della funzione anomala. Il progetto ACQUIRE, finanziato dall’UE, concentra l’attenzione su strumenti per la valutazione della funzionalità dei cardiomiociti derivati da iPSC umane. L’obiettivo principale è quello di portare sul mercato il sistema di misurazione a triplo transiente (TTM, Triple Transient Measurement) precedentemente sviluppato. Il sistema TTM quantifica contemporaneamente l’attività elettrica, il flusso intracellulare di calcio e la contrattilità, offrendo uno strumento multimodale per la valutazione in vitro della tossicità dei farmaci su modelli di cardiomiociti derivati da iPSC umane.
Obiettivo
"Academia and industry urgently needs reliable models to study heart failure and toxic effects of drugs on the heart. While new models based on human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are now emerging, accurate readouts of cardiomyocyte function fall short of needs. Apart from improving the models biologically, more sensitive, informative and accurate readouts are needed to detect abnormal cardiomyocyte behaviour. Several tools have proven their ability to assess electrical changes or calcium handling in hiPSC-CMs, but they are typically incompatible with 3D tissue models and moreover, there is paucity of appropriate tools to quantify the most important function of myocardium: contraction. Our ERC Advanced Grant STEMCARDIOVASC entailed the development of improved tools for cardiac functionality. One of the most important bioassays developed as an outcome of STEMCARDIOVASC was the Triple Transient Measurement (TTM) System. The TTM System quantifies electrical activity, intracellular calcium flux and contractility simultaneously and is our answer to the challenge of pharma in understanding when and how drugs or diseases affect cardiac contractility using hiPSC-CM models. In this ERC Proof of Concept project “ACQUIRE”, we strive to bring the TTM to a commercial applicable service, and later product. To reach this goal we have set out four aims to come to a Minimum Viable Product: i) increase the flexibility of the system to accommodate a larger variety of optical probes, ii) increase the throughput of the system to compete with current measurement systems, iii) increase user friendliness by integrating software modules for running and analysing measurements and iv) define a route for commercialisation.
Resulting from ""ACQUIRE"" the TTM System can be commercialized as a human cardiac based 3-in-1 assay for cardiotoxicity testing and a novel tool for providing mechanistic insight in the EC coupling for disease modelling and drug discovery."
Campo scientifico
Not validated
Not validated
- medical and health sciencesbasic medicinepharmacology and pharmacydrug discovery
- natural sciencescomputer and information sciencessoftware
- natural scienceschemical sciencesinorganic chemistryalkaline earth metals
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- medical and health sciencesclinical medicinecardiology
Programma(i)
Argomento(i)
Meccanismo di finanziamento
ERC-POC - Proof of Concept GrantIstituzione ospitante
2333 ZA Leiden
Paesi Bassi