Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Livrables

Integrated system for calibration and testing
First prototypes of climate chamber module and liquid handling module and specification for the automated system

Realization of the first prototypes of climate chamber module and liquid handling module and specification for the automated system

First prototype of the three individual layers for successive full integration

Realization of the first prototype of the three individual layers for successive full integration of the super-sensor.

Logo and webpage

Realization of the logo of the Project and of a dedicated website with domain .eu in the address.

Flyers with project targets and first results and promotional movie

Flyers which describe the project targets and the first results achieved. Movie which explains the aims of the Project and its impact in order to disseminate as best as possible the research activities.

International Symposium

Organization of an International Symposium involving the main Universities and Research Centers and Companies working in the field of early diagnosis in neurodegenerative diseases but also on fields regarding microscale fluid handling and immunoreactions at sub-microliter levels.

Final plan for dissemination and exploitation

Report on the final plan for dissemination and exploitation of the results

Technical/scientific review meeting 1 documents

Document delivered as first report with a summary on the work in progress and on the use of resources including those regarding the dissemination activities

Report on sensitivity and specificity for tau

Report containing the data obtained for the sensitivity and the specificity of the supersensor for the biomarker tau

Report on sensitivity and specificity for Abeta1-42

Report containing the data concerning the results on the sensitivity and specificity of the supersensor for the biomarker Abeta142

Report on sensitivity and specificity for P-tau

Report document containing the data obtained for the sensitivity and the specificity of the supersensor for the biomarker Ptau

Publications

High‐Rate Accumulation of Tiny Aqueous Droplets Using a Pyroelectrohydrodynamic Jet System

Auteurs: Volodymyr Tkachenko, Reinhard Schwödiauer, Romina Rega, Simona Itri, Martina Mugnano, Danila del Giudice, Heidi Ottevaere, Yunfeng Nie, Pietro Ferraro, Simonetta Grilli
Publié dans: Advanced Engineering Materials, 2021, Page(s) 2100756, ISSN 1438-1656
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/adem.202100756

Direct printing of gold nanospheres from colloidal solutions by pyro-electrohydrodynamic jet allows hypersensitive SERS sensing

Auteurs: Marianna Pannico, Pellegrino Musto, Romina Rega, Veronica Vespini, Simonetta Grilli, Pietro Ferraro
Publié dans: Applied Surface Science, Numéro 531, 2020, Page(s) 147393, ISSN 0169-4332
Éditeur: Elsevier BV
DOI: 10.1016/j.apsusc.2020.147393

Miniaturized, high numerical aperture confocal fluorescence detection enhanced with pyroelectric droplet accumulation for sub-attomole analyte diagnosis

Auteurs: YUNFENG NIE, UUSITALO SANNA, TEEMU SIPOLA, ANNUKKA KOKKONEN, INKA PÄKKILÄ, JUHA SUMEN, KATARIINA RAHKAMAA-TOLONEN, VOLODYMYR TKACHENKO, VERONICA VESPINI, SARA COPPOLA, PIETRO FERRARO, SIMONETTA GRILLI, HEIDI OTTEVAERE
Publié dans: Biomedical Optics Express, 2023, ISSN 2156-7085
Éditeur: The Optical Society
DOI: 10.1364/boe.504757

High-accuracy characterization of pyroelectric materials: A noncontact method based on surface potential measurements

Auteurs: Reinhard Schwödiauer, Simona Bauer-Gogonea, Martin Kaltenbrunner, Volodymyr Tkachenko, Simonetta Grilli
Publié dans: JOURNAL OF ADVANCED DIELECTRICS, 2023, ISSN 2010-1368
Éditeur: World Scientific
DOI: 10.1142/s2010135x23410023

Rapid Formation of Self‐Supporting Polydimethylsiloxane Sheets with Periodic Clusters of Embedded Nickel Nanoparticles

Auteurs: Romina Rega, Juan Francisco Muñoz Martinez, Vito Pagliarulo, Angela Longo, Mariano Palomba, Gianfranco Carotenuto, Pietro Ferraro, Simonetta Grilli
Publié dans: Advanced Materials Interfaces, 2021, Page(s) 2002216, ISSN 2196-7350
Éditeur: Wiley-VCH GmbH
DOI: 10.1002/admi.202002216

Cellular Uptake of Mildly Oxidized Nanographene for Drug-Delivery Applications

Auteurs: M. Mugnano, G. C. Lama, R. Castaldo, V. Marchesano, F. Merola, D. del Giudice, A. Calabuig, G. Gentile, V. Ambrogi, P. Cerruti, P. Memmolo, V. Pagliarulo, P. Ferraro, S. Grilli
Publié dans: ACS Applied Nano Materials, Numéro 3/1, 2019, Page(s) 428-439, ISSN 2574-0970
Éditeur: ACS Publications
DOI: 10.1021/acsanm.9b02035

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Auteurs: Romina Rega, Martina Mugnano, Emilia Oleandro, Volodymyr Tkachenko, Danila del Giudice, Gianluca Bagnato, Pietro Ferraro, Simonetta Grilli, Sebastiano Gangemi
Publié dans: Sensors, Numéro 20/12, 2020, Page(s) 3567, ISSN 1424-8220
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s20123567

Quantitative determination of rapid biomass formation on pyro-electrifiedpolymer sheets

Auteurs: Emilia Oleandro, Romina Rega, Martina Mugnano, Filomena Nazzaro, Pietro Ferraro, Simonetta Grilli
Publié dans: Biofilm, Numéro 3, 2021, Page(s) 100040, ISSN 2590-2075
Éditeur: Elsevier
DOI: 10.1016/j.bioflm.2020.100040

Highly sensitive detection of the neurodegenerative biomarker Tau by using the concentration effect of the pyro-electrohydrodynamic jetting

Auteurs: Concetta Di Natale, Sara Coppola, Veronica Vespini, Volodymyr Tkachenko, Simone Russo, Giuseppina Luciani, Giuseppe Vitiello, Francesca Ferranti, Silvia Mari, Pietro Ferraro, Pier Luca Maffettone, Simonetta Grilli
Publié dans: Biosensors and bioelectronics, 2024, ISSN 0956-5663
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2024.116234

Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro

Auteurs: Romina Rega, Oriella Gennari, Laura Mecozzi, Vito Pagliarulo, Martina Mugnano, Emilia Oleandro, Filomena Nazzaro, Pietro Ferraro, Simonetta Grilli
Publié dans: Frontiers in Chemistry, Numéro 7, 2019, ISSN 2296-2646
Éditeur: Frontiers
DOI: 10.3389/fchem.2019.00429

A pin-based pyro-electrohydrodynamic jet sensor for tuning theaccumulation of biomolecules down to sub-picogram level detection

Auteurs: The detection of low abundant biomarkers is of great interest in different clinical cases, such as the early diagnosis of Alzheimer's disease. Here we present what we call pin-based pyro-electrohydrodynamic jet (p-jet) sensor that can linearly tune the accumulation rate of biomolecules in tiny droplets. In this newly revealed pinbased configuration we demonstrate the ability to detect biomolecules
Publié dans: Sensing and Bio-Sensing Research, Numéro 22141804, 2022, ISSN 2214-1804
Éditeur: Elsevier BV
DOI: 10.1016/j.sbsr.2022.100536

Dispensing pico droplets by pyroelectrohydrodynamic jetting: simulations and experimental results

Auteurs: Anna Faraone
Publié dans: 2019
Éditeur: Zendo
DOI: 10.5281/zenodo.3530462

Numerical simulations of droplet dynamics through an orifice – pin system for pyroelectrodynamic shooting

Auteurs: Pentella Domenico
Publié dans: 2020
Éditeur: Zenodo
DOI: 10.5281/zenodo.4767244

SensApp: a FET-open project for developing a supersensor able to detect Alzheimer's disease biomarkers in blood

Auteurs: Romina Rega, Martina Mugnano, Danila del Giudice, Simona Itri, Volodymyr Tkachenko, Veronica Vespini, Sara Coppola, Pietro Ferraro, Heidi Ottevaere, Yunfeng Nie, Sanna Uusitalo, Reinhard Schwoediauer, Martin Kaltenbrunner, Markku Känsäkoski, Emanuela Mazzon, Pier Luca Maffettone, Gaetano D'Avino, Simonetta Grilli
Publié dans: Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 2021, Page(s) 58, ISBN 9781510644076
Éditeur: SPIE
DOI: 10.1117/12.2596966

Highly sensitive detection of low abundant molecules by pyro-electrohydro-dynamic jetting

Auteurs: ROMINA REGA, Martina Mugnano, Danila Del Giudice, Simona Itri, Volodymyr Tkachenko, veronica Vespini, Sara Coppola, Emanuela Mazzon, Emilia Oleandro, Pietro Ferraro, Simonetta Grilli
Publié dans: Biophotonics in Point-of-Care, 2020, Page(s) 38, ISBN 9781510634954
Éditeur: SPIE
DOI: 10.1117/12.2564859

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