The Extracellular Vesicle Foundry

Project Information

evFOUNDRY

Grant agreement ID: 801367

DOI 10.3030/801367

Project closed

EC signature date 25 May 2018

Start date 1 September 2018

End date 28 February 2022

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost € 2 726 516,25

EU contribution € 2 726 516,25

Coordinated by

CONSORZIO INTERUNIVERSITARIO PERLO SVILUPPO DEI SISTEMI A GRANDE INTERFASE
Italy

Deliverables

Documents, reports (11)

Dissemination and communication plan

Dissemination and communication plan prepared

Optimal storage and shipment of nanosized EVs

Definition and report on optimal storage and shipment of nanosized EVs

SOPs for quality standard nanosized EVs from bovine milk

Standard Operating Procedures (SOPs) for quality standard nanosized EVs from bovine milk defined.

Physical properties of EVs from the prototype benchmarked. Off-line testing.

Physiscochemical properties of EVs produced by the prototype benchmarked Offline testing

Colloidal characterization of nanosized EVs

Report on colloidal characterization of nanosized EVs

EV-surface interaction fundamentals

Report on EVsurface interaction fundamentals

First yearly dissemination and communication report

First annual dissemination and communication report

SOPs for quality standard nanosized EVs from Ascaris media

Standard Operating Procedures (SOPs) for quality standard nanosized EVs from Ascaris media defined.

Modulatory properties of EVs from the prototype benchmarked

Modulatory potential of EVs produced by the prototype benchmarked

Second yearly dissemination and communication report
Feasibility of large scale manufacturing of immune modulatory EVs

Report on feasibility including performance of large scale manufacturing immune modulatory EVs

Other (3)

First public report published

Final public report on the project progress.

Organization of a dedicated summer/winter school

Organisation of a dedicated summer/winter school on the project interdisciplinary themes. Teachers will be both from the consortium and external. Open to undergraduate and PHD students.

Final public report published

Final public report on the project progress

Demonstrators, pilots, prototypes (5)

Library of nano-DLD surfaces

Library of nano-DLD surfaces fabricated.

Library of nanostructured surfaces non-lamellar lipid phases

Library of nanostructured surfaces made of non-lamellar lipid phases fabricated.

Large-scale production prototype assembled
Library of nanostructured surfaces classified by EV interactions

Library of relevant nanostructured surfaces classified in terms of their interactions with the EVs

Integrated modular system for production of EVs

Proof-of-concept integrated modular system assembled

Websites, patent fillings, videos etc. (2)

Internal and main external communication channels

Internal and main external communication channels defined and operating

Project website online and logo

Project website online and logo defined.

Publications

Peer reviewed articles (25)

Comparison of separation methods for immunomodulatory extracellular vesicles from helminths.

Author(s): Borup,A.,Boysen,A.T.,Ridolfi,A.,Brucale,M.,Valle,F.,Paolini,L.,Bergese,P.,&Nejsum,P.
Published in: Journal of Extracellular Biology, Issue 2022;1:e41, 2022, Page(s) 1-17, ISSN 2768-2811
Publisher: John Wiley & Sons, Ltd
DOI: 10.1002/jex2.41

Nanoanalytical analysis of bisphosphonate-driven alterations of microcalcifications using a 3D hydrogel system and in vivo mouse model

Author(s): Ruiz JL, Hutcheson JD, Cardoso L, Bakhshian Nik A, Condado de Abreu A, Pham T, Buffolo F, Busatto S, Federici S, Ridolfi A, Aikawa M, Bertazzo S, Bergese P, Weinbaum S, Aikawa E.
Published in: Proceedings of the National Academy of Sciences, Issue 118 (14), 2021, Page(s) 1811725118, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1811725118

Nanoparticles and organized lipid assemblies: from interaction to design of hybrid soft devices

Author(s): Marco Mendozza, Lucrezia Caselli, Annalisa Salvatore, Costanza Montis, Debora Berti
Published in: Soft Matter, Issue 15/44, 2019, Page(s) 8951-8970, ISSN 1744-683X
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sm01601e

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Author(s): Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Published in: Nanoscale Horizons, Issue 6/7, 2021, Page(s) 543-550, ISSN 2055-6756
Publisher: The Royal Society of Chemistry 2021
DOI: 10.1039/d1nh00012h

Deterministic Lateral Displacement: Challenges and Perspectives.

Author(s): Hochstetter A, Vernekar R, Austin RH, Becker H, Beech JP, Fedosov DA, Gompper G, Kim SC, Smith JT, Stolovitzky G, Tegenfeldt JO, Wunsch BH, Zeming KK, Krüger T, Inglis DW.
Published in: ACS Nano, Issue 14(9), 2020, Page(s) 10784-10795, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.0c05186

Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces

Author(s): Costanza Montis, Annalisa Salvatore, Francesco Valle, Lucia Paolini, Francesco Carlà, Paolo Bergese, Debora Berti
Published in: Journal of Colloid and Interface Science, Issue 570, 2020, Page(s) 340-349, ISSN 0021-9797
Publisher: Academic Press
DOI: 10.1016/j.jcis.2020.03.014

AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles

Author(s): Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T. Boysen, Francesca Loria, Martijn J. C. van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H. M. Wauben, Debora Berti, Paolo Bergese, Francesco Valle
Published in: Analytical Chemistry, Issue 92/15, 2020, Page(s) 10274-10282, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.9b05716

Fluorescent Labeling of Helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach

Author(s): Anders T. Boysen, Bradley Whitehead, Allan Stensballe, Anna Carnerup, Tommy Nylander, Peter Nejsum
Published in: Biomedicines, Issue 8/7, 2020, Page(s) 213, ISSN 2227-9059
Publisher: Biomedicines
DOI: 10.3390/biomedicines8070213

Regular Industrial Processing of Bovine Milk Impacts the Integrity and Molecular Composition of Extracellular Vesicles

Author(s): Marije Kleinjan, Martijn JC van Herwijnen, Sten FWM Libregts, RJ Joost van Neerven, Anouk L Feitsma, Marca HM Wauben
Published in: The Journal of Nutrition, Issue 151/6, 2021, Page(s) 1416-1425, ISSN 0022-3166
Publisher: American Society for Nutritional Sciences
DOI: 10.1093/jn/nxab031

Cell Sorting Using Electrokinetic Deterministic Lateral Displacement

Author(s): Ho BD, Beech JP, Tegenfeldt JO
Published in: Micromachines, Issue 12(1), 2020, Page(s) 30, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi12010030

Electrokinetic deterministic lateral displacement for fractionation of vesicles and nano-particles

Author(s): Richard J. Gillams, Victor Calero, Raul Fernandez-Mateo and Hywel Morgan
Published in: Lab on a chip, Issue 14730189, 2022, Page(s) 3869-3876, ISSN 1473-0189
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2lc00583b

Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation

Author(s): ANDREA RIDOLFI, LUCREZIA CASELLI, COSTANZA MONTIS, GAETANO MANGIAPIA, DEBORA BERTI, MARCO BRUCALE, FRANCESCO VALLE
Published in: Journal of Microscopy, 2020, ISSN 0022-2720
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/jmi.12910

Small extracellular vesicles from malignant ascites of patients with advanced ovarian cancer provide insights into the dynamics of the extracellular matrix.

Author(s): Bortot B, Apollonio M, Rampazzo E, Valle F, Brucale M, Ridolfi A, Ura B, Addobbati R, Di Lorenzo G, Romano F, Buonomo F, Ripepi C, Ricci G, Biffi S.
Published in: Mol Oncol. 2021 Dec;15(12), Issue Mol Oncol. 2021 Dec;15(12), 2021, Page(s) 3596-3614., ISSN 1878-0261
Publisher: FEBS Press
DOI: 10.1002/1878-0261.13110

Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Author(s): Sara Busatto, George Vilanilam, Taylor Ticer, Wen-Lang Lin, Dennis Dickson, Shane Shapiro, Paolo Bergese, Joy Wolfram
Published in: Cells, Issue 7/12, 2018, Page(s) 273, ISSN 2073-4409
Publisher: https://www.mdpi.com/
DOI: 10.3390/cells7120273

Active Posts in Deterministic Lateral Displacement Devices

Author(s): Jason P. Beech, Kevin Keim, Bao Dang Ho, Carlotta Guiducci, Jonas O. Tegenfeldt
Published in: Advanced Materials Technologies, 2019, Page(s) 1900339, ISSN 2365-709X
Publisher: https://onlinelibrary.wiley.com/
DOI: 10.1002/admt.201900339

The nanostructured secretome

Author(s): S. Busatto, A. Zendrini, A. Radeghieri, L. Paolini, M. Romano, M. Presta, P. Bergese
Published in: Biomaterials Science, Issue 8/1, 2020, Page(s) 39-63, ISSN 2047-4830
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9bm01007f

Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop

Author(s): Ashley E. Russell, Alexandra Sneider, Kenneth W. Witwer, Paolo Bergese, Suvendra N. Bhattacharyya, Alexander Cocks, Emanuele Cocucci, Uta Erdbrügger, Juan M. Falcon-Perez, David W. Freeman, Thomas M. Gallagher, Shuaishuai Hu, Yiyao Huang, Steven M. Jay, Shin-ichi Kano, Gregory Lavieu, Aleksandra Leszczynska, Alicia M. Llorente, Quan Lu, Vasiliki Mahairaki, Dillon C. Muth, Nicole Noren Hooten, Mat
Published in: Journal of Extracellular Vesicles, Issue 8/1, 2019, Page(s) 1684862, ISSN 2001-3078
Publisher: Journal of Extravesicles
DOI: 10.1080/20013078.2019.1684862

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions

Author(s): Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
Published in: Frontiers in Bioengineering and Biotechnology, Issue 7, 2020, ISSN 2296-4185
Publisher: Front. Bioeng. Biotechnol.
DOI: 10.3389/fbioe.2019.00452

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Author(s): Ura B, Biffi S, Monasta L, Arrigoni G, Battisti I, Di Lorenzo G, Romano F, Aloisio M, Celsi F, Addobbati R, Valle F, Rampazzo E, Brucale M, Ridolfi A, Licastro D, Ricci G.
Published in: Cancers, Issue 13(14), 2021, Page(s) 3639, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin

Author(s): Lucia Paolini, Stefania Federici, Giovanni Consoli, Diletta Arceri, Annalisa Radeghieri, Ivano Alessandri, Paolo Bergese
Published in: Journal of Extracellular Vesicles, Issue 9/1, 2020, Page(s) 1741174, ISSN 2001-3078
Publisher: Journal of Extravesicles
DOI: 10.1080/20013078.2020.1741174

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis

Author(s): Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Published in: Cells, Issue 10/3, 2021, Page(s) 544, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells10030544

Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses

Author(s): Marijke I. Zonneveld, Martijn J.C. Herwijnen, Marcela M. Fernandez‐Gutierrez, Alberta Giovanazzi, Anne Marit Groot, Marije Kleinjan, Toni M.M. Capel, Alice J.A.M. Sijts, Leonie S. Taams, Johan Garssen, Esther C. Jong, Michiel Kleerebezem, Esther N.M. Nolte‐’t Hoen, Frank A. Redegeld, Marca H.M. Wauben
Published in: Journal of Extracellular Vesicles, Issue 10/5, 2021, ISSN 2001-3078
Publisher: Taylor and Francis Ltd.
DOI: 10.1002/jev2.12071

Human milk extracellular vesicles preserve bronchial epithelialbarrier integrity and reduce TLR3-induced inflammation in vitro

Author(s): Nikita Karra; Martijn J. C. Van Herwijnen; Marca H. M. Wauben; Emily Jane Swindle; Hywel Morgan
Published in: Journal of Extracellular Biology, Issue 20013078, 2022, Page(s) e56, ISSN 2001-3078
Publisher: Wiley
DOI: 10.1002/jex2.54

Charge-Based Separation of Micro- and Nanoparticles

Author(s): Bao Ho, Jason Beech, Jonas Tegenfeldt
Published in: Micromachines, Issue 11/11, 2020, Page(s) 1014, ISSN 2072-666X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/mi11111014

Shedding light on membrane-templated clustering of gold nanoparticles

Author(s): Costanza Montis, Lucrezia Caselli, Francesco Valle, Andrea Zendrini, Francesco Carlà, Ralf Schweins, Marco Maccarini, Paolo Bergese, Debora Berti
Published in: Journal of Colloid and Interface Science, Issue 573, 2020, Page(s) 204-214, ISSN 0021-9797
Publisher: Academic Press
DOI: 10.1016/j.jcis.2020.03.123