Extracellular vesicles from a natural source for tailor-made nanomaterials

Project Information

VES4US

Grant agreement ID: 801338

DOI 10.3030/801338

Project closed

EC signature date 25 May 2018

Start date 1 September 2018

End date 28 February 2022

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost € 2 946 303,75

EU contribution € 2 946 303,75

Coordinated by

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

Deliverables

Websites, patent fillings, videos etc. (2)

Website and Logo

Ves4Us Logo and Website with intranet section

Optimal culture conditions

Optimised cultivation conditions are determined

Documents, reports (10)

Gender Action Plan

The Gender Action Plan will promote equality within the framework of the project

Quality Management system

A quality plan, compatible to UNI EN ISO 9001:2015 and OECD GLP standards, for the management of procedures

Dissemination and Communication Plan
Training plan

A training plan will describe detailed actions to improve the interdisciplinary methodology and the scientific cross-fertilisation amongst the partners by training staff and students of each participant institution for targeted collaborative experimentation

Cellular response to natural source-derived EVs

The cellular response to EVs will be in depth described

Functionalization strategy report

Description of the state of the art strategy/ies to be applied for micoalgal EV functionalization

First report on outreach activities

Report on outreach activities

Second report on outreach activities

Report on outreach activities

Characterization and purification of functionalized EVs

Microfluidic technology for the characterization and purification of engineered EVs

SOPs for microalgal EVs

SOPs for EVs isolation, storage and transport

Demonstrators, pilots, prototypes (3)

Preclinical studies in rodents

The potential shorttermlongterm systemic toxicity and in vivo stability of microalgaederived EVs will be assessed in rodents

Characteristics of MiPs

MiPs will be characterised and then tested for selectively capturing EVs from the microalgae cultivation media

BubbleMumble game

The science app game BubbleMumble on microalgal EV

Open Research Data Pilot (1)

Data Management Plan

DMP will be based on the 7-point structure as described in the grant agreement

Publications

Peer reviewed articles (35)

Budding and Fission of Membrane Vesicles: A Mini Review

Author(s): Samo Penič, Luka Mesarec, Miha Fošnarič, Lucyna Mrówczyńska, Henry Hägerstrand, Veronika Kralj-Iglič, Aleš Iglič
Published in: Frontiers in Physics, Issue 8, 2020, ISSN 2296-424X
Publisher: Frontiers in Physics
DOI: 10.3389/fphy.2020.00342

Enrichment of plasma in platelets and extracellular vesicles by the counterflow to erythrocyte settling

Author(s): Božič, Darja, Vozel, Domen, Hočevar, Matej, Jeran, Marko, Jan, Zala, Pajnič, Manca, Pađen, Ljubiša, Iglič, Aleš, Battelino, Saba, Kralj-Iglič, Veronika
Published in: Platelets, 2021, ISSN 0953-7104
Publisher: Taylor & Francis
DOI: 10.1080/09537104.2021.1961716

Correlation between adhesion strength and phase behaviour in solid-supported lipid membranes

Author(s): N. Bibissidis, K. Betlem, G. Cordoyiannis, F. Prista-von Bonhorst, J. Goole, J. Raval, M. Daniel, W. Góźdź, A. Iglič, P. Losada-Pérez
Published in: Journal of Molecular Liquids, Issue 320, 2020, Page(s) 114492, ISSN 0167-7322
Publisher: Elsevier BV
DOI: 10.1016/j.molliq.2020.114492

Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate Amphidinium carterae (Subclade III)

Author(s): Maria Elena Barone, Elliot Murphy, Rachel Parkes, Gerard T. A. Fleming, Floriana Campanile, Olivier P. Thomas, Nicolas Touzet
Published in: International Journal of Molecular Sciences, Issue 22/22, 2021, Page(s) 12196, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222212196

Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds

Author(s): Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, El
Published in: Biomaterials Science, Issue 9/8, 2021, Page(s) 2917-2930, ISSN 2047-4830
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0bm01696a

Autologous Platelet- and Extracellular Vesicle-Rich Plasma Is an Effective Treatment Modality for Chronic Postoperative Temporal Bone Cavity Inflammation: Randomized Controlled Clinical Trial

Author(s): Domen Vozel, Darja Božič, Marko Jeran, Zala Jan,Manca Pajnič, Ljubiša Pađen,Nejc Steiner, Veronika Kralj-Iglič, and Saba Battelino
Published in: Front Bioeng Biotechnoly, 2021, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2021.677541

A failure mode and effect analysis (FMEA)-based approach for risk assessment of scientific processes in non-regulated research laboratories

Author(s): A. Mascia, A. M. Cirafici, A. Bongiovanni, G. Colotti, G. Lacerra, M. Di Carlo, F. A. Digilio, G. L. Liguori, A. Lanati, A. Kisslinger
Published in: Accreditation and Quality Assurance, 2020, ISSN 0949-1775
Publisher: Springer Verlag
DOI: 10.1007/s00769-020-01441-9

Comparative Response of Marine Microalgae to H2O2-Induced Oxidative Stress

Author(s): Maria Elena Barone, Rachel Parkes, Helen Herbert, Adam McDonnell, Thomas Conlon, Anita Aranyos, David Fierli, Gerard T. A. Fleming, Nicolas Touzet
Published in: Applied Biochemistry and Biotechnology, Issue 193/12, 2021, Page(s) 4052-4067, ISSN 0273-2289
Publisher: Humana Press, Inc.
DOI: 10.1007/s12010-021-03690-x

Sialylation of host proteins as targetable risk factor for COVID‐19 susceptibility and spreading: A hypothesis

Author(s): Antonella Bongiovanni, Antonella Cusimano, Ida Annunziata, Alessandra d’Azzo
Published in: FASEB BioAdvances, Issue 3/3, 2021, Page(s) 192-197, ISSN 2573-9832
Publisher: PublishMed
DOI: 10.1096/fba.2020-00073

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

Author(s): Giulia Di Prima, Fabio Librizzi, Rita Carrotta
Published in: Membranes, Issue 10/9, 2020, Page(s) 222, ISSN 2077-0375
Publisher: Molecular Diversity Preservation International
DOI: 10.3390/membranes10090222

Subcellular Localization of uc.8+ as a Prognostic Biomarker in Bladder Cancer Tissue

Author(s): Sara Terreri, Sara Mancinelli, Matteo Ferro, Maria Concetta Vitale, Sisto Perdonà, Luigi Castaldo, Vincenzo Gigantino, Vincenzo Mercadante, Rossella De Cecio, Gabriella Aquino, Marco Montella, Claudia Angelini, Eugenio Del Prete, Marianna Aprile, Angelo Ciaramella, Giovanna L. Liguori, Valerio Costa, George A. Calin, Evelina La Civita, Daniela Terracciano, Ferdinando Febbraio, and Amelia Cimmino
Published in: Cancers, 2021, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13040681

Curvature Potential Unveiled Topological Defect Attractors

Author(s): Mesarec, Luka, Iglič, Aleš, Kralj-Iglič, Veronika, Góźdź, Wojciech, Virga, Epifanio G., Kralj, Samo
Published in: Crystals, 2021, ISSN 2073-4352
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cryst11050539

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Author(s): Giorgia Adamo, David Fierli, Daniele P. Romancino, Sabrina Picciotto, Maria E. Barone, Anita Aranyos, Darja Božič, Svenja Morsbach, Samuele Raccosta, Christopher Stanly, Carolina Paganini, Meiyu Gai, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Loredana Randazzo, Rachel Parkes, Umberto Capasso Palmiero, Estella Rao, Angela Paterna, Pamela Santonicola, Ales
Published in: Journal of Extracellular Vesicles, Issue 10/6, 2021, ISSN 2001-3078
Publisher: ISEV
DOI: 10.1002/jev2.12081

The Transcription Regulator Patz1 Is Essential for Neural Stem Cell Maintenance and Proliferation

Author(s): Sara Mancinelli, Michela Vitiello, Maria Donnini, Francesca Mantile, Giuseppe Palma, Antonio Luciano, Claudio Arra, Laura Cerchia, Giovanna Lucia Liguori and Monica Fedele
Published in: Frontiers in Cell and Developmental Biology, 2021, ISSN 2296-634X
Publisher: Original Research
DOI: 10.3389/fcell.2021.657149

On the Role of Curved Membrane Nanodomains and Passive and Active Skeleton Forces in the Determination of Cell Shape and Membrane Budding

Author(s): Luka Mesarec, Mitja Drab, Samo Penič, Veronika Kralj-Iglič, Aleš Iglič
Published in: International Journal of Molecular Sciences, Issue 22/5, 2021, Page(s) 2348, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22052348

Concentration of extracellular vesicles isolated from blood relative to the clinical pathological status of dogs with mast cell tumours

Author(s): Metka Šimundić, Tanja Švara, Roman Štukelj, Judita L Krek, Mitja Gombač, Veronika Kralj-Iglič, Nataša Tozon
Published in: MEDLINE, 2021, ISSN 1476-5829
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/vco.12489

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Author(s): Angela Paterna, Estella Rao, Giorgia Adamo, Samuele Raccosta, Sabrina Picciotto, Daniele Romancino, Rosina Noto, Nicolas Touzet, Antonella Bongiovanni and Mauro Manno
Published in: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Publisher: Frontiers
DOI: 10.3389/fbioe.2022.836747

Species-specific responses in pigments and fatty acids of five freshwater chlorophytes exposed to varying cultivation conditions

Author(s): Rachel Parkes, Maria Elena Barone, Anita Aranyos, David Fierli, Henry Koehler, Eoin Gillespie, Nicolas Touzet
Published in: Process Biochemistry, Issue 112, 2022, Page(s) 35-44, ISSN 1359-5113
Publisher: Elsevier Applied Science
DOI: 10.1016/j.procbio.2021.11.018

Green kiwifruit extracts protect motor neurons from death in a spinal muscular atrophy model in Caenorhabditis elegans

Author(s): Nadia Mazzarella, Ivana Giangrieco, Serena Visone, Pamela Santonicola, Jannis Achenbach, Giuseppina Zampi, Maurizio Tamburrini, Elia Di Schiavi, Maria Antonietta Ciardiello
Published in: Food Science & Nutrition, Issue 7/7, 2019, Page(s) 2327-2335, ISSN 2048-7177
Publisher: Wiley
DOI: 10.1002/fsn3.1078

Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Author(s): Božič, Sitar, Junkar, Štukelj, Pajnič, Žagar, Kralj-Iglič, Kogej
Published in: Cells, Issue 8/9, 2019, Page(s) 1046, ISSN 2073-4409
Publisher: Department of Chemistry and Biochemistry, University of Ljubljana
DOI: 10.3390/cells8091046

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author(s): Clotilde Théry, Kenneth W Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon D Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, Tanina Arab, Fabienne Archer, Georgia K Atkin-Smith, D Craig Ayre, Jean-Marie Bach, Daniel Bachurski, Hossein Baharvand, Leonora Balaj, Shawn Baldacchino, Natalie N Bauer, Amy A Baxter, Mary Bebawy, Carla Beckham, Apolonija Bedina Zavec, Abderrahim Benmoussa, Anna
Published in: Journal of Extracellular Vesicles, Issue 7/1, 2018, Page(s) 1535750, ISSN 2001-3078
Publisher: UK Limited
DOI: 10.1080/20013078.2018.1535750

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Author(s): Carolina Paganini, Umberto Capasso Palmiero, Gabriella Pocsfalvi, Nicolas Touzet, Antonella Bongiovanni, Paolo Arosio
Published in: Biotechnology Journal, 2019, Page(s) 1800528, ISSN 1860-6768
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/biot.201800528

Inception Mechanisms of Tunneling Nanotubes

Author(s): Mitja Drab, David Stopar, Veronika Kralj-Iglič, Aleš Iglič
Published in: Cells, Issue 8/6, 2019, Page(s) 626, ISSN 2073-4409
Publisher: Univerza v Ljubljani
DOI: 10.3390/cells8060626

Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles

Author(s): Christopher Stanly, Maneea Moubarak, Immacolata Fiume, Lilla Turiák, Gabriella Pocsfalvi
Published in: International Journal of Molecular Sciences, Issue 20/24, 2019, Page(s) 6205, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20246205

A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly

Author(s): Meiyu Gai, Johanna Simon, Ingo Lieberwirth, Volker Mailänder, Svenja Morsbach, Katharina Landfester
Published in: Polymer Chemistry, 2020, ISSN 1759-9954
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9py01136f

Vaccination with (1–11)E2 in alum efficiently induces an antibody response to β-amyloid without affecting brain β-amyloid load and microglia activation in 3xTg mice

Author(s): Francesca Mantile, Angelo Capasso, Nadia Villacampa, Maria Donnini, Giovanna L. Liguori, Gabriela Constantin, Piergiuseppe De Berardinis, Michael T. Heneka, Antonella Prisco
Published in: Aging Clinical and Experimental Research, 2019, ISSN 1720-8319
Publisher: Springer International Publishing
DOI: 10.1007/s40520-019-01414-0

Normal red blood cells’ shape stabilized by membrane’s in-plane ordering

Author(s): L. Mesarec, W. Góźdź, A. Iglič, V. Kralj-Iglič, E. G. Virga, S. Kralj
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-56128-0

Role of PA2G4P4 pseudogene in bladder cancer tumorigenesis

Author(s): Laura Pisapia, Sara Terreri, Pasquale Barba, Marianna Mastroianni, Maria Donnini, Vincenzo Mercadante, Alessandro Palmieri, Paolo Verze, Vincenzo Mirone, Vincenzo Altieri, Gianluigi Califano, Giovanna Lucia Liguori, Maria Strazzullo, Amelia Cimmino, Giovanna Del Pozzo
Published in: Biology, Issue 9/4, 2020, Page(s) 66, ISSN 2079-7737
Publisher: Biology
DOI: 10.3390/biology9040066

Minimizing isotropic and deviatoric membrane energy – An unifying formation mechanism of different cellular membrane nanovesicle types

Author(s): Veronika Kralj-Iglič, Gabriella Pocsfalvi, Luka Mesarec, Vid Šuštar, Henry Hägerstrand, Aleš Iglič
Published in: PLOS ONE, Issue 15/12, 2020, Page(s) e0244796, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0244796

Quality Management Tools on the Stage: Old but New Allies for Rigor and Standardization of Extracellular Vesicle Studies

Author(s): Giovanna L. Liguori, Annamaria Kisslinger
Published in: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Publisher: Original Research
DOI: 10.3389/fbioe.2022.826252

Design of Transfections (DoT): implementation of Design of Experiments for cell transfection fine-tuning.

Author(s): Sara Mancinelli; Andrea Turcato; Annamaria Kisslinger; Antonella Bongiovanni; Valeria Zazzu; Antonella Lanati; Giovanna L. Liguori
Published in: Biotechnology and Bioengineering, Issue 1, 2021, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27918

Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon

Author(s): Zala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič
Published in: International Journal of Nanomedicine, Issue Volume 16, 2021, Page(s) 443-456, ISSN 1178-2013
Publisher: Laboratory of Clinical Biophysics
DOI: 10.2147/ijn.s282200

Mechanical and Electrical Interaction of Biological Membranes with Nanoparticles and Nanostructured Surfaces

Author(s): Jeel Raval, Ekaterina Gongadze, Metka Benčina, ,Ita Junkar, Niharika Rawat, Luka Mesarec,Veronika Kralj-Iglič,Wojciech Góźdź and Aleš Iglič
Published in: Membranes, 2021, ISSN 2077-0375
Publisher: Molecular Diversity Preservation International
DOI: 10.3390/membranes11070533

The effect of lipid phase on liposome stability upon exposure to the mechanical stress

Author(s): Joanna Doskocz, Paulina Dałek, Aleksander Foryś, Barbara Trzebicka, Magdalena Przybyło, Luka Mesarec, Aleš Iglič, Marek Langner
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, Issue 1862/9, 2020, Page(s) 183361, ISSN 0005-2736
Publisher: Elsevier BV
DOI: 10.1016/j.bbamem.2020.183361

Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence‐Based Microfluidic Diffusion Sizing

Author(s): Carolina Paganini, Britta Hettich, Marie R.G. Kopp, Adam Eördögh, Umberto Capasso Palmiero, Giorgia Adamo, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni, Pablo Rivera‐Fuentes, Jean‐Christophe Leroux, Paolo Arosio
Published in: Advanced Healthcare Materials, 2021, Page(s) 2100021, ISSN 2192-2640
Publisher: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202100021

Intellectual Property Rights

Patent (2)

EXTRACELLULAR VESICLES FROM MICROALGAE

Application/Publication number: 20 20086622
Date: 2020-12-17
Applicant(s): INSTITUTE OF TECHNOLOGY SLIGO - ITS

EXTRACELLULAR VESICLES FROM MICROALGAE

Application/Publication number: 20 20086622
Date: 2020-12-17