Building the product pipeline for commercial demonstration of Plant Molecular Factories

Project Information

Pharma-Factory

Grant agreement ID: 774078

DOI 10.3030/774078

Project closed

EC signature date 6 October 2017

Start date 1 November 2017

End date 31 August 2022

Funded under

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Total cost € 9 254 335,00

EU contribution € 8 286 008,63

Coordinated by

ST GEORGE'S HOSPITAL MEDICAL SCHOOL
United Kingdom

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (1)

GMP guidance

Guidance on GMP compliance in Plant Molecular Farming

Demonstrators, pilots, prototypes (2)

Marketed kit

Recombinant protein kit placed on market

Diagnostic kit for sero-negative RA on the market

Other (1)

Public exhibits for PMF

3 public exhibits

Websites, patent fillings, videos etc. (2)

Project website

Public and project website

Social media and PMF website established

Social media strategies and setup of an overarching public website for Plant Molecular Farming (not to be confused with the project website).

Publications

Peer reviewed articles (34)

Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process

Author(s): Noemi Gutierrez-Valdes, Suvi T. Häkkinen, Camille Lemasson, Jonas de Groot, Jean-Pierre Ele-Ekouna, Marina Guillet, Florian Cardon, Anneli Ritala
Published in: Biotechnology and Bioengineering, Issue 00063592, 2022, Page(s) 2831-2841, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28178

Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum

Author(s): Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, Carlos Afonso, Patrice Lerouge, Muriel Bardor
Published in: Carbohydrate Polymers, Issue 259, 2021, Page(s) 117660, ISSN 0144-8617
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2021.117660

A simplified techno‐economic model for the molecular pharming of antibodies

Author(s): Pere Mir‐Artigues, Richard M. Twyman, Derry Alvarez, Pedro Cerda Bennasser, Merce Balcells, Paul Christou, Teresa Capell
Published in: Biotechnology and Bioengineering, Issue 116/10, 2019, Page(s) 2526-2539, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27093

N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

Author(s): Elodie Mathieu-Rivet, Narimane Mati-Baouche, Marie-Laure Walet-Balieu, Patrice Lerouge, Muriel Bardor
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.609993

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Author(s): Noemi Gutierrez-Valdes, Suvi T. Häkkinen, Camille Lemasson, Marina Guillet, Kirsi-Marja Oksman-Caldentey, Anneli Ritala, Florian Cardon
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.00033

The concept of an agroinfiltration kit for recombinant protein production for educational and commercial use—A journey through a forest of regulatory and legal implications

Author(s): Holger Spiegel, Greta Nölke, Harry Thangaraj and Stefan Schillberg
Published in: Frontiers in Bioengineering and Biotechnology, Issue 22964185, 2022, ISSN 2296-4185
Publisher: Frontiers SA
DOI: 10.3389/fbioe.2022.926239

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Author(s): Borja Diego-Martin, Beatriz González, Marta Vazquez-Vilar, Sara Selma, Rubén Mateos-Fernández, Silvia Gianoglio, Asun Fernández-del-Carmen, Diego Orzáez
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.612781

Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics

Author(s): Hadrien Peyret, Elisabetta Groppelli, David Clark, Nicholas Eckersley, Tim Planche, Julian Ma, George P. Lomonossoff
Published in: Journal of Virological Methods, Issue 01660934, 2022, ISSN 0166-0934
Publisher: Elsevier BV
DOI: 10.1016/j.jviromet.2021.114372

Plant Molecular Farming as a Strategy Against COVID-19 - The Italian Perspective

Author(s): Chiara Lico; Luca Santi; Selene Baschieri; Emanuela Noris; Carla Marusic; Marcello Donini; Emanuela Pedrazzini; Giovanni Maga; Rosella Franconi; Paola Di Bonito; Linda Avesani
Published in: Frontiers in plant science 11 (2020). doi:10.3389/fpls.2020.609910, Issue 2, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.609910

Physicochemical characterization of the recombinant lectin scytovirin and microbicidal activity of the SD1 domain produced in rice against HIV‑1

Author(s): Victoria Armario-Najera, Amaya Blanco-Perera, Shilpa R. Shenoy, Yi Sun, Silvia Marfil, Jordana Muñoz-Basagoiti, Daniel Perez-Zsolt, Julià Blanco, Nuria Izquierdo-Useros, Teresa Capell, Barry R. O’Keefe & Paul Christou
Published in: Plant Cell Reports, 2022, Page(s) 1013-1023, ISSN 0721-7714
Publisher: Springer Verlag
DOI: 10.1007/s00299-022-02834-5

Investigation of a monoclonal antibody against enterotoxigenic Escherichia coli, expressed as secretory IgA1 and IgA2 in plants

Author(s): Audrey Y-H Teh,Lisa Cavacini,Yue Hu,Ozan S. Kumru,Jian Xiong,David T. BolickORCID Icon,Sangeeta B. Joshi,Clemens Grünwald-Gruber,Friedrich Altmann,Mark Klempner,Richard L. Guerrant,David B. Volkin, Yang Wang and Julian K-C. Ma
Published in: Gut Microbes, Issue volume 13 issue 1, 2021, Page(s) 1-14, ISSN 1949-0984
Publisher: Taylor Francis online
DOI: 10.1080/19490976.2020.1859813

A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants

Author(s): Eva C. Thuenemann, Matthew J. Byrne, Hadrien Peyret, Keith Saunders, Roger Castells-Graells ,Inmaculada Ferriol, Mattia Santoni, John F. C. Steele, Neil A. Ranson, Linda Avesani, Juan Jose Lopez-Moya and George P. Lomonossoff
Published in: Viruses, 2021, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13050885

Plant Virus Nanoparticles for Vaccine Applications

Author(s): Mattia Santoni, Roberta Zampieri, Linda Avesani
Published in: Current Protein & Peptide Science, Issue 21/4, 2020, Page(s) 344-356, ISSN 1389-2037
Publisher: Bentham Science Publishers
DOI: 10.2174/1389203721666200212100255

Multiple gene expression in plants using MIDAS-P, a versatile type II restriction-based modular expression vector.

Author(s): Elizabeth C. Pinneh, Craig J. van Dolleweerd, Kathrin Göritzer, Pascal M. W. Drake, Julian K-C. Ma, Audrey Y-H. Teh
Published in: Biotechnology and Bioengineering, 2022, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28073

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Author(s): Norma Aliaga-Franco1, Cunjin Zhang2, Silvia Presa1, Anjil K. Srivastava2, Antonio Granell1, David Alabadí1, Ari Sadanandom2, Miguel A. Blázquez1 and Eugenio G. Minguet1*
Published in: Fronteirs in Plant Science, 2019, ISSN 1664-462x
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2019.01150

Contributions of the international plant science community to the fight against human infectious diseases – part 1: epidemic and pandemic diseases

Author(s): Lobato, Maria; Huang, Xin; Alvarez, Derry; He, Wenshu; Baysal, Can; Zhu, Changfu; Armario-Nájera, Victoria; Blanco Perera, Amaya; Cerda, Pedro; Saba-Mayoral, Andrea; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes F.; Cunha, Nicolau B. da; Daniell, Henry; Faller, Roland; Folgado, André; Gowtham, Iyapp
Published in: Plant Biotechnology Journal, 2021, Page(s) 1901-1920, ISSN 1467-7644
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13657

Potential Applications of Plant Biotechnology against SARS-CoV-2

Author(s): Teresa Capell, Richard M. Twyman, Victoria Armario-Najera, Julian K.-C. Ma, Stefan Schillberg, Paul Christou
Published in: Trends in Plant Science, Issue 25/7, 2020, Page(s) 635-643, ISSN 1360-1385
Publisher: Elsevier BV
DOI: 10.1016/j.tplants.2020.04.009

Investigation of the N-Glycosylation of the SARS-CoV-2 S Protein Contained in VLPs Produced in Nicotiana benthamiana

Author(s): Juliette Balieu, Jae-Wan Jung, Philippe Chan, George P. Lomonossoff, Patrice Lerouge, and Muriel Bardor
Published in: Molecules, Issue 27, 2022, Page(s) 5119, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules27165119

Comparative Proteomic Analysis of the Diatom Phaeodactylum tricornutum Reveals New Insights Into Intra- and Extra-Cellular Protein Contents of Its Oval, Fusiform, and Triradiate Morphotypes

Author(s): Coralie Chuberre, Philippe Chan, Marie-Laure Walet-Balieu, François Thiébert, Carole Burel, Julie Hardouin, Bruno Gügi and Muriel Bardor
Published in: Frontiers in Plant Science, 2022, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2022.673113

Plant-based expression and characterization of SARS-CoV-2 virus-like particles presenting a native spike protein

Author(s): Jae-Wan Jung, Gergana Zahmanova, Ivan Minkov, George P. Lomonossoff
Published in: Plant Biotechnology Journal, Issue 20, 2022, ISSN 1467-7644
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13813

Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes

Author(s): Charlotte Toustou, Marie-Laure Walet-Balieu, Marie-Christine Kiefer-Meyer, Marine Houdou, Patrice Lerouge, François Foulquier, Muriel Bardor
Published in: Biological Reviews, Issue 14647931, 2021, Page(s) 732-748, ISSN 1464-7931
Publisher: Cambridge University Press
DOI: 10.1111/brv.12820

Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products.

Author(s): Jonathan Menary; Matthew Hobbs; Sara Mesquita de Albuquerque; Agata Pacho; Pascal M. W. Drake; Alison Prendiville; Julian K-C. Ma; Sebastian S. Fuller
Published in: PLos One, Issue 2, 2020, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.31235/osf.io/gwve2

The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization

Author(s): Keith Saunders, Eva C. Thuenemann, Sachin N. Shah, Hadrien Peyret, Ruth Kristianingsih, Sergio G. Lopez, Jake Richardson and George P. Lomonossoff
Published in: Frontiers in Bioengineering and Biotechnology, Issue 22964185, 2022, ISSN 2296-4185
Publisher: Frontiers SA
DOI: 10.3389/fbioe.2022.877361

Unexpected synergistic HIV neutralization by a triple microbicide produced in rice endosperm

Author(s): Evangelia Vamvaka, Gemma Farré, Luis M. Molinos-Albert, Abbey Evans, Anna Canela-Xandri, Richard M. Twyman, Jorge Carrillo, Raziel A. Ordóñez, Robin J. Shattock, Barry R. O’Keefe, Bonaventura Clotet, Julian Blanco, Gurdev S. Khush, Paul Christou, Teresa Capell
Published in: Proceedings of the National Academy of Sciences, Issue 115/33, 2018, Page(s) E7854-E7862, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1806022115

Optimizing product quality in molecular farming

Author(s): Andreas Schiermeyer
Published in: Current Opinion in Biotechnology, Issue 61, 2020, Page(s) 15-20, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2019.08.012

Engineering the interactions between a plant‐produced HIV antibody and human Fc receptors

Author(s): Szymon Stelter, Mathew J. Paul, Audrey Y.‐H. Teh, Melanie Grandits, Friedrich Altmann, Jessica Vanier, Muriel Bardor, Alexandra Castilho, Rachel Louise Allen, Julian K‐C. Ma
Published in: Plant Biotechnology Journal, Issue 18/2, 2019, Page(s) 402-414, ISSN 1467-7644
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13207

Characterisation of a highly potent and near pan-neutralising anti-HIV monoclonal antibody expressed in tobacco plants

Author(s): Catherine M. Moore, Melanie Grandits, Clemens Grünwald-Gruber, Friedrich Altmann, Maria Kotouckova, Audrey Y.-H. Teh & Julian K.-C. Ma
Published in: Retrovirology, Issue 18, 2021, ISSN 1742-4690
Publisher: BioMed Central
DOI: 10.21203/rs.3.rs-104508/v1

Molecular Pharming for low and middle income countries

Author(s): Sheeba Murad, Sebastian Fuller, Jonathan Menary, Cathy Moore, Elizabeth Pinneh, Tim Szeto, Inga Hitzeroth, Marcos Freire, Suthira Taychakhoonavudh, Waranyoo Phoolcharoen, Julian K-C Ma
Published in: Current Opinion in Biotechnology, Issue 61, 2020, Page(s) 53-59, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2019.10.005

Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain.

Author(s): Galas, Ludovic; Burel, Carole; Schapman, Damien; Ropitaux, Marc; Bernard, Sophie; Bénard, Magalie; Bardor, Muriel
Published in: Frontiers in Plant Science, Issue 2, 2021, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2021.638181

Contributions of the international plant science community to the fight against infectious diseases in humans-part 2: Affordable drugs in edible plants for endemic and re-emerging diseases.

Author(s): He, Wenshu; Baysal, Can; Lobato, Maria; Huang, Xin; Alvarez, Derry; Zhu, Changfu; Armario-Nájera, Victoria; Blanco Perera, Amaya; Cerda, Pedro; Saba-Mayoral, Andrea; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes F.; Cunha, Nicolau B. da; Daniell, Henry; Faller, Roland; Folgado, André; Gowtham, Iyapp
Published in: Plant Biotechnology Journal, 2021, Page(s) 1921-1936, ISSN 1467-7644
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13658

Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges

Author(s): Stefan Schillberg, Ricarda Finnern
Published in: Journal of Plant Physiology, Issue 258-259, 2021, Page(s) 153359, ISSN 0176-1617
Publisher: Elsevier BV
DOI: 10.1016/j.jplph.2020.153359

Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay

Author(s): Jan Pietschmann1*†, Nadja Voepel1†, Leonie Voß1, Stefan Rasche1, Max Schubert1, Michael Kleines2, Hans-Joachim Krause3, Tamlyn M. Shaw4, Holger Spiegel1‡ and Florian Schroeper1‡
Published in: Frontiers in Microbiology, 2021, ISSN 1664-302x
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.643275

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins

Author(s): Stefan Schillberg, Nicole Raven, Holger Spiegel, Stefan Rasche, Matthias Buntru
Published in: Frontiers in Plant Science, Issue 10, 2019, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2019.00720

Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression

Author(s): Holger Spiegel, Stefan Schillberg & Greta Nölke
Published in: Methods in Molecular Biology - Recombinant Proteins in Plants, Issue 10643745, 2022, Page(s) 89-102, ISSN 1064-3745
Publisher: Humana Press, Inc.
DOI: 10.1007/978-1-0716-2241-4_6

Conference proceedings (2)

Project proximities: A meta review of how design addresses distance in complex collaborations

Author(s): Rosie Hornbuckle
Published in: Lockton, D., Lenzi, S., Hekkert, P., Oak, A., Sádaba, J., Lloyd, P. (eds.), DRS2022: Bilbao, 25 June - 3 July, Bilbao, Spain, 2022
Publisher: Design Research Society
DOI: 10.21606/drs.2022.677

Beyond Science Communication: a service design approach to building mutual stakeholder understanding in the development of novel biotechnologies.

Author(s): Hornbuckle, Rosie and Grimaldi, Silvia and Prendiville, Alison
Published in: Proceedings of the 6th conference on Design4Health. 2020 Lab4Living, Sheffield Hallam University, 2020, Page(s) 230-238, ISBN 978-1-8381117-0-0
Publisher: Sheffield Hallam University
DOI: 10.5281/zenodo.7188867

Non-peer reviewed articles (1)

Pilot production of SARS-CoV-2 related proteins in plants: a proof of concept for rapid repurposing of indoors farms into biomanufacturing facilities

Author(s): Diego-Martin, Borja; González Beatriz; Vázquez Vilar, Marta; Selma, Sara; Mateos-Fernández, Rubén; Gianoglio, Silvia; Fernández-del-Carmen, Asun; Orzáez, Diego
Published in: BioRxiv, 2020, ISSN 2692-8205
Publisher: cold spring harbor laboratory
DOI: 10.1101/2020.10.13.331306

Intellectual Property Rights

Utility model (2)

Product composed of a large-scale bioreactor specifically developed for hairy root growth

Application/Publication number: FR 22 305 436.2
Date: 2022-04-11
Applicant(s): SAMABRIVA

Product composed of a large-scale bioreactor specifically developed for hairy root growth

Application/Publication number: FR 22 305 436.2
Date: 2022-04-11

Patent (3)

METHODS FOR THE IMPROVED RECOVERY OF SECRETED COMPOUNDS IN A HAIRY ROOTS-BASED EXPRESSION SYSTEM

Application/Publication number: 20 21083070
Date: 2021-11-25
Applicant(s): SAMABRIVA

METHOD FOR ESTIMATING THE BIOMASS OF HAIRY ROOTS IN A MULTI-PHASIC CULTURE

Application/Publication number: 20 22067056
Date: 2022-06-22

METHOD FOR ESTIMATING THE BIOMASS OF HAIRY ROOTS IN A MULTI-PHASIC CULTURE

Application/Publication number: 20 22067056
Date: 2022-06-22
Applicant(s): SAMABRIVA