Developing Multipurpose Nicotiana Crops for Molecular Farming using New Plant Breeding Techniques

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Collection of functionally characterized intragenic elements
Report about Public Perception of NPBTs
A transferable universal GT toolbox for Nicotiana and other Solanaceae species
White paper on the assessment of NPBTs for future breeding practice
Communication Plan and Gender Action Plan
Final Communication Report
Initial report on Stakeholder information of NPBTs and Molecular Farming
Collection of editing enzymes with compared efficiencies and predictive model
Final Dissemination Report
Final report on stakeholder opinions and expected impacts of NPBTs
Final Report on Regulation and Biosafety updates
Initial Report on Regulation and Biosafety updates
Dissemination Plan

Development of Apollo web based genome browser for PacBio N. benthamiana genome assembly
Webtool hosting dedicated software and DNA part documentation
Collection of web based and audio-visual communication materials

PacBio sequencing and assembly of N. benthaminana genome

Stable N. benthamiana line expressing intronized Cas9

Publicaciones

PYL1- and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue

Autores: Pizzio GA, Mayordomo C, Lozano-Juste J, Garcia-Carpintero V, Vazquez-Vilar M, Nebauer SG, Kaminski KP, Ivanov NV, Estevez JC, Rivera-Moreno M, Albert A, Orzaez D, Rodriguez PL.
Publicado en: Cells, 2022, ISSN 2073-4409
Editor: MDPI AG
DOI: 10.3390/cells11050795

Engineering CRISPR/ Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing

Autores: Patrick Schindele, Holger Puchta
Publicado en: Plant Biotechnology Journal, Edición 18/5, 2020, Página(s) 1118-1120, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13275

Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/ Lb Cas12a

Autores: Laura Merker, Patrick Schindele, Teng‐Kuei Huang, Felix Wolter, Holger Puchta
Publicado en: Plant Biotechnology Journal, Edición 18/12, 2020, Página(s) 2382-2384, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13426

Strong and tunable anti-CRISPR/Cas activities in plants

Autores: Calvache, Camilo; Vazquez-Vilar, Marta; Selma, Sara; Uranga, Mireia; Fernández-del-Carmen, Asun; Daròs, José-Antonio; Orzaez, Diego
Publicado en: Plant Biotechnology Journal, Edición 4, 2022, Página(s) 399-408, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13723

Multiple gene expression in plants using MIDAS-P, a versatile type II restriction-based modular expression vector

Autores: Elizabeth C. Pinneh, Craig J. van Dolleweerd, Kathrin Göritzer, Pascal M. W. Drake, Julian K-C. Ma, Audrey Y-H. Teh
Publicado en: Biotechnology and Bioengineering, 2022, Página(s) 1660 - 1672, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28073

Contributions of the international plant science community to the fight against infectious diseases inhumans—part 2: Affordable drugs in edible plants forendemic and re-emerging diseases

Autores: He, Wenshu; Baysal, Can; Lobato Gómez, María; Huang, Xin; Alvarez, Derry; Zhu, Changfu; Armario-Najera, Victoria; Blanco Perera, Amaya; Cerda Bennasser, Pedro; Saba-Mayoral, Andrea; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes F.; da Cunha, Nicolau B.; Daniell, Henry; Faller, Roland; Folgado, Andr
Publicado en: Plant Biotechnology Journal, Edición 19, 2021, Página(s) 1921-1936, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13658

Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges

Autores: Stefan Schillberg, Ricarda Finnern
Publicado en: Journal of Plant Physiology, Edición 258-259, 2021, Página(s) 153359, ISSN 0176-1617
Editor: Elsevier BV
DOI: 10.1016/j.jplph.2020.153359

Metabolomics should be deployed in the identification and characterization of gene‐edited crops

Autores: Paul D. Fraser, Asaph Aharoni, Robert D. Hall, Sanwen Huang, James J. Giovannoni, Uwe Sonnewald, Alisdair R. Fernie
Publicado en: The Plant Journal, Edición 102/5, 2020, Página(s) 897-902, ISSN 0960-7412
Editor: Blackwell Publishing Inc.
DOI: 10.1111/tpj.14679

Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

Autores: Mireia Uranga, Verónica Aragonés, Sara Selma, Marta Vázquez‐Vilar, Diego Orzáez, José‐Antonio Daròs
Publicado en: The Plant Journal, 2021, Página(s) 555–565, ISSN 0960-7412
Editor: Blackwell Publishing Inc.
DOI: 10.1111/tpj.15164

Targeted genome editing of plants and plant cells for biomanufacturing

Autores: Buyel, Johannes F; Stöger, Eva; Bortesi, Luisa
Publicado en: Transgenic Research, Edición 2, 2021, Página(s) 401–426, ISSN 0962-8819
Editor: Chapman & Hall
DOI: 10.1007/s11248-021-00236-z

Bottlenecks and opportunities for synthetic biology biosafety standards

Autores: Pei, L., Garfinkel, M. & Schmidt, M.
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-29889-y

New Hope for a “Cursed” Crop? Understanding Stakeholder Attitudes to Plant Molecular Farming With Modified Tobacco in Europe

Autores: Jonathan Menary, Mario Amato, Andrés Cid Sanchez, Matthew Hobbs, Agata Pacho, Sebastian S. Fuller
Publicado en: Frontiers in Plant Science, Edición 11, 2020, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.00791

Metabolic effects of agro-infiltration on N. benthamiana accessions

Autores: Drapal. Margit; Enfissi, Eugenia M.A.; Fraser, Paul D.
Publicado en: Transgenic Research, 2021, Página(s) 303-315, ISSN 0962-8819
Editor: Chapman & Hall
DOI: 10.1007/s11248-021-00256-9

A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana

Autores: Garcia-Perez, Elena; Diego-Martin, Borja; Quijano-Rubio, Alfredo; Moreno-Giménez, Elena; Selma, Sara; Orzaez, Diego; Vazquez-Vilar, Marta
Publicado en: BMC Biotechnology, Edición 22 (12), 2022, ISSN 1472-6750
Editor: BioMed Central
DOI: 10.1186/s12896-022-00741-x

Expanding the genome-targeting scope and the site selectivity of high-precision base editors

Autores: Junjie Tan, Fei Zhang, Daniel Karcher, Ralph Bock
Publicado en: Nature Communications, Edición 11/1, 2020, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-14465-z

DNA base editing in nuclear and organellar genomes

Autores: Junjie Tan ,Joachim Forner, Daniel Karcher, Ralph Bock
Publicado en: Trends in Genetics, 2022, ISSN 0168-9525
Editor: Elsevier BV
DOI: 10.1016/j.tig.2022.06.015

Contributions of the international plant sciencecommunity to the fight against human infectious diseases–part 1: epidemic and pandemic diseases

Autores: Lobato Gómez, María; Huang, Xin; Alvarez, Derry; He, Wenshu; Baysal, Can; Zhu, Changfu; Armario-Najera, Victoria; Blanco Perera, Amaya; Cerda Bennasser, Pedro; Saba-Mayoral, Andera; Sobrino-Mengual, Guillermo; Vargheese, Ashwin; Abranches, Rita; Abreu, Isabel Alexandra; Balamurugan, Shanmugaraj; Bock, Ralph; Buyel, Johannes.F.; da Cunha, Nicolau B.; Daniell, Henry; Faller, Roland; Folgado, Andr
Publicado en: Plant Biotechnology Journal, Edición 19, 2021, Página(s) 1901–1920, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13657

Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products

Autores: Jonathan Menary, Matthew Hobbs, Sara Mesquita de Albuquerque, Agata Pacho, Pascal M. W. Drake, Alison Prendiville, Julian K-C. Ma, Sebastian S. Fuller
Publicado en: PLOS ONE, Edición 15/3, 2020, Página(s) e0229952, ISSN 1932-6203
Editor: Public Library of Science
DOI: 10.1371/journal.pone.0229952

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Autores: Borja Diego-Martin, Beatriz González, Marta Vazquez-Vilar, Sara Selma, Rubén Mateos-Fernández, Silvia Gianoglio, Asun Fernández-del-Carmen, Diego Orzáez
Publicado en: Frontiers in Plant Science, Edición 11, 2020, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.612781

ABCC transporters mediate the vacuolar accumulation of crocins in saffron stigmas

Autores: Olivia Costantina Demurtas, Rita Francisco, Gianfranco Diretto, Paola Ferrante, Sarah Frusciante, Marco Pietrella, Giuseppe Aprea, Lorenzo Borghi, Mistianne Feeney, Lorenzo Frigerio, Adriana Coricello, Giosuè Costa, Stefano Alcaro, Enrico Martinoia, Giovanni Giuliano
Publicado en: The Plant Cell, 2019, Página(s) tpc.00193.2019, ISSN 1040-4651
Editor: American Society of Plant Biologists
DOI: 10.1105/tpc.19.00193

Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a

Autores: Teng‐Kuei Huang, Brittney Armstrong, Patrick Schindele, Holger Puchta
Publicado en: Plant Biotechnology Journal, 2021, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13546

The chemotype core collection of genus Nicotiana

Autores: Margit Drapal, Eugenia M. A. Enfissi, Paul D. Fraser
Publicado en: The Plant Journal, 2022, Página(s) 1516-1528, ISSN 0960-7412
Editor: Blackwell Publishing Inc.
DOI: 10.1111/tpj.15745

Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9

Autores: Göritzer K, Grandits M, Grünwald-Gruber C, Figl R, Mercx S, Navarre C, Ma JK-C and Teh AY-H
Publicado en: Frontiers in Plant Science, 2022, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2022.1003065

Heterologous expression of Bixa orellana cleavage dioxygenase 4–3 drives crocin but not bixin biosynthesis

Autores: Frusciante, Sarah; Demurtas, Olivia; Sulli, Maria; Mini, Paola; Aprea, Giuseppe; Diretto, Gianfranco; Karcher, Daniel; Bock, Ralph; Giuliano, Giovanni
Publicado en: Plant Physiology, 2021, ISSN 0032-0889
Editor: American Society of Plant Biologists
DOI: 10.1093/plphys/kiab583

Assessment of Cas12a‐mediated gene editing efficiency in plants

Autores: Joan Miquel Bernabé‐Orts, Iván Casas‐Rodrigo, Eugenio G. Minguet, Viola Landolfi, Victor Garcia‐Carpintero, Silvia Gianoglio, Marta Vázquez‐Vilar, Antonio Granell, Diego Orzaez
Publicado en: Plant Biotechnology Journal, 2019, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13113

Novel biotechnological approaches to produce biological compounds: challenges and opportunities for science communication

Autores: Lei Pei, Markus Schmidt
Publicado en: Current Opinion in Biotechnology, Edición 56, 2019, Página(s) 43-47, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2018.08.012

Activity‐based proteomics reveals nine target proteases for the recombinant protein‐stabilizing inhibitor Sl CYS 8 in Nicotiana benthamiana

Autores: Philippe V. Jutras, Friederike Grosse‐Holz, Farnusch Kaschani, Markus Kaiser, Dominique Michaud, Renier A.L. Hoorn
Publicado en: Plant Biotechnology Journal, Edición 17, 2019, Página(s) 1670–1678, ISSN 1467-7644
Editor: Blackwell Publishing Inc.
DOI: 10.1111/pbi.13092

Engineering of high-precision base editors for site-specific single nucleotide replacement

Autores: Junjie Tan; Fei Zhang; Daniel Karcher; Ralph Bock
Publicado en: Nature Communications, Edición 1, 2019, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.5281/zenodo.2586572

High-level expression of the HIV entry inhibitor griffithsin from the plastid genome and retention of biological activity in dried tobacco leaves

Autores: Matthijs Hoelscher, Nadine Tiller, Audrey Y.-H. Teh, Guo-Zhang Wu, Julian K-C. Ma, Ralph Bock
Publicado en: Plant Molecular Biology, Edición 97/4-5, 2018, Página(s) 357-370, ISSN 0167-4412
Editor: Kluwer Academic Publishers
DOI: 10.1007/s11103-018-0744-7

Construction of a fusion enzyme for astaxanthin formation and its characterisation in microbial and plant hosts: A new tool for engineering ketocarotenoids

Autores: Marilise Nogueira, Eugenia M.A. Enfissi, Ralf Welsch, Peter Beyer, Matias D. Zurbriggen, Paul D. Fraser
Publicado en: Metabolic Engineering, Edición 52, 2019, Página(s) 243-252, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2018.12.006

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins

Autores: Stefan Schillberg, Nicole Raven, Holger Spiegel, Stefan Rasche, Matthias Buntru
Publicado en: Frontiers in Plant Science, Edición 10, 2019, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2019.00720

CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors

Autores: Uranga, Mireia; Vazquez-Vilar, Marta; Orzáez, Diego; Darós, José-Antonio
Publicado en: The CRISPR Journal, 2021, Página(s) 761-769, ISSN 2573-1599
Editor: Mary Ann Liebert, Inc., publishers
DOI: 10.1089/crispr.2021.0049

Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics

Autores: Peyret, Hadrien; Groppelli, Elisabetta; Clark, David; Eckersley, Nicholas; Planche, Tim; Ma, Julian; Lomonossoff, George P.
Publicado en: Journal of Virological Methods, 2021, ISSN 0166-0934
Editor: Elsevier BV
DOI: 10.1016/j.jviromet.2021.114372

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Autores: Norma Aliaga-Franco, Cunjin Zhang, Silvia Presa, Anjil K. Srivastava, Antonio Granell, David Alabadí, Ari Sadanandom, Miguel A. Blázquez, Eugenio G. Minguet
Publicado en: Frontiers in Plant Science, Edición 10, 2019, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2019.01150

The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants

Autores: Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabé-Orts JM, Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M, Quintela JC, Fernández-del-Carmen A, Granell A and Orzáez D
Publicado en: Frontiers in Plant Science, 2021, ISSN 1664-462X
Editor: Frontiers Media S. A.
DOI: 10.3389/fpls.2021.689937

Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering

Autores: Huang, Teng-Kuei; Puchta, Holger
Publicado en: Transgenic Research, Edición 3, 2021, Página(s) 529–549, ISSN 0962-8819
Editor: Chapman & Hall
DOI: 10.1007/s11248-021-00238-x

GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants

Autores: Moreno-Giménez, Elena; Selma, Sara; Calvache, Camilo; Orzaez, Diego
Publicado en: ACS Synthetic Biology, Edición 11, 2022, Página(s) 3037−3048, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.2c00238

A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology

Autores: Buddhini Ranawaka, Jiyuan An, Michał T. Lorenc, Hyungtaek Jung, Maria Sulli, Giuseppe Aprea, Sally Roden, Satomi Hayashi, Tal Cooper, Zacharie LeBlanc, Victor Llaca, Diego Orzaez, Aureliano Bombarely, Julia Bally, Christopher Winefield, Giovanni Giuliano, Peter M. Waterhouse
Publicado en: bioRxiv, 2022, ISSN 2692-8205
Editor: Cold Spring Harbor
DOI: 10.1101/2022.12.30.521993

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