Exploring the Chemical Biology of Sequence Space via Picoliter Droplets

Publications

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution

Auteurs: Che Fai Alex Wong, Liisa van Vliet, Swapnil Vilas Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjørn Torger Stokke, Florian Hollfelder, Rahmi Lale
Publié dans: ACS Synthetic Biology, 2021, ISSN 2161-5063
Éditeur: American Chemical Society
DOI: 10.1021/acssynbio.1c00084

Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase

Auteurs: J. David Schnettler, Oskar James Klein, Tomasz S. Kaminski, Pierre-Yves Colin, Florian Hollfelder
Publié dans: J Am Chem Soc, 2023, Page(s) 1083–1096, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.2c10673

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

Auteurs: Maximilian Gantz, Stefanie Neun, Elliot J. Medcalf, Liisa D. van Vliet, Florian Hollfelder
Publié dans: Chemical Reviews, 2023, Page(s) ASAP, ISSN 0009-2665
Éditeur: American Chemical Society
DOI: 10.1021/acs.chemrev.2c00910

Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation

Auteurs: Clara Munger, Timo N. Kohler, Erin Slatery, Anna L. Ellermann, Sophie Bergmann, Christopher A. Penfold, Ioakeim Ampartzidis, Yutong Chen, Florian Hollfelder, Thorsten E. Boroviak
Publié dans: Development, 2022, Page(s) dev200263, ISSN 1477-9129
Éditeur: The company of biologists
DOI: 10.1242/dev.200263

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

Auteurs: Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Publié dans: Nature Communications, Numéro 11/1, 2020, Page(s) 6023, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-020-19687-9

Enzymatic N-Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases

Auteurs: Godwin A. Aleku, Gabriel R. Titchiner, George W. Roberts, Sasha R. Derrington, James R. Marshall, Florian Hollfelder, Nicholas J. Turner, and David Leys
Publié dans: ACS Sustainable Chem. Eng., Numéro 2168-0485, 2022, Page(s) 6794–6806, ISSN 2168-0485
Éditeur: American Chemical Society
DOI: 10.1021/acssuschemeng.2c01180

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture.

Auteurs: Knyphausen P, Rangel Pereira M, Brear P, Hyvönen M, Jermutus L, Hollfelder F.
Publié dans: Nature Communications, Numéro 14, 2023, Page(s) 768, ISSN 1608-3199
Éditeur: Nature
DOI: 10.1038/s41467-023-36099-7

Engineering the protein dynamics of an ancestral luciferase

Auteurs: Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Publié dans: Nature Communications, Numéro 12/1, 2021, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-021-23450-z

Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid

Auteurs: Berna Sozen, Andy L. Cox, Joachim De Jonghe, Min Bao, Florian Hollfelder, David M. Glover, Magdalena Zernicka-Goetz
Publié dans: Developmental Cell, Numéro 51/6, 2019, Page(s) 698-712.e8, ISSN 1534-5807
Éditeur: Cell Press
DOI: 10.1016/j.devcel.2019.11.014

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins

Auteurs: Laurens Lindenburg, Tuomas Huovinen, Kayleigh van de Wiel, Michael Herger, Michael R Snaith, Florian Hollfelder
Publié dans: Nucleic Acids Research, Numéro 48/11, 2020, Page(s) e63-e63, ISSN 0305-1048
Éditeur: Oxford University Press
DOI: 10.1093/nar/gkaa270

Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures

Auteurs: Vasileios Anagnostidis, Benjamin Sherlock, Jeremy Metz, Philip Mair, Florian Hollfelder, Fabrice Gielen
Publié dans: Lab on a Chip, Numéro 20/5, 2020, Page(s) 889-900, ISSN 1473-0197
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0lc00055h

Global fitness landscapes of the Shine-Dalgarno sequence

Auteurs: Syue-Ting Kuo, Ruey-Lin Jahn, Yuan-Ju Cheng, Yi-Lan Chen, Yun-Ju Lee, Florian Hollfelder, Jin-Der Wen, Hsin-Hung David Chou
Publié dans: Genome Research, Numéro 30/5, 2020, Page(s) 711-723, ISSN 1088-9051
Éditeur: Cold Spring Harbor Laboratory Press
DOI: 10.1101/gr.260182.119

Microfluidic platform for live cell imaging of 3D cultures with clone retrieval

Auteurs: Carla Mulas; Andrew C Hodgson; Timo N Kohler; Chibeza C. Agley; Florian Hollfelder; Austin Smith; Austin Smith; Kevin J. Chalut
Publié dans: Lab Chip, Numéro 9, 2020, Page(s) 2580-2591, ISSN 1473-0197
Éditeur: Royal Society of Chemistry
DOI: 10.1101/2020.02.17.952689

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays

Auteurs: Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, Florian Hollfelder
Publié dans: Analytical Chemistry, Numéro 93/4, 2021, Page(s) 2166-2173, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.0c03992

Investigating host-microbiome interactions by droplet based microfluidics

Auteurs: Alexandra S. Tauzin, Mariana Rangel Pereira, Liisa D. Van Vliet, Pierre-Yves Colin, Elisabeth Laville, Jeremy Esque, Sandrine Laguerre, Bernard Henrissat, Nicolas Terrapon, Vincent Lombard, Marion Leclerc, Joël Doré, Florian Hollfelder, Gabrielle Potocki-Veronese
Publié dans: Microbiome, Numéro 8/1, 2020, Page(s) 141, ISSN 2049-2618
Éditeur: BMC Spinger Nature
DOI: 10.1186/s40168-020-00911-z

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

Auteurs: Elliot J. Medcalf, Maximilian Gantz, Tomasz S. Kaminski*, and Florian Hollfelder*
Publié dans: Analytical Chemistry, 2023, Page(s) 4597–4604, ISSN 1608-3199
Éditeur: Nature Publishing
DOI: 10.1021/acs.analchem.2c04144

Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering

Auteurs: Thomas Fryer, Joel David Rogers, Christopher Mellor, Timo N. Kohler, Ralph Minter, Florian Hollfelder
Publié dans: ACS Central Science, 2022, Page(s) 1182–1195, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/acscentsci.2c00576

Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance.

Auteurs: Richter, Esther S; Link, Andreas; McGrath, John S; Sparrow, Raymond W; Gantz, Maximilian; Medcalf, Elliot J; Hollfelder, Florian; Franke, Thomas
Publié dans: Lab Chip, Numéro 6, 2023, Page(s) 195-202, ISSN 1473-0189
Éditeur: Royal society of Chemistry
DOI: 10.17863/cam.91468

Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro

Auteurs: Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan, Meng Zhu, Christos Kyprianou, Florian Hollfelder, Magdalena Zernicka-Goetz
Publié dans: Developmental Cell, Numéro 56/3, 2021, Page(s) 366-382.e9, ISSN 1534-5807
Éditeur: Cell Press
DOI: 10.1016/j.devcel.2020.12.004

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space

Auteurs: Christopher D. Bayer, Bert van Loo, Florian Hollfelder
Publié dans: ChemBioChem, Numéro 18/11, 2017, Page(s) 1001-1015, ISSN 1439-4227
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600657

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

Auteurs: Miriam Kaltenbach, Stephane Emond, Florian Hollfelder, Nobuhiko Tokuriki
Publié dans: PLOS Genetics, Numéro 12/10, 2016, Page(s) e1006305, ISSN 1553-7404
Éditeur: PLOS
DOI: 10.1371/journal.pgen.1006305

Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti : A Class I Sulfatase Specific for an Alkyl Sulfate Ester

Auteurs: Bert van Loo, Markus Schober, Eugene Valkov, Magdalena Heberlein, Erich Bornberg-Bauer, Kurt Faber, Marko Hyvönen, Florian Hollfelder
Publié dans: Journal of Molecular Biology, Numéro 430/7, 2018, Page(s) 1004-1023, ISSN 0022-2836
Éditeur: Academic Press
DOI: 10.1016/j.jmb.2018.02.010

Divergent synthesis of biflavonoids yields novel inhibitors of the aggregation of amyloid β (1–42)

Auteurs: Tze Han Sum, Tze Jing Sum, Súil Collins, Warren R. J. D. Galloway, David G. Twigg, Florian Hollfelder, David R. Spring
Publié dans: Organic & Biomolecular Chemistry, Numéro 15/21, 2017, Page(s) 4554-4570, ISSN 1477-0520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/C7OB00804J

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

Auteurs: Fabrice Gielen, Raphaelle Hours, Stephane Emond, Martin Fischlechner, Ursula Schell, Florian Hollfelder
Publié dans: Proceedings of the National Academy of Sciences, Numéro 113/47, 2016, Page(s) E7383-E7389, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.1606927113

Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset

Auteurs: Charlotte M. Miton, Stefanie Jonas, Gerhard Fischer, Fernanda Duarte, Mark F. Mohamed, Bert van Loo, Bálint Kintses, Shina C. L. Kamerlin, Nobuhiko Tokuriki, Marko Hyvönen, Florian Hollfelder
Publié dans: Proceedings of the National Academy of Sciences, Numéro 115/31, 2018, Page(s) E7293-E7302, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.1607817115

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets

Auteurs: Fabrice Gielen, Maren Butz, Eric J. Rees, Miklos Erdelyi, Tommaso Moschetti, Marko Hyvönen, Joshua B. Edel, Clemens F. Kaminski, Florian Hollfelder
Publié dans: Analytical Chemistry, Numéro 89/2, 2016, Page(s) 1092-1101, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.6b02528

Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase

Auteurs: Bert van Loo, Ryan Berry, Usa Boonyuen, Mark F. Mohamed, Marko Golicnik, Alvan C. Hengge, Florian Hollfelder
Publié dans: Biochemistry, Numéro 58/10, 2019, Page(s) 1363-1378, ISSN 0006-2960
Éditeur: American Chemical Society
DOI: 10.1021/acs.biochem.8b00996

Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily

Auteurs: Bert van Loo, Christopher D. Bayer, Gerhard Fischer, Stefanie Jonas, Eugene Valkov, Mark F. Mohamed, Anastassia Vorobieva, Celine Dutruel, Marko Hyvönen, Florian Hollfelder
Publié dans: Journal of the American Chemical Society, Numéro 141/1, 2018, Page(s) 370-387, ISSN 0002-7863
Éditeur: American Chemical Society
DOI: 10.1021/jacs.8b10290

Long‐Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation

Auteurs: Hans Kleine‐Brüggeney, Liisa D. van Vliet, Carla Mulas, Fabrice Gielen, Chibeza C. Agley, José C. R. Silva, Austin Smith, Kevin Chalut, Florian Hollfelder
Publié dans: Small, Numéro 15/5, 2019, Page(s) 1804576, ISSN 1613-6810
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201804576

Controlled oil/water partitioning of hydrophobic substrates extends the bioanalytical applications of droplet-based microfluidics

Auteurs: Tomas Buryska, Michal Vasina, Fabrice Gielen, Pavel Vanacek, Liisa van Vliet, Jan Jezek, Zdenek Pilat, Pavel Zemanek, Jiří Damborský, Florian Hollfelder, Zbyněk Prokop
Publié dans: Analytical Chemistry, 2019, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.9b01839

Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

Auteurs: Laurens H. Lindenburg, Teodors Pantelejevs, Fabrice Gielen, Pedro Zuazua-Villar, Maren Butz, Eric Rees, Clemens F. Kaminski, Jessica A. Downs, Marko Hyvönen, Florian Hollfelder
Publié dans: Proc Natl Acad Sci U S A ., 2021, Page(s) e2017708118, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.2017708118

High-throughput total RNA sequencing in single cells using VASA-seq

Auteurs: Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder & Alexander van Oudenaarden
Publié dans: Nature Biotechnology, 2022, Page(s) 1780–1793, ISSN 1087-0156
Éditeur: Nature Publishing Group
DOI: 10.1038/s41587-022-01361-8

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Auteurs: Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Publié dans: Nature Communications, Numéro 11/1, 2020, Page(s) 1, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-020-17061-3

Functional metagenomic screening identifies an unexpected β-glucuronidase

Auteurs: Stefanie Neun, Paul Brear, Eleanor Campbell, Theodora Tryfona, Kamel El Omari, Armin Wagner, Paul Dupree, Marko Hyvönen, Florian Hollfelder
Publié dans: Nature Chemical Biology, 2022, Page(s) 1096–1103, ISSN 1552-4450
Éditeur: Nature Publishing Group
DOI: 10.1038/s41589-022-01071-x

Embryo model completes gastrulation to neurulation and organogenesis

Auteurs: Gianluca Amadei, Charlotte E. Handford, Chengxiang Qiu, Joachim De Jonghe, Hannah Greenfeld, Martin Tran, Beth K. Martin, Dong-Yuan Chen, Alejandro Aguilera-Castrejon, Jacob H. Hanna, Michael B. Elowitz, Florian Hollfelder, Jay Shendure, David M. Glover & Magdalena Zernicka-Goetz
Publié dans: Nature, 2022, Page(s) 143–153, ISSN 1476-4687
Éditeur: Nature
DOI: 10.1038/s41586-022-05246-3

Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation

Auteurs: George S. Biggs, Oskar James Klein, Sarah L. Maslen, J. Mark Skehel, Trevor J. Rutherford, Stefan M. V. Freund, Florian Hollfelder, Sally R. Boss, Paul D. Barker
Publié dans: Angewandte Chemie International Edition, Numéro 60/19, 2021, Page(s) 10919-10927, ISSN 1433-7851
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015834

In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region

Auteurs: Kalliopi Skamaki, Stephane Emond, Matthieu Chodorge, John Andrews, D. Gareth Rees, Daniel Cannon, Bojana Popovic, Andrew Buchanan, Ralph R. Minter, Florian Hollfelder
Publié dans: Proceedings of the National Academy of Sciences, Numéro 117/44, 2020, Page(s) 27307-27318, ISSN 0027-8424
Éditeur: National Academy of Sciences
DOI: 10.1073/pnas.2002954117

Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells

Auteurs: Magdalena Schindler, Dylan Siriwardena, Timo N. Kohler, Anna L. Ellermann, Erin Slatery, Clara Munger, Florian Hollfelder, Thorsten E. Boroviak
Publié dans: Stem Cell Reports, Numéro 16/5, 2021, Page(s) 1347-1362, ISSN 2213-6711
Éditeur: Cell Press
DOI: 10.1016/j.stemcr.2021.04.009

Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

Auteurs: Remkes A. Scheele, Laurens H. Lindenburg, Maya Petek, Markus Schober, Kevin N. Dalby, Florian Hollfelder
Publié dans: Nature Communications, 2022, Page(s) 844, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-022-28396-4

Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay

Auteurs: Miriam Klaus,Paul Jannis Zurek, Tomasz S. Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Publié dans: ChemBioChem, 2021, Page(s) 3292-3299, ISSN 1439-7633
Éditeur: Wiley
DOI: 10.1002/cbic.202100322

Spatial profiling of early primate gastrulation in utero

Auteurs: Sophie Bergmann, Christopher A. Penfold, Erin Slatery, Dylan Siriwardena, Charis Drummer, Stephen Clark, Stanley E. Strawbridge, Keiko Kishimoto, Alice Vickers, Mukul Tewary, Timo N. Kohler, Florian Hollfelder, Wolf Reik, Erika Sasaki, Rüdiger Behr & Thorsten E. Boroviak
Publié dans: Nature, 2022, Page(s) 136–143, ISSN 1476-4687
Éditeur: Nature
DOI: 10.1038/s41586-022-04953-1

Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation

Auteurs: Lucía Cordero-Espinoza, Anna M. Dowbaj, Timo N. Kohler, Bernhard Strauss, Olga Sarlidou, German Belenguer, Clare Pacini, Nuno P. Martins, Ross Dobie, John R. Wilson-Kanamori, Richard Butler, Nicole Prior, Palle Serup, Florian Jug, Neil C. Henderson, Florian Hollfelder, Meritxell Huch
Publié dans: Cell Stem Cell, 2021, ISSN 1934-5909
Éditeur: Cell Press
DOI: 10.1016/j.stem.2021.07.002

Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

Auteurs: Min Bao, Jake Cornwall-Scoones, Estefania Sanchez-Vasquez, Dong-Yuan Chen, Joachim De Jonghe, Shahriar Shadkhoo, Florian Hollfelder, Matt Thomson, David M. Glover & Magdalena Zernicka-Goetz
Publié dans: Nature Cell Biology, 2022, Page(s) 1341–1349, ISSN 1465-7392
Éditeur: Nature Publishing Group
DOI: 10.1038/s41556-022-00984-y

High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform

Auteurs: Stefanie Neun, Liisa van Vliet, Florian Hollfelder, and Fabrice Gielen
Publié dans: Analytical Chemistry, 2022, Page(s) 16701–16710, ISSN 1608-3199
Éditeur: ACS
DOI: 10.1021/acs.analchem.2c03164

Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation

Auteurs: Paul Jannis Zurek, Raphaëlle Hours, Ursula Schell, Ahir Pushpanath, Florian Hollfelder
Publié dans: Lab on a Chip, Numéro 21/1, 2021, Page(s) 163-173, ISSN 1473-0197
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0lc00830c

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

Auteurs: Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Publié dans: bioRxiv, 2023, Page(s) n/a, ISSN 2692-8205
Éditeur: CSH Press
DOI: 10.1101/2023.01.12.523500

Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

Auteurs: Katrin Fischer, Aleksei Lulla, Tsz Y So, Pehuén Pereyra-Gerber, Matthew I. J. Raybould, Timo N. Kohler, Tomasz S. Kaminski, Juan Carlos Yam-Puc, Robert Hughes, Florian Leiß-Maier, Paul Brear, Nicholas J. Matheson, Charlotte M. Deane, Marko Hyvönen, James E. D. Thaventhiran, Florian Hollfelder
Publié dans: bioRxiv, 2023, Page(s) n/a, ISSN 2692-8205
Éditeur: Cold Spring Harbour Press
DOI: 10.1101/2023.01.10.523494

Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins

Auteurs: J. David Schnettler, Michael S. Wang, Maximilian Gantz, Christina Karas, Florian Hollfelder, Michael H. Hecht
Publié dans: BioRxiv, 2023, Page(s) n/a, ISSN 2692-8205
Éditeur: CSH Press
DOI: 10.1101/2023.02.13.528392

Versatile Product Detection via Coupled Assays for Ultra-high-throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets

Auteurs: Simon Ladeveze, Paul J. Zurek, Tomasz S. Kaminski, Stephane Emond, Florian Hollfelder
Publié dans: Biorxiv, 2023, Page(s) n/a, ISSN 2692-8205
Éditeur: CDH Laboratory
DOI: 10.1101/2023.03.29.534725

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

Auteurs: Bert van Loo, Magdalena Heberlein, Philip Mair, Anastasia Zinchenko, Jan Schüürmann, Bernard D. G. Eenink, Josephin M. Holstein, Carina Dilkaute, Joachim Jose, Florian Hollfelder, Erich Bornberg-Bauer
Publié dans: ACS Synthetic Biology, Numéro 8/12, 2019, Page(s) 2690-2700, ISSN 2161-5063
Éditeur: American Chemical Society
DOI: 10.1021/acssynbio.9b00274

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MODULAR MICROFLUIDIC METHODS AND DEVICES

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