“Tracking nano-bioprocesses using Super-Resolution Microscopy Techniques”

Livrables

Minute conference calls for coordination of visiting periods

Minute conference calls for coordination of visiting periods.

A set of DNA-based fluorescent TF-beacons for TEAD and NF-kB inhibition; Characterization of TEAD/YAP behaviour during mammary cancer progression

A set of DNA-based fluorescent TF-beacons for TEAD and NF-kB inhibition. Characterization of TEAD/YAP behaviour during mammary cancer progression.

Complete the synthesis and characterization of siRNA-loaded nanobubble and nanogels

Lead Partner: UOM Complete the synthesis and characterization of siRNA-loaded nanobubble and nanogels.

Lecture notes and conference presentations

Lead Partner: UOM Lecture notes and conference presentations.

Definition of the best conditions to obtain and enrich iPSCs lines expressing YAP-GFP construct and its mutants; Definition of the domains of YAP protein responsible for its acknowledged mechanosensor activity

Definition of the best conditions to obtain and enrich iPSCs lines expressing YAP-GFP construct and its mutants. Definition of the domains of YAP protein responsible for its acknowledged mechanosensor activity.

Lecture notes on advanced microscopy methods and technologies

Lecture notes on advanced microscopy methods and technologies.

Curriculum of interdisciplinary studies on innovation strategies

Curriculum of interdisciplinary studies on innovation strategies.

"Lecture notes on ""Role of mechanotransduction systems during stem cell differentiation"" and “ Advanced microscopy for protein tracking”"

"Lecture notes on ""Role of mechanotransduction systems during stem cell differentiation"" and “ Advanced microscopy for protein tracking”."

Submitted grant proposals to European Community, NSF and NIH (USA), ARC

Submitted grant proposals to European Community, NSF and NIH (USA), ARC.

Proceedings and teaching material concerning EU grants

Proceedings and teaching material concerning EU grants.

Training young scientist on biochemistry and molecular biology methods in the laboratory; Training young scientist on experimental methods for studying YAP/TEAD and NFkB

Training young scientist on biochemistry and molecular biology methods in the laboratory. Training young scientist on experimental methods for studying YAP/TEAD and NFkB.

Support research giving access to most advanced technologies in the field of cellular imaging, data analysis and image processing available at IIT and Nikon Imaging Centre (NIC@IIT)

Support research giving access to most advanced technologies in the field of cellular imaging, data analysis and image processing available at IIT and Nikon Imaging Centre (NIC@IIT).

Training young scientist on experimental methods for studying YAP protein and protein mechanosensor; Training young scientist on experimental advanced microscopy-AFM techniques

Training young scientist on experimental methods for studying YAP protein and protein mechanosensor. Training young scientist on experimental advanced microscopy-AFM techniques.

"Lecture notes on ""Role of mechanotransduction systems during stem cell differentiation"""

"Lecture notes on ""Role of mechanotransduction systems during stem cell differentiation""."

Serve as a training platform providing courses, individual instruction, and consulting on STED, STORM, SIM, FCS-STED and FLIM

Serve as a training platform providing courses, individual instruction, and consulting on STED, STORM, SIM, FCS-STED and FLIM.

Training ESR on experimental methods for studying the behaviour of DNA-based conformational switching probes in solution; Training ESR on experimental methods for engineering and characterization of nanocarriers

Lead Partner: UC Training ESR on experimental methods for studying the behaviour of DNA-based conformational switching probes in solution. Training ESR on experimental methods for engineering and characterization of nanocarriers.

Seminars held at industries and SME

Seminars held at industries and SME.

Training young scientist on experimental methods for nanobubbles and nanogels preparation; Training young scientist on experimental methods for studying the physico-chemical properties of siRNA loaded nanocarriers

Lead Partner: UOM Training young scientist on experimental methods for nanobubbles and nanogels preparation. Training young scientist on experimental methods for studying the physico-chemical properties of siRNA loaded nanocarriers.

Training young scientist on the use of STED, STORM, SIM, FCS-STED and FLIM

Training young scientist on the use of STED, STORM, SIM, FCS-STED and FLIM.

Publications

Probing transcription factor binding activity and downstream gene silencing in living cells with a DNA nanoswitch

Auteurs: Alessandro Bertucci, Junling Guo, Nicolas Oppmann, Agata Glab, Francesco Ricci, Frank Caruso, Francesca Cavalieri
Publié dans: Nanoscale, Numéro 10/4, 2018, Page(s) 2034-2044, ISSN 2040-3364
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c7nr07814e

Tuning the Morphology of Nanostructured Peptide Films by the Introduction of a Secondary Structure Conformational Constraint: A Case Study of Hierarchical Self-Assembly

Auteurs: Marta De Zotti, Beatrice Muzzi, Emanuela Gatto, Benedetta Di Napoli, Claudia Mazzuca, Antonio Palleschi, Ernesto Placidi, Fernando Formaggio, Claudio Toniolo, Mariano Venanzi
Publié dans: The Journal of Physical Chemistry B, Numéro 122/24, 2018, Page(s) 6305-6313, ISSN 1520-6106
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpcb.8b01877

Hybrid Conjugates Formed between Gold Nanoparticles and an Amyloidogenic Diphenylalanine-Cysteine Peptide

Auteurs: Juliane N. B. D. Pelin, Emanuela Gatto, Mariano Venanzi, Francesca Cavalieri, Cristiano L. P. Oliveira, Herculano Martinho, Emerson R. Silva, Andrea M. Aguilar, Juliana S. Souza, Wendel A. Alves
Publié dans: ChemistrySelect, Numéro 3/24, 2018, Page(s) 6756-6765, ISSN 2365-6549
Éditeur: Wiley
DOI: 10.1002/slct.201801345

Controlling the Formation of Peptide Films: Fully Developed Helical Peptides are Required to Obtain a Homogenous Coating over a Large Area

Auteurs: Marta De Zotti, Gabriele Corvi, Emanuela Gatto, Benedetta Di Napoli, Claudia Mazzuca, Antonio Palleschi, Ernesto Placidi, Barbara Biondi, Marco Crisma, Fernando Formaggio, Claudio Toniolo, Mariano Venanzi
Publié dans: ChemPlusChem, Numéro 84/11, 2019, Page(s) 1688-1696, ISSN 2192-6506
Éditeur: Wiley-VCH Verlag
DOI: 10.1002/cplu.201900456

Tuning the morphology of mesoscopic structures of porphyrin macrocycles functionalized by an antimicrobial peptide

Auteurs: Rita Cimino, Elisa Grelloni, Gabriele Magna, Donato Monti, Manuela Stefanelli, Emanuela Gatto, Ernesto Placidi, Francesca Biscaglia, Marina Gobbo, Mariano Venanzi
Publié dans: Journal of Porphyrins and Phthalocyanines, 2020, Page(s) A-I, ISSN 1088-4246
Éditeur: Society of Porphyrins and Phthalocyanines
DOI: 10.1142/s1088424619502006

Hierarchical Self‐Assembly of Peptides and its Applications in Bionanotechnology

Auteurs: Barbara B. Gerbelli, Sandra V. Vassiliades, Jose E. U. Rojas, Juliane N. B. D. Pelin, Rodrigo S. N. Mancini, Wallace S. G. Pereira, Andrea M. Aguilar, Mariano Venanzi, Francesca Cavalieri, Francesca Giuntini, Wendel A. Alves
Publié dans: Macromolecular Chemistry and Physics, Numéro 220/14, 2019, Page(s) 1900085, ISSN 1022-1352
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/macp.201900085

Sound-driven dissipative self-assembly of aromatic biomolecules into functional nanoparticles

Auteurs: Sukhvir Kaur Bhangu, Gianfranco Bocchinfuso, Muthupandian Ashokkumar, Francesca Cavalieri
Publié dans: Nanoscale Horizons, 2020, ISSN 2055-6756
Éditeur: rcs
DOI: 10.1039/c9nh00611g

Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles

Auteurs: Pietro Pacchin Tomanin, Jiajing Zhou, Alessia Amodio, Rita Cimino, Agata Glab, Francesca Cavalieri, Frank Caruso
Publié dans: Journal of Materials Chemistry B, 2020, ISSN 2050-7518
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0tb00299b

Combining Nanomaterials and Developmental Pathways to Design New Treatments for Cardiac Regeneration: The Pulsing Heart of Advanced Therapies

Auteurs: Marco Cassani, Soraia Fernandes, Jan Vrbsky, Ece Ergir, Francesca Cavalieri, Giancarlo Forte
Publié dans: Frontiers in Bioengineering and Biotechnology, Numéro 8, 2020, ISSN 2296-4185
Éditeur: Frontiers
DOI: 10.3389/fbioe.2020.00323

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