Objective
Metagenomes comprise enormous reservoirs coding for proteins with useful activities. Unfortunately, harvesting this reservoir is difficult, because useful candidates are rare and hidden in an overwhelming majority of irrelevant genes. Screening campaigns of metagenomic libraries thus require massive capital-expenditure for robotic systems and much manpower, making them expensive, slow and available to very few users. To enable valorisation of the potential of the metagenome, this project assembles an interdisciplinary and intersectoral consortium that will integrate a range of technologies into a platform designed to beat the odds of identifying library hits faster, more efficiently and by a wider user base. Exploration and exploitation of the metagenome will be made faster and more successful by (i) ultrahigh-throughput screening in picoliter droplets that dramatically lowers the cost per assay to well below 0.01 cents and allows throughput of 10e7 assays per hour; (ii) workflows that streamline and increase the yield of library construction and functional expression and (iii) workflows for efficient bioinformatic analysis of hits based on user-friendly software solutions for metagenome analysis. Emphasis is put on technologies that are straightforwardly implemented in non-specialist labs, maximising the impact of METAFLUIDICS. This platform will be used to identify enzymes for biosynthesis of therapeutic small molecules, for green bioenergy conversion, bioremediation, food chemistry and other industrial applications
Fields of science
- natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidics
- natural sciencescomputer and information sciencessoftware
- engineering and technologyenvironmental biotechnologybioremediation
- natural sciencesbiological sciencesmicrobiology
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programme(s)
Funding Scheme
RIA - Research and Innovation actionCoordinator
28049 Madrid
Spain
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Participants (15)
8000 Aarhus
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18100 ARMILLA GRANADA
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The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
CB4 3QU Cambridge
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The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
NE49 9HN Haltwhistle
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The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
CB2 1TN Cambridge
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2880 Bagsvaerd
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31077 TOULOUSE CEDEX 4
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Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.
75007 Paris
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3004 517 Coimbra
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03690 Alicante
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28006 Madrid
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Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.
28850 Torrejon De Ardoz Madrid
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7491 Trondheim
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Participation ended
7034 Trondheim
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7034 Trondheim
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