Engineering of a minimal bacterial therapeutic chassis

Project Information

MYCOCHASSIS

Grant agreement ID: 670216

DOI 10.3030/670216

Project closed

EC signature date 14 October 2015

Start date 1 November 2015

End date 31 October 2021

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 454 522,00

EU contribution € 2 454 522,00

Coordinated by

FUNDACIO CENTRE DE REGULACIO GENOMICA
Spain

Publications

Peer reviewed articles (14)

Engineering a genome-reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo

Author(s): Garrido, V; Pinero-Lambea, C; Rodriguez-Arce, I; Paetzold, B; Ferrar, T; Weber, M; Garcia-Ramallo, E; Gallo, C; Collantes, M; Penuelas, I; Serrano, L; Grillo, MJ; Lluch-Senar, M
Published in: Molecular Systems Biology, 2021, ISSN 1744-4292
Publisher: Nature Publishing Group
DOI: 10.15252/msb.202010145

A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants

Author(s): Eva Yus, Jae-Seong Yang, Adrià Sogues, Luis Serrano
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-00239-7

Alternative transcriptional regulation in genome-reduced bacteria

Author(s): Samuel Miravet-Verde, Verónica Lloréns-Rico, Luis Serrano
Published in: Current Opinion in Microbiology, Issue 39, 2017, Page(s) 89-95, ISSN 1369-5274
Publisher: Elsevier BV
DOI: 10.1016/j.mib.2017.10.022

Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Author(s): Marie Trussart, Eva Yus, Sira Martinez, Davide Baù, Yuhei O. Tahara, Thomas Pengo, Michael Widjaja, Simon Kretschmer, Jim Swoger, Steven Djordjevic, Lynne Turnbull, Cynthia Whitchurch, Makoto Miyata, Marc A. Marti-Renom, Maria Lluch-Senar, Luís Serrano
Published in: Nature Communications, Issue 8, 2017, Page(s) 14665, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms14665

Assessing the limits of restraint-based 3D modeling of genomes and genomic domains

Author(s): M. Trussart, F. Serra, D. Bau, I. Junier, L. Serrano, M. A. Marti-Renom
Published in: Nucleic Acids Research, Issue 43/7, 2015, Page(s) 3465-3477, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkv221

Bacterial antisense RNAs are mainly the product of transcriptional noise

Author(s): V. Llorens-Rico, J. Cano, T. Kamminga, R. Gil, A. Latorre, W.-H. Chen, P. Bork, J. I. Glass, L. Serrano, M. Lluch-Senar
Published in: Science Advances, Issue 2/3, 2016, Page(s) e1501363-e1501363, ISSN 2375-2548
Publisher: Science Advances
DOI: 10.1126/sciadv.1501363

Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium

Author(s): M. Lluch-Senar, J. Delgado, W.-H. Chen, V. Llorens-Rico, F. J. O'Reilly, J. A. Wodke, E. B. Unal, E. Yus, S. Martinez, R. J. Nichols, T. Ferrar, A. Vivancos, A. Schmeisky, J. Stulke, V. van Noort, A.-C. Gavin, P. Bork, L. Serrano
Published in: Molecular Systems Biology, Issue 11/1, 2015, Page(s) 780-780, ISSN 1744-4292
Publisher: Nature Publishing Group
DOI: 10.15252/msb.20145558

Rescuing discarded spectra: Full comprehensive analysis of a minimal proteome

Author(s): Maria Lluch-Senar, Francesco M. Mancuso, H?ctor Climente-Gonz?lez, Marcia I. Pe?a-Paz, Eduard Sabido, Luis Serrano
Published in: PROTEOMICS, Issue 16/4, 2016, Page(s) 554-563, ISSN 1615-9853
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/pmic.201500187

Integration of multi-omics data of a genome-reduced bacterium: Prevalence of post-transcriptional regulation and its correlation with protein abundances

Author(s): Wei-Hua Chen, Vera van?Noort, Maria Lluch-Senar, Marco L. Hennrich, Judith A. H.?Wodke, Eva Yus, Andreu Alib?s, Guglielmo Roma, Daniel R. Mende, Christina Pesavento, Athanasios Typas, Anne-Claude Gavin, Luis Serrano, Peer Bork
Published in: Nucleic Acids Research, Issue 44/3, 2016, Page(s) 1192-1202, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkw004

Distinguishing between productive and abortive promoters using a random forest classifier in Mycoplasma pneumoniae

Author(s): V. Llorens-Rico, M. Lluch-Senar, L. Serrano
Published in: Nucleic Acids Research, Issue 43/7, 2015, Page(s) 3442-3453, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkv170

Insights into the Mechanisms of Basal Coordination of Transcription Using a Genome-Reduced Bacterium

Author(s): Ivan Junier, E.?Besray Unal, Eva Yus, Ver?nica Llor?ns-Rico, Luis Serrano
Published in: Cell Systems, Issue 2/6, 2016, Page(s) 391-401, ISSN 2405-4712
Publisher: Cell Systems
DOI: 10.1016/j.cels.2016.04.015

Comparative ?-omics? in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors

Author(s): Maria Lluch-Senar, Luca Cozzuto, Jaime Cano, Javier Delgado, Ver?nica Ll?rens-Rico, Sabine Pereyre, C?cile Bebear, Luis Serrano
Published in: PLOS ONE, Issue 10/9, 2015, Page(s) e0137354, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0137354

Impact of C‐terminal amino acid composition on protein expression in bacteria

Author(s): Marc Weber, Raul Burgos, Eva Yus, Jae‐Seong Yang, Maria Lluch‐Senar, Luis Serrano
Published in: Molecular Systems Biology, Issue 16/5, 2020, ISSN 1744-4292
Publisher: Nature Publishing Group
DOI: 10.15252/msb.20199208

Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control

Author(s): Burgos, R; Weber, M; Gallo, C; Lluch-Senar, M; Serrano, L
Published in: Iscience, 2021, ISSN 2589-0042
Publisher: Elsevier
DOI: 10.1016/j.isci.2021.102985