Studying the bricks of microbial cities: characterization and structural properties of exopolysaccharides and their interaction with proteins and cations in anammox granular sludge

Projektinformationen

POLIS

ID Finanzhilfevereinbarung: 661429

DOI 10.3030/661429

Projekt abgeschlossen

EK-Unterschriftsdatum 22 September 2015

Startdatum 1 Dezember 2015

Enddatum 30 November 2017

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 168 277,20

EU-Beitrag € 168 277,20

Koordiniert durch

POLITECNICO DI MILANO
Italy

Ziel

The anaerobic ammonium oxidation (anammox) is an innovative technology for the removal of nitrogen from wastewater (ww) saving 60% of aeration and 100% of organic carbon. Its potential application to municipal ww allow a complete redesign of the present energy-consuming into an energy-yielding ww treatment plant. Due to the slow growth rate of anammox bacteria, their retention in the system, and therefore granule stability, is the main concern of this process. Granular stability is closely related to the properties of their exopolysaccharides (PS) and their interactions with proteins and mono-divalent cations. In the proposed research integrated analytical methods (UV-visible, FT-IR, MALDI-TOF MS, NMR, (pyrolysis)GC-MS, HPLC) will be used to investigate functional PS and their fine structures, unrevealing the mechanisms involved in anammox granular sludge formation and stability. Modern rheological (AOS, LLCT and Microrheology combined with CLSM), light scattering (DLS, SLS) and microscopy techniques (AFM, FT-IR, CLSM) will be used to investigate their interactions with proteins and cations. PS will be isolated from lab- and full-scale reactors. The definition of standardized extraction (loosely/tightly bound-EPS, LB/TB-EPS) and analytical methods will bridge the existing gap for efficient and meaningful comparison of the results from this and future research. The methods set up here, will largely contribute to increase the scientific significance of PS studies in anammox granular sludge area as well as facilitate the entire biofilm research field. Studies on biofouling and biocorrosion will also benefit from the high-quality of the information and basic knowledge acquired. This research will strengthen the EU leadership in anammox granular sludge technology also improving EU scientific excellence in PS research area and provides the researcher an advanced level of multidisciplinary training, opening concrete perspectives for his career development.

Wissenschaftliches Gebiet

CORDIS klassifiziert Projekte mit EuroSciVoc, einer mehrsprachigen Taxonomie der Wissenschaftsbereiche, durch einen halbautomatischen Prozess, der auf Verfahren der Verarbeitung natürlicher Sprache beruht.

• natural sciences › biological sciences › biochemistry › biomolecules › proteins › proteomics
• natural sciences › biological sciences › microbiology › bacteriology
• natural sciences › physical sciences › optics › spectroscopy › emission spectroscopy
• natural sciences › chemical sciences › analytical chemistry › calorimetry
• natural sciences › biological sciences › biochemistry › biomolecules › carbohydrates

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2014

Finanzierungsplan

MSCA-IF-EF-ST - Standard EF

Koordinator

POLITECNICO DI MILANO

• Netto-EU-Beitrag: € 168 277,20
• Adresse: PIAZZA LEONARDO DA VINCI 32 ; 20133 Milano ; Italien
• Region: Nord-Ovest › Lombardia › Milano
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 168 277,20