Mechanobiology of nuclear import of transcription factors modeled within a bioengineered stem cell niche.

Project Information

NICHOID

Grant agreement ID: 646990

DOI 10.3030/646990

Project closed

EC signature date 1 April 2015

Start date 1 May 2015

End date 31 July 2020

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 903 330,00

EU contribution € 1 903 330,00

Coordinated by

POLITECNICO DI MILANO
Italy

Publications

Peer reviewed articles (27)

Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction

Author(s): Federica Rey, Cecilia Pandini, Bianca Barzaghini, Letizia Messa, Toniella Giallongo, Orietta Pansarasa, Stella Gagliardi, Matteo Brilli, Gian Vincenzo Zuccotti, Cristina Cereda, Manuela Teresa Raimondi, Stephana Carelli
Published in: International Journal of Molecular Sciences, Issue 21/18, 2020, Page(s) 6775, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21186775

Tissue engineering and regenerative medicine strategies for the female breast

Author(s): Claudio Conci, Lorenzo Bennati, Chiara Bregoli, Federica Buccino, Francesca Danielli, Michela Gallan, Ereza Gjini, Manuela T. Raimondi
Published in: Journal of Tissue Engineering and Regenerative Medicine, Issue 14/2, 2020, Page(s) 369-387, ISSN 1932-6254
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/term.2999

Effect of the 3D Artificial Nichoid on the Morphology and Mechanobiological Response of Mesenchymal Stem Cells Cultured In Vitro

Author(s): Andrea Remuzzi, Barbara Bonandrini, Matteo Tironi, Lorena Longaretti, Marina Figliuzzi, Sara Conti, Tommaso Zandrini, Roberto Osellame, Giulio Cerullo, Manuela Teresa Raimondi
Published in: Cells, Issue 9/8, 2020, Page(s) 1873, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells9081873

Neural precursors cells expanded in a 3D micro-engineered niche present enhanced therapeutic efficacy in vivo

Author(s): Stephana Carelli, Toniella Giallongo, Federica Rey, Bianca Barzaghini, Tommaso Zandrini, Andrea Pulcinelli, Riccardo Nardomarino, Giulio Cerullo, Roberto Osellame, Cristina Cereda, Gian Vincenzo Zuccotti, Manuela Teresa Raimondi
Published in: Nanotheranostics, Issue 5/1, 2021, Page(s) 8-26, ISSN 2206-7418
Publisher: Ivyspring International Publisher Pty Ltd
DOI: 10.7150/ntno.50633

iPS, organoids and 3D models as advanced tools for in vitro toxicology

Author(s): Nathalie Steimberg
Published in: ALTEX, 2020, Page(s) 136-140, ISSN 1868-596X
Publisher: Springer Nature
DOI: 10.14573/altex.1911071

Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging

Author(s): Valentina Parodi, Emanuela Jacchetti, Roberto Osellame, Giulio Cerullo, Dario Polli, Manuela Teresa Raimondi
Published in: Frontiers in Bioengineering and Biotechnology, Issue 8, 2020, ISSN 2296-4185
Publisher: Frontiers Media SA
DOI: 10.3389/fbioe.2020.585363

Advantages and limitations of a supernegative GFP in facilitating MyoD intracellular tracking

Author(s): Lucia Boeri, Emanuela Jacchetti, Monica Soncini, Alessandro Negro, Diego Albani, Manuela Teresa Raimondi
Published in: Methods and Applications in Fluorescence, Issue 8/2, 2020, Page(s) 025007, ISSN 2050-6120
Publisher: IOP publications
DOI: 10.1088/2050-6120/ab797c

Bioengineering tools to speed up the discovery and preclinical testing of vaccines for SARS-CoV-2 and therapeutic agents for COVID-19

Author(s): Manuela Teresa Raimondi, Francesca Donnaloja, Bianca Barzaghini, Alberto Bocconi, Claudio Conci, Valentina Parodi, Emanuela Jacchetti, Stephana Carelli
Published in: Theranostics, Issue 10/16, 2020, Page(s) 7034-7052, ISSN 1838-7640
Publisher: Ivyspring International Publisher
DOI: 10.7150/thno.47406

Natural and Synthetic Polymers for Bone Scaffolds Optimization

Author(s): Francesca Donnaloja, Emanuela Jacchetti, Monica Soncini, Manuela T. Raimondi
Published in: Polymers, Issue 12/4, 2020, Page(s) 905, ISSN 2073-4360
Publisher: Molecular Diversity Preservation International
DOI: 10.3390/polym12040905

A poroelastic mixture model of mechanobiological processes in biomass growth: theory and application to tissue engineering

Author(s): Riccardo Sacco, Paola Causin, Chiara Lelli, Manuela T. Raimondi
Published in: Meccanica, Issue 52/14, 2017, Page(s) 3273-3297, ISSN 0025-6455
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s11012-017-0638-9

Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells

Author(s): Davide Ricci, Michele Nava, Tommaso Zandrini, Giulio Cerullo, Manuela Raimondi, Roberto Osellame
Published in: Materials, Issue 10/1, 2017, Page(s) 66, ISSN 1996-1944
Publisher: MDPI Open Access Publishing
DOI: 10.3390/ma10010066

An experimental-numerical investigation on the effects of macroporous scaffold geometry on cell culture parameters

Author(s): Hadis Eghbali, Michele M. Nava, Gabriella Leonardi, Davod Mohebbi-Kalhori, Roberto Sebastiano, Abdolreza Samimi, Manuela T. Raimondi
Published in: The International Journal of Artificial Organs, Issue 40/4, 2017, Page(s) 185-195, ISSN 0391-3988
Publisher: Wichtig Editore
DOI: 10.5301/ijao.5000554

Human bone perivascular niche-on-a-chip for studying metastatic colonization

Author(s): Alessandro Marturano-Kruik, Michele Maria Nava, Keith Yeager, Alan Chramiec, Luke Hao, Samuel Robinson, Edward Guo, Manuela Teresa Raimondi, Gordana Vunjak-Novakovic
Published in: Proceedings of the National Academy of Sciences, Issue 115/6, 2018, Page(s) 1256-1261, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1714282115

Oxygen measurement in interstitially perfused cellularized constructs cultured in a miniaturized bioreactor

Author(s): Manuela T. Raimondi, Carmen Giordano, Riccardo Pietrabissa
Published in: Journal of Applied Biomaterials & Functional Materials, Issue 13/4, 2015, Page(s) 0-0, ISSN 2280-8000
Publisher: Wichtig Editore s.r.l.
DOI: 10.5301/jabfm.5000246

Synthetic niche substrates engineered via two-photon laser polymerization for the expansion of human mesenchymal stromal cells

Author(s): Michele M. Nava, Nunzia Di Maggio, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Ivan Martin, Manuela T. Raimondi
Published in: Journal of Tissue Engineering and Regenerative Medicine, 2016, ISSN 1932-6254
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/term.2187

Computational prediction of strain-dependent diffusion of transcription factors through the cell nucleus

Author(s): Michele M. Nava, Roberto Fedele, Manuela T. Raimondi
Published in: Biomechanics and Modeling in Mechanobiology, Issue 15/4, 2016, Page(s) 983-993, ISSN 1617-7959
Publisher: Springer Verlag
DOI: 10.1007/s10237-015-0737-2

Hollow fiber bioreactor technology for tissue engineering applications

Author(s): Hadis Eghbali, Michele M. Nava, Davod Mohebbi-Kalhori, Manuela T. Raimondi
Published in: The International Journal of Artificial Organs, Issue 39/1, 2016, Page(s) 1-15, ISSN 0391-3988
Publisher: Wichtig Editore
DOI: 10.5301/ijao.5000466

Distributed and Lumped Parameter Models for the Characterization of High Throughput Bioreactors

Author(s): Laura Iannetti, Giovanna D’Urso, Gioacchino Conoscenti, Elena Cutrì, Rocky S. Tuan, Manuela T. Raimondi, Riccardo Gottardi, Paolo Zunino
Published in: PLOS ONE, Issue 11/9, 2016, Page(s) e0162774, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0162774

Two-photon polymerized “nichoid” substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions

Author(s): Michele M. Nava, Alessio Piuma, Marina Figliuzzi, Irene Cattaneo, Barbara Bonandrini, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Andrea Remuzzi, Manuela T. Raimondi
Published in: Stem Cell Research & Therapy, Issue 7/1, 2016, ISSN 1757-6512
Publisher: BioMed Central
DOI: 10.1186/s13287-016-0387-z

Optimization of a 3D Dynamic Culturing System for In Vitro Modeling of Frontotemporal Neurodegeneration-Relevant Pathologic Features

Author(s): Marta Tunesi, Federica Fusco, Fabio Fiordaliso, Alessandro Corbelli, Gloria Biella, Manuela T. Raimondi
Published in: Frontiers in Aging Neuroscience, Issue 8, 2016, ISSN 1663-4365
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnagi.2016.00146

Modeling of the mechano-chemical behaviour of the nuclear pore complex: current research and perspectives

Author(s): Alberto Garcia, Jose F. Rodriguez Matas, Manuela T. Raimondi
Published in: Integrative Biology, Issue 8/10, 2016, Page(s) 1011-1021, ISSN 1757-9694
Publisher: Royal Society of Chemistry
DOI: 10.1039/c6ib00153j

Secretome released from hydrogel-embedded adipose mesenchymal stem cells protects against the Parkinson’s disease related toxin 6-hydroxydopamine

Author(s): Armando Chierchia, Nino Chirico, Lucia Boeri, Ilaria Raimondi, Giovanni A. Riva, Manuela Teresa Raimondi, Marta Tunesi, Carmen Giordano, Gianluigi Forloni, Diego Albani
Published in: European Journal of Pharmaceutics and Biopharmaceutics, Issue 121, 2017, Page(s) 113-120, ISSN 0939-6411
Publisher: Elsevier BV
DOI: 10.1016/j.ejpb.2017.09.014

Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation

Author(s): Lucia Boeri, Diego Albani, Manuela Teresa Raimondi, Emanuela Jacchetti
Published in: Biophysical Reviews, Issue 11/5, 2019, Page(s) 817-831, ISSN 1867-2450
Publisher: Springer Verlag
DOI: 10.1007/s12551-019-00594-3

The Effect of Cell Morphology on the Permeability of the Nuclear Envelope to Diffusive Factors

Author(s): Alberto García-González, Emanuela Jacchetti, Roberto Marotta, Marta Tunesi, José F. Rodríguez Matas, Manuela T. Raimondi
Published in: Frontiers in Physiology, Issue 9, 2018, ISSN 1664-042X
Publisher: Frontiers Research Foundation
DOI: 10.3389/fphys.2018.00925

Mechanosensing at the Nuclear Envelope by Nuclear Pore Complex Stretch Activation and Its Effect in Physiology and Pathology

Author(s): F. Donnaloja, E. Jacchetti, M. Soncini, M. T. Raimondi
Published in: Frontiers in Physiology, Issue 10, 2019, ISSN 1664-042X
Publisher: Frontiers Research Foundation
DOI: 10.3389/fphys.2019.00896

Lamin A/C Mechanotransduction in Laminopathies

Author(s): Francesca Donnaloja, Federica Carnevali, Emanuela Jacchetti, Manuela Teresa Raimondi
Published in: Cells, Issue 9/5, 2020, Page(s) 1306, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells9051306

Advances in Tissue Engineering and Innovative Fabrication Techniques for 3-D-Structures: Translational Applications in Neurodegenerative Diseases

Author(s): Federica Rey, Bianca Barzaghini, Alessandra Nardini, Matteo Bordoni, Gian Vincenzo Zuccotti, Cristina Cereda, Manuela Teresa Raimondi, Stephana Carelli
Published in: Cells, Issue 9/7, 2020, Page(s) 1636, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells9071636

Intellectual Property Rights

Patent (3)

IMPLANTABLE MEDICAL DEVICE

Application/Publication number: 20 18060222
Date: 2018-12-18

SYNTHETIC NICHE MATRICES FOR STEM CELL CULTURE

Application/Publication number: 16 763469
Date: 2016-08-31

A DEVICE FOR CELL CULTURE

Application/Publication number: 17 732566
Date: 2017-05-05