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Sustainable industrial processes based on a C-C bond-forming enzyme platform

Resultado final

Third set of scientific publications

three scientific publications in highquality international refereed journals on CARBAZYME results

Posts, press releases, news items

Coverage of CARBAZYMES activities in social media showcasing IPRcleared information press releases covering CARBAZYMES achievements meetings and workshops and through public presentations during Open Days scientific seminars and lectures to undergraduate or graduate students

First set of scientific publications

two scientific publications in highquality international refereed journals on CARBAZYME results

Fourth set of scientific publications

three scientific publications in high-quality international refereed journals on CARBAZYME results

Project website

Project web site to support project visibility and distribution of information to the public including opportunity for subscription to an enewsletter on specific sections related to every RTD Work Package and report news about events meetings etc

Second set of scientific publications

two scientific publications in highquality international refereed journals on CARBAZYME results

Dissemination and branding standards

Dissemination standards and publicity material that contribute to associate a corporate image to CARBAZYMES and thus contribute to branding which contains the following elements Project logo website layout project flyers layout project slides layout enewsletter layout

Publicity materials

Publicity material to promote visibility of ECsupported research activities of CARBAZYMES at public locations and events

International Outreach Symposium

International Symposium organized (or-co-organized) in the field of biocatalysis for active dissemination of specific CARBAZYME results to stakeholders and technology users in the academic and industrial sectors

Publicaciones

Cover Picture: Nucleophile Promiscuity of Engineered Class II Pyruvate Aldolase YfaU from E. Coli (Angew. Chem. Int. Ed. 14/2018)

Autores: Karel Hernández, Jesús Joglar, Jordi Bujons, Teodor Parella, Pere Clapés
Publicado en: Angewandte Chemie International Edition, Edición 57/14, 2018, Página(s) 3521-3521, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201802275

Mathematical model of the MenD-catalyzed 1,4-addition (Stetter reaction) of α-ketoglutaric acid to acrylonitrile

Autores: Martina Sudar, Đurđa Vasić-Rački, Michael Müller, Alexandra Walter, Zvjezdana Findrik Blažević
Publicado en: Journal of Biotechnology, Edición 268, 2018, Página(s) 71-80, ISSN 0168-1656
Editor: Elsevier BV
DOI: 10.1016/j.jbiotec.2018.01.013

2-Keto-3-Deoxy- l -Rhamnonate Aldolase (YfaU) as Catalyst in Aldol Additions of Pyruvate to Amino Aldehyde Derivatives

Autores: Karel Hernández, Ariadna Gómez, Jesús Joglar, Jordi Bujons, Teodor Parella, Pere Clapés
Publicado en: Advanced Synthesis & Catalysis, Edición 359/12, 2017, Página(s) 2090-2100, ISSN 1615-4150
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/adsc.201700360

Fluorogenic kinetic assay for high-throughput discovery of stereoselective ketoreductases relevant to pharmaceutical synthesis

Autores: Yen-Chi Thai, Anna Szekrenyi, Yuyin Qi, Gary W. Black, Simon J. Charnock, Wolf-Dieter Fessner
Publicado en: Bioorganic & Medicinal Chemistry, Edición 26/7, 2018, Página(s) 1320-1326, ISSN 0968-0896
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.bmc.2017.05.024

Recent advances on halohydrin dehalogenases—from enzyme identification to novel biocatalytic applications

Autores: Anett Schallmey, Marcus Schallmey
Publicado en: Applied Microbiology and Biotechnology, Edición 100/18, 2016, Página(s) 7827-7839, ISSN 0175-7598
Editor: Springer Verlag
DOI: 10.1007/s00253-016-7750-y

The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering

Autores: Jan-Ytzen van der Meer, Lieuwe Biewenga, Gerrit J. Poelarends
Publicado en: ChemBioChem, 2016, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600382

Biocatalytic Aldol Addition of Simple Aliphatic Nucleophiles to Hydroxyaldehydes

Autores: Raquel Roldán, Karel Hernandez, Jesús Joglar, Jordi Bujons, Teodor Parella, Israel Sánchez-Moreno, Virgil Hélaine, Marielle Lemaire, Christine Guérard-Hélaine, Wolf-Dieter Fessner, Pere Clapés
Publicado en: ACS Catalysis, Edición 8/9, 2018, Página(s) 8804-8809, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.8b02486

Exploiting distant homologues for phasing through the generation of compact fragments, local fold refinement and partial solution combination.

Autores: Millán, Claudia; Sammito, Massimo; McCoy, Airlie; Nascimento, Andrey F Ziem; Petrillo, Giovanna; Oeffner, Robert; Domínguez-Gil, Teresa; Hermoso, Juan A; Read, Randy; Usón, Isabel
Publicado en: Acta Crystallographica. Section D, Structural Biology, Edición 6, 2018, Página(s) D74, 290–304, ISSN 2059-7983
Editor: IUCr Journals CRYSTALLOGRAPHY JOURNALS ONLINE
DOI: 10.17863/CAM.25122

Aldolase-Catalyzed Asymmetric Synthesis of N-Heterocycles by Addition of Simple Aliphatic Nucleophiles to Aminoaldehydes

Autores: Raquel Roldán, Karel Hernández, Jesús Joglar, Jordi Bujons, Teodor Parella, Wolf-Dieter Fessner, Pere Clapés
Publicado en: Advanced Synthesis & Catalysis, Edición Adv. Synth. Catal., 2019, Página(s) 361, 1–16, ISSN 1615-4150
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/adsc.201801530

Complete Switch of Reaction Specificity of an Aldolase by Directed Evolution In Vitro: Synthesis of Generic Aliphatic Aldol Products

Autores: Sebastian Junker, Raquel Roldan, Henk-Jan Joosten, Pere Clapés, Wolf-Dieter Fessner
Publicado en: Angewandte Chemie International Edition, Edición 57/32, 2018, Página(s) 10153-10157, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201804831

Nucleophile Promiscuity of Engineered Class II Pyruvate Aldolase YfaU from E. Coli

Autores: Karel Hernández, Jesús Joglar, Jordi Bujons, Teodor Parella, Pere Clapés
Publicado en: Angewandte Chemie, Edición 130/14, 2018, Página(s) 3645-3649, ISSN 0044-8249
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201711289

nanoDSF as screening tool for enzyme libraries and biotechnology development

Autores: Anders O. Magnusson, Anna Szekrenyi, Henk‐Jan Joosten, James Finnigan, Simon Charnock, Wolf‐Dieter Fessner
Publicado en: The FEBS Journal, Edición 286/1, 2018, Página(s) 184-204, ISSN 1742-464X
Editor: Blackwell Publishing Inc.
DOI: 10.1111/febs.14696

Application of Chemical Engineering Methodology in Process Development: A Case Study of MenD-catalyzed Synthesis of 6-Cyano-4-oxohexanoic Acid

Autores: M. Sudar
Publicado en: Chemical and Biochemical Engineering Quarterly, Edición 32/4, 2019, Página(s) 501-510, ISSN 0352-9568
Editor: Hrvatsko Drustvo Kemijskih Inzenjera i Tehnologa/Croatian Society of Chemical Engineers
DOI: 10.15255/cabeq.2018.1393

Structural and Mutagenesis Studies of the Thiamine-Dependent, Ketone-Accepting YerE from Pseudomonas protegens

Autores: Sabrina Hampel, Jan-Patrick Steitz, Anna Baierl, Patrizia Lehwald, Luzia Wiesli, Michael Richter, Alexander Fries, Martina Pohl, Gunter Schneider, Doreen Dobritzsch, Michael Müller
Publicado en: ChemBioChem, Edición 19/21, 2018, Página(s) 2283-2292, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800325

Enantioselective Synthesis of Pharmaceutically Active γ-Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions

Autores: Lieuwe Biewenga, Thangavelu Saravanan, Andreas Kunzendorf, Jan-Ytzen van der Meer, Tjaard Pijning, Pieter G. Tepper, Ronald van Merkerk, Simon J. Charnock, Andy-Mark W. H. Thunnissen, Gerrit J. Poelarends
Publicado en: ACS Catalysis, Edición 9/2, 2019, Página(s) 1503-1513, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.8b04299

2‐Deoxyribose‐5‐phosphate aldolase from Thermotoga maritima in the synthesis of a statin side‐chain precursor: characterization, modeling and optimization

Autores: Anera Švarc, Zvjezdana Findrik Blažević, Đurđa Vasić‐Rački, Anna Szekrenyi, Wolf‐Dieter Fessner, Simon J Charnock, Ana Vrsalović Presečki
Publicado en: Journal of Chemical Technology & Biotechnology, Edición 94/6, 2019, Página(s) 1832-1842, ISSN 0268-2575
Editor: John Wiley & Sons Inc.
DOI: 10.1002/jctb.5956

Selective Ring‐Opening of Di‐Substituted Epoxides Catalysed by Halohydrin Dehalogenases

Autores: Elia Calderini, Julia Wessel, Philipp Süss, Patrick Schrepfer, Rainer Wardenga, Anett Schallmey
Publicado en: ChemCatChem, Edición 11/8, 2019, Página(s) 2099-2106, ISSN 1867-3880
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201900103

Biocatalytic Asymmetric Michael Additions of Nitromethane to α,β-Unsaturated Aldehydes via Enzyme-bound Iminium Ion Intermediates

Autores: Chao Guo, Mohammad Saifuddin, Thangavelu Saravanan, Masih Sharifi, Gerrit J. Poelarends
Publicado en: ACS Catalysis, Edición 9/5, 2019, Página(s) 4369-4373, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.9b00780

Alteration of the Route to Menaquinone towards Isochorismate‐Derived Metabolites

Autores: Alexander Fries, Laura S. Mazzaferro, Björn Grüning, Philippe Bisel, Karin Stibal, Patrick C. F. Buchholz, Jürgen Pleiss, Georg A. Sprenger, Michael Müller
Publicado en: ChemBioChem, 2019, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201900050

Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid

Autores: Karel Hernandez, Jordi Bujons, Jesús Joglar, Simon J. Charnock, Pablo Domínguez de María, Wolf Dieter Fessner, Pere Clapés
Publicado en: ACS Catalysis, Edición 7/3, 2017, Página(s) 1707-1711, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.6b03181

Are in vivo selections on the path to extinction?

Autores: José Berenguer, Mario Mencía, Aurelio Hidalgo
Publicado en: Microbial Biotechnology, Edición 10/1, 2017, Página(s) 46-49, ISSN 1751-7915
Editor: John Wiley & Sons, Ltd
DOI: 10.1111/1751-7915.12490

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