Skip to main content
European Commission logo
français français
CORDIS - Résultats de la recherche de l’UE
CORDIS
CORDIS Web 30th anniversary CORDIS Web 30th anniversary
Contenu archivé le 2024-06-18

Substrate specificity, mechanism and biological roles of rhomboid intramembrane proteases.

Objectif

Rhomboids are widely conserved intramembrane proteases. They are known to control important biological processes in model insect, worm, yeast and protozoan species, but their functions in most organisms including mammals and bacteria are unknown. The key to the understanding of rhomboid functions are their natural substrates, but it has been unclear how these are selected and recognized and methods for substrate identification have been lacking, which is limiting progress in the field.

The core of this proposal aims to discover the biological roles of highly conserved rhomboids by identifying their natural substrates using a combination of advanced substrate specificity analysis, quantitative proteomics and genetics. I will initially focus on representative conserved bacterial rhomboids and identify their substrate repertoire using an in vitro biochemical screen. Subsequent biochemical and genetic analysis in vivo will reveal the biology of the substrates and rhomboids and indicate functions of their orthologues in pathogens. Enzymatic analysis of the identified substrates will elucidate rhomboid specificity and help us solve the three dimensional structure of a rhomboid substrate complex. In a complementary approach, I will use quantitative proteomics to identify substrate repertoire and elucidate substrate specificity of mammalian endoplasmic reticulum (ER) localised rhomboid RHBDL4, which will enable me to understand its biological role in ER-stress-induced cell death, indicated by preliminary experiments.

This project will yield novel biological insights, provide a platform for rhomboid substrate discovery applicable to other biological contexts including pathogens, and provide a deep mechanistic and structural insight into rhomboid protease function, which will help us design new effective rhomboid inhibitors that are needed as experimental tools and have medical potential.

Appel à propositions

FP7-PEOPLE-2011-CIG
Voir d’autres projets de cet appel

Coordinateur

USTAV ORGANICKE CHEMIE A BIOCHEMIE, AV CR, V.V.I.
Contribution de l’UE
€ 100 000,00
Adresse
FLEMINGOVO NAM. 542/2
16610 Praha 6
Tchéquie

Voir sur la carte

Région
Česko Praha Hlavní město Praha
Type d’activité
Research Organisations
Contact administratif
Jitka Silerova (Ms.)
Liens
Coût total
Aucune donnée