Transport derived Ultrafines and the Brain Effects

Deliverables

A folded leaflet containing the main features of the project (presented in a similar way than what is presented on the website) will be produced. This brochure is aimed at presentation of the project e.g. by distribution of the brochure at scientific meetings. An electronic version of the brochure will also be provided on the project website for download. Task 8.2

First online version of network public website

This deliverable describes website and the intranet created for external and internal communication about and on the project. The password protected intranet will serve the purpose of project internal data exchange and information (e.g. on scientific events, reporting deadlines, published papers, repository of previous deliverables and reports). The project’s website will be made publicly available and it will contain information on the project’s partners, current activities and events, recent developments and scientific publications, as well as contact information for stakeholders. Due date: intranet (month 3); public website (month 6) and updates. Task 8.2

Harmonization of the in vitro and in vivo experimentation between partner laboratories

This deliverable is related to earlier D2.2. In addition to in vitro SOP also other key methods will be harmonized. This will enable high comparability between the laboratories for the results. However, in the present project, there are multiple different approaches that are not even possible to be harmonized. This will be covered e.g. by measuring same inflammatory markers from various models and exposure conditions

Report on the investigation of exposure to metal oxides and other biomarkers in 100 cases of AD and 100 controls

Based on the epidemiological studies and comparison to the in vitro and in vivo studies biomarkers in the study subjects are being investigated The success is dependent on the recruitment of study subjects Task 53

Report on acute and long term pulmonary effects of traffic derived UFP in mice

Both acute and longerterm lung effects will be measured and assessed For the shortterm effects no major obstacles are expected however long term effect simulation may be more challenging With all the expertise in the participating organizations it is foreseen that we are able to successfully conduct also this more challenging part Task 25

Report on the comparative toxicities of transportations source pollutants in vitro

At the end of the project we will compile the data and compare the results from different transport modes engines and fuels This is overarching goal of the aerosol toxicology research of the project and we expect to meet the goals in several areas of toxicology research including brain and lung effects as well as genotoxicity in the cells and organs Likelihood for extensive highquality dataset is very high Task 24

Human chamber studies on exposure to traffic related nanoparticles in association with cognitive function and olfactory function

Ume university has long experience on conducting human exposures studies and has the valid license for continuing the work The human studies are greatly adding the significance of the in vitro and in vivo evaluations of the UFP As a result we will have a good overview indicating the effects in olfactory system and cognition after controlled exposures The deliverable is dependent on the recruitment of the study subjects Task 52

Report on the effects of traffic related UFPs on global gene expression and epigenetic profiles of hOMCs

This deliverable refers to task 32 The aim is to identify cellular pathways and DNA methylation patterns altered by UFP exposure in hOMCs Given the experience of partner UEF in culturing hOMCs and partner IEM in NGS and DNA methylation analyses the likelihood of success is high Task 33

Epidemiological studies of traffic related nanoparticles in association with AD and the olfactory system

Results from the epidemiological studies will be based mostly on the reanalyzing the results from the previous studies It will link them to the new findings and the results are potentially giving significant new information of the human health responses to UFP The risk of failure is low Task 51

Advice on mitigation strategies

This task is one of the overarching goals of the project After the extensive emission measurements and the set of respiratory genotoxic and neurotoxic effects of UFP are known These are reported according to the gained results and the content of the advisory report is dependent on the Task 63

Data sets for ambient particle concentrations and size distributions from 1.3 nm to 100 nm.

Due Dates M6, M30 This deliverable is based on the field campaigns in different source environments, traffic, airport, port. TUT has conducted successfully this kind of campaigns previously, thus ensuring high quality of the measured data from different environments. Task 1.2

List of relevant events and stakeholders of the project to be involved in further activities

The relevant events and stakeholders of the project will be identified and listed in the beginning of the project. This deliverable will contain a detailed list of these events and stakeholders. Task 8.1

End-of-project workshop

An endofproject workshop will present the major achievements and conclusions of the project to the stakeholders Feedback will be collected A workshop report will be prepared and published Task 84

Report on acute genotoxicity of very small nanoparticles in vitro and in mice

The report will be prepared from the experiments conducted with the very small nanoparticles in the mice. The results will be compared to the corresponding report from the cell cultures. Both of the exposure conditions being the core of the project, we expect this not to have major hurdles. This can be measured as a ready report. Task 2.3

Report on the neurotoxic, neurodegenerative and AD- promoting effects of UFPs in mice

This report is both an update of the D34 and also an overview of the results on the in vivo experiments of the whole project The report will be compiled between all the partners doing the in vivo experiments The report is based on the available results and the success is mainly based on the collaboration between the partners This report will be done independent on what the project results are thus making the risk of nonexistence negligible This report will provide new potentially groundbreaking results on the UFP effects on the brain Task 36

SOP for ALI-based testing of small nanoparticles

Standard operating procedure for the cell cultures and endpoints in conducting air liquid interface exposes will be prepared. This will increase the comparability between the laboratories. The SOP will include harmonization of cell cultures and measured endpoints. The results will be assessed with sufficient caution, because there are multiple variables affecting the exposure and cell culture conditions. Measure of success is the ready instructions (SOP) for ALI exposures. Task 2.1

Support and collaboration in epidemiology and environmental medicine for investigations of cognitive function in association to air pollution exposure in the study Guangzhou, China

The results from the epidemiological studies in Europe are compared to those in China The extent of Guangzhou study is dependent on the parallel funding in China MOST Both projects will be receiving support from each other and complement the results Task 54

Report on the neurotoxic effects of UFPs in mice following inhalation exposure

This report is focusing on the effects in whole organism with the focus on building link between UFP exposure and adverse effects in central nervous system. It is also acts as the interim update for the project, directing the more mechanistically focused further experiments. Thus, the experiments in the second half of the project are guided by this report, which is making the report an important one. There should not be any obstacles to compile the report. Task 3.4

Initial press release

At the beginning of the project, an initial press release will be prepared describing the overall goals and ambition of the project and announcing the start of the project. The press release will be initially published on the UEF webpage and will be distributed to all project partners for publication on their individual webpages. Task 8.1

Detailed dissemination and communication plan

The Dissemination and Communication Plan outlines the strategy for the dissemination and the communication activities, which will be carried out during the project. This deliverable also details the main dissemination objectives, the target audiences, the communication channels and the dissemination tools. The document include the definitions of the main key performance indicators that will be used to measure the effectiveness and efficiency of the activities carried out. Task 8.1

Report on in depth analysis of the effects of traffic-related UFPs on the NVU

The new cell model D23 is used for the UFP exposures providing new information on the toxicity to the new models The deliverable provides in depth knowledge on the mechanisms related to UFP exposure Task 33

Publications

Lung-depositing surface area (LDSA) of particles in office spaces around Europe: Size distributions, I/O-ratios and infiltration

Author(s): Silvonen V., Salo L., Raunima T., Vojtisek-Lom M., Ondracek J., Topinka J., Schins R.P.F., Lepistö T., Lintusaari H., Saarikoski S., Barreira L.M.F., Hoivala J., Markkula L., Kulmala I., Vinha J., Karjalainen P., Rönkkö T.
Published in: Building and Environment, Issue 246, 2023, Page(s) art. no. 110999, ISSN 0360-1323
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.buildenv.2023.110999

Single-Cell RNA-Seq Analysis of Olfactory Mucosal Cells of Alzheimer’s Disease Patients

Author(s): Riikka Lampinen, Mohammad Feroze Fazaludeen, Simone Avesani, Tiit Örd, Elina Penttilä, Juha-Matti Lehtola,Toni Saari, Sanna Hannonen, Liudmila Saveleva, Emma Kaartinen, Francisco Fernández Acosta, Marcela Cruz-Haces, Heikki Löppönen, Alan Mackay-Sim, Minna U. Kaikkonen, Anne M. Koivisto, Tarja Malm, Anthony R. White, Rosalba Giugno, Sweelin Chew, and Katja M. Kanninen
Published in: Cells, Issue 11, 2022, Page(s) 676, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells11040676

GRAFIMO: variant and haplotype aware motif scanning on pangenome graphs

Author(s): Manuel Tognon; Vincenzo Bonnici; Erik Garrison; Rosalba Giugno; Luca Pinello; Luca Pinello
Published in: Plos Computational Biology, Issue 17(9), 2021, ISSN 1553-734X
Publisher: Public Library of Science
DOI: 10.1101/2021.02.04.429752

In vitro neurotoxicity screening of engine oil- and hydraulic fluid-derived aircraft cabin bleed-air contamination

Author(s): Lora-Sophie Gerber, Regina G.D.M. van Kleef, Paul Fokkens, Flemming R. Cassee, Remco HS Westerink
Published in: NeuroToxicology, Issue Volume 96, 2023, Page(s) 184-196, ISSN 0161-813X
Publisher: Elsevier BV
DOI: 10.1016/j.neuro.2023.04.010

Black carbon toxicity dependence on particle coating: Measurements with a novel cell exposure method

Author(s): Henri Hakkarainen, Laura Salo, Santtu Mikkonen, Sanna Saarikoski, Minna Aurela, Kimmo Teinilä, Mika Ihalainen, Sampsa Martikainen, Petteri Marjanen, Teemu Lepistö, Niina Kuittinen, Karri Saarnio, Päivi Aakko-Saksa, Tobias V. Pfeiffer, Hilkka Timonen, Topi Rönkkö, Pasi I. Jalava
Published in: Science of the Total Environment, Issue 838, 2022, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2022.156543

Toxicity of exhaust emissions from high aromatic and non-aromatic diesel fuels using in vitro ALI exposure system

Author(s): Henri Hakkarainen, Anssi Järvinen, Teemu Lepistö, Laura Salo, Niina Kuittinen, Elmeri Laakkonen, Mo Yang, Maria-Viola Martikainen, Sanna Saarikoski, Minna Aurela, Luis Barreira, Kimmo Teinilä, Mika Ihalainen, Päivi Aakko-Saksa, Hilkka Timonen, Topi Rönkkö, Pasi Jalava
Published in: Science of the total environment, Issue 890, 2023, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2023.164215

Emissions from modern engines induce distinct effects in human olfactory mucosa cells, depending on fuel and aftertreatment

Author(s): Laura Mussalo, Simone Avesani, Muhammad Ali Shahbaz, Ta ́nˇa Za ́vodn ́a, Liudmila Saveleva, Anssi Ja ̈rvinen, Riikka Lampinen, Irina Belaya, Zdenˇek Krejˇcík, Mariia Ivanova, Henri Hakkarainen, Juho Kalapudas, Elina Penttila ̈, Heikki Lo ̈ppo ̈nen, Anne M. Koivisto, Tarja Malm, Jan Topinka, Rosalba Giugnb, Pa ̈ivi Aakko-Saksa, Sweelin Chew, Topi Ro ̈nkko ̈, Pasi Jalava, Katja M. Kan
Published in: Science of the Total Environment, Issue 905, 2023, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2023.167038

Human iPSC-based 3D-Neurosphere Assays for the detection of Developmental Neurotoxicity

Author(s): András Dinnyés, Julianna Kobolák, Mária Bódi-Jakus, Tamás Bellak, Melinda Zana, Anita Fehér, Zofia Janstová
Published in: Toxicology Letters: Official Journal of EUROTOX (Abstracts of the 56th Congress of the European Societies of Toxicology EUROTOX 2021), 2021, ISSN 0378-4274
Publisher: Elsevier BV

Glial smog: Interplay between air pollution and astrocyte-microglia interactions

Author(s): Mireia Gómez-Budia, Henna Konttinen, Liudmila Saveleva, Paula Korhonen, Pasi I. Jalava, Katja M. Kanninen, Tarja Malm
Published in: Neurochemistry International, Issue 136, 2020, Page(s) 104715, ISSN 0197-0186
Publisher: Elsevier BV
DOI: 10.1016/j.neuint.2020.104715

Toxicological evaluation of exhaust emissions from light-duty vehicles using different fuel alternatives in sub-freezing conditions

Author(s): Henri Hakkarainen, Päivi Aakko-Saksa, Maija Sainio, Tuukka Ihantola, Teemu J. Rönkkö, Päivi Koponen, Topi Rönkkö, Pasi I. Jalava
Published in: Particle and Fibre Toxicology, Issue 17/1, 2020, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-020-00348-0

Acute, sub-chronic and chronic exposures to TiO2 and Ag nanoparticles differentially affects neuronal function in vitro

Author(s): Lora-Sophie Gerber, Harm J. Heusinkveld, Celine Langendoen , Burkhard Stahlmecke, Roel PF Schins, Remco HS Westerink
Published in: NeuroToxicology, Issue Volume 93, 2023, Page(s) 311-323, ISSN 0161-813X
Publisher: Elsevier BV
DOI: 10.1016/j.neuro.2022.10.010

The characteristics and size of lung-depositing particles vary significantly between high and low pollution traffic environments

Author(s): Salo L., Hyvärinen A., Jalava P., Teinilä K., Hooda R.K., Datta A., Saarikoski S., Lintusaari H., Lepistö T., Martikainen S., Rostedt A., Sharma V.P., Rahman M.H., Subudhi S., Asmi E., Niemi J.V., Lihavainen H., Lal B., Keskinen J., Kuuluvainen H., Timonen H., Rönkkö T
Published in: Atmospheric Environment, 2021, ISSN 1352-2310
Publisher: Elsevier BV
DOI: 10.1016/j.atmosenv.2021.118421

Culture of Rat Primary Cortical Cells for Microelectrode Array (MEA) Recordings to Screen for Acute and Developmental Neurotoxicity

Author(s): Lora-Sophie Gerber, Lennart V.J. van Melis, Regina G.D.M. van Kleef, Aart de Groot, Remco H.S. Westerink
Published in: Current Protocols, Issue Volume 1, Issue 6, 2021, ISSN 2691-1299
Publisher: Wiley Periodicals LLC
DOI: 10.1002/cpz1.158

Connection between lung deposited surface area (LDSA) and black carbon (BC) concentrations in road traffic and harbour environments

Author(s): Lepistö, T., Kuuluvainen, H., Lintusaari, H., Kuittinen, N., Salo, L., Heli, A., Niemi, J., Manninen, H., Timonen, H., Jalava, P., Saarikoski, S., Rönkkö, T.
Published in: Atmospheric Environment, 2022, ISSN 1352-2310
Publisher: Elsevier BV
DOI: 10.1016/j.atmosenv.2021.118931

Detection and Functional Evaluation of the P2X7 Receptor in hiPSC Derived Neurons and Microglia-Like Cells

Author(s): Linda Francistiová, Kinga Vörös, Zsófia Lovász, András Dinnyés and Julianna Kobolák
Published in: Frontiers in Molecular Neuroscience, Issue 14, 2022, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2021.793769

Cellular and Molecular Effects of SARS-CoV-2 Linking Lung Infection to the Brain.

Author(s): Linda Francistiová; Linda Francistiová; Adrián Klepe; Adrián Klepe; Adrián Klepe; Géza Curley; Géza Curley; Géza Curley; Károly Gulya; András Dinnyés; András Dinnyés; András Dinnyés; Kata Filkor; Kata Filkor; Kata Filkor
Published in: Frontiers in Immunology, Issue 12, 2021, ISSN 1664-3224
Publisher: Frontiers Media SA
DOI: 10.3389/fimmu.2021.730088

PM2.5 exposure and olfactory functions

Author(s): John Andersson,Anna Oudin,Steven Nordin, Bertil Forsberg, Maria Nordin
Published in: INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH, Issue VOL. 32, NO. 11,, 2022, Page(s) 2484-2495, ISSN 0960-3123
Publisher: Chapman & Hall
DOI: 10.1080/09603123.2021.1973969

Particle lung deposited surface area (LDSAal) size distributions in different urban environments and geographical regions: Towards understanding of the PM2.5 dose–response

Author(s): Lepistö T., Lintusaari H., Oudin A., Barreira L.M.F., Niemi J.V., Karjalainen P., Salo L., Silvonen V., Markkula L., Hoivala J., Marjanen P., Martikainen S., Aurela M., Reyes F.R., Oyola P., Kuuluvainen H., Manninen H.E., Schins R.P.F., Vojtisek-Lom M., Ondracek J., Topinka J., Timonen H., Jalava P., Saarikoski S., Rönkkö T.
Published in: Environment International, Issue 180, 2023, Page(s) art. no. 108224, ISSN 1873-6750
Publisher: Elsevier
DOI: 10.1016/j.envint.2023.108224

Snapshots of wintertime urban aerosol characteristics: Local sources emphasized in ultrafine particle number and lung deposited surface area

Author(s): Lepistö T., Barreira L.M.F., Helin A., Niemi J.V., Kuittinen N., Lintusaari H., Silvonen V., Markkula L., Manninen H.E., Timonen H., Jalava P., Saarikoski S., Rönkkö T.
Published in: Environmental Research, Issue 231, 2023, Page(s) art. no. 116068, ISSN 1096-0953
Publisher: Elsevier
DOI: 10.1016/j.envres.2023.116068

Neurotoxicity of Engineered Nanomaterials: Testing Considerations

Author(s): Eleonora Scarcello, Adriana Sofranko, Tina Wahle, Roel P. F. Schins
Published in: Front Public Health, 2022, ISSN 2296-2565
Publisher: Frontiers Media SA
DOI: 10.3389/fpubh.2022.904544

Olfactory cell cultures to investigate health effects of air pollution exposure: Implications for neurodegeneration

Author(s): K.M. Kanninen, R. Lampinen, L.M. Rantanen, L. Odendaal, P. Jalava, S. Chew, A.R. White
Published in: Neurochemistry International, Issue 136, 2020, Page(s) 104729, ISSN 0197-0186
Publisher: Elsevier BV
DOI: 10.1016/j.neuint.2020.104729

Air Pollution and Cognitive Impairment across the Life Course in Humans: A Systematic Review with Specific Focus on Income Level of Study Area

Author(s): Mina Chandra, Chandra Bhushan Rai, Neelam Kumari, Vipindeep Kaur Sandhu, Kalpana Chandra, Murali Krishna, Sri Harsha Kota, Kuljeet Singh Anand and Anna Oudin
Published in: Int. J. Environ. Res. Public Health, Issue 19(3), 2022, ISSN 1660-4601
Publisher: MDPI
DOI: 10.3390/ijerph19031405

Suitability of Different Methods for Measuring Black Carbon Emissions from Marine Engines

Author(s): Päivi Aakko-Saksa; Niina Kuittinen; Timo Murtonen; Päivi Koponen; Minna Aurela; Anssi Järvinen; Kimmo Teinilä; Sanna Saarikoski; Luis M. F. Barreira; Laura Salo; Panu Karjalainen; Ismael K. Ortega; David Delhaye; Kati Lehtoranta; Hannu Vesala; Pasi Jalava; Topi Rönkkö; Hilkka Timonen
Published in: Atmosphere, 2022, ISSN 2073-4433
Publisher: Rotoweb Cantelli
DOI: 10.3390/atmos13010031

Fine and ultrafine airborne PM influence inflammation response of young adults and toxicological responses in vitro

Author(s): Mo Yang, Pasi Jalava, Henri Hakkarainen, Marjut Roponen, Ari Leskinen, Mika Komppula, Guo-Ping Dong, Xiang-Qian Lao, Qi-Zhen Wu, Shu-Li Xu, Li-Zi Lin, Ru-Qing Liu, Li-Wen Hu, Bo-Yi Yang, Xiao-Wen Zeng, Guang-Hui Dong
Published in: Science of the Total Environent, Issue 836, 2022, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2022.155618

PM2.5 and Dementia in a Low Exposure Setting: The Influence of Odor Identification Ability and APOE

Author(s): Andersson John, Sundström Anna, Nordin Maria, Segersson David, Forsberg Bertil, AdolfssonRolf, Oudin Anna.
Published in: Journal of Alzheimer's Disease, Issue Vol. 92, no. 2,, 2023, Page(s) 679-689, ISSN 1387-2877
Publisher: IOS Press
DOI: 10.3233/jad-220469

Characterization of volatile organic compounds and submicron organic aerosol in a traffic environment

Author(s): Saarikoski, S., Hellén, H., Praplan, A. P., Schallhart, S., Clusius, P., Niemi, J. V., Kousa, A., Tykkä, T., Kouznetsov, R., Aurela, M., Salo, L., Rönkkö, T., Barreira, L. M. F., Pirjola, L., Timonen, H.
Published in: Atmospheric Chemistry and Physics, Issue 23, 2023, Page(s) 2963–2982, ISSN 1680-7316
Publisher: European Geophysical Society
DOI: 10.5194/acp-23-2963-2023

Comprehensive characterization of wintertime submicron aerosol in a Nordic town influenced by residential wood combustion, traffic and industrial sources

Author(s): Barreira, L. M. F., Lepistö, T., Salo, L., Helin, A., Aurela, M., Saarikoski, S., Kuittinen, N., Rönkkö, T., Timonen, H.
Published in: Atmospheric Pollution Research, Issue 14, 2023, Page(s) 101835, ISSN 1309-1042
Publisher: Turkish National Committee for Air Pollution Research (TUNCAP)
DOI: 10.1016/j.apr.2023.101835

TUBE project: Transport-derived ultrafines and the brain effects

Author(s): Martikainen, M-V; Aako-Saksa, P; Broek, LD; Cassee, FR; Carare, RO; Chew, S; et al.
Published in: International journal of environmental research and public health, Issue 2, 2022, ISSN 1661-7827
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijerph19010311

TOXICOLOGICAL EFFECTS OF MODERN TRAFFIC EXHAUST: IN VITRO EVALUATION OF EMISSION AFTER-TREATMENT TECHNOLOGIES, ROLE OF FUELS, AND OPERATING TEMPERATURE

Author(s): Hakkarainen Henri
Published in: 2023, ISBN 978-952-61-5050-5
Publisher: University of Eastern Finland